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合成树
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  “合成树”译为未确定词的双语例句
     Each node of the list consists of six fields. Both the generation and drawing of the synthesis tree use the preorder technique.
     合成树采用的是链表存储方式 ,每一个结点由六个域组成 ,且在建树和画树的过程中 ,均用前序遍历 .
短句来源
     Through a actual example, by means of synthesis resin product deelec-trifying and restraint method of free floating pulverized matter cloud, the protective measures are prepared against the electrostatic charge of air conveyed matter.
     通过对空气输送粉体的除电、合成树 脂制品除电和浮游粉尘云的抑制方法,制订了静电带电防范有关措施。
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  相似匹配句对
     Trees
    
短句来源
     Coumarin fluorescent dyes such as C.
     合成了C.
短句来源
     The Trees
    
短句来源
     N,N,N?
     合成了 N,N,N?
短句来源
     K-D Tree-Based Texture Synthesis with Its Applications
     基于K-D的纹理合成及其应用
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查询“合成树”译词为用户自定义的双语例句

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An expert system for chemical synthesis -ESCS was developed, in order to find synthesis paths and steps of one substance. It includes five parts as follows:1. Knowledge notation of chemical reaction and structure of chemical synthesis tree.2. Path segment deduction rules.3. Applications in chemical synthesis.4. Path evaluation method.5. Computation example.

本文为寻求合成某种物质的化学反应途径及步骤,设计出化学合成专家系统ESCS。本文包括如下部分:(1)化学反应式的知识表示与化学合成树的构造;(2)路段演绎规则;(3)在寻找合成途径中的应用;(4)路径优劣评价方法;(5)计算实例。

Objective: To study the gene structures of apolipoproteins of tree shrew (TS) insusceptible to atherosclerosis, the cDNA librariy of tree shrew liver cells was quickly constructed at the first time. Methods: With single -step method, total RNA was isolated from TS liver tissue, following purification of mRNA which passed through oligo-dT cellulose chromatography. Using mRNA as the template- the first and the second strands of the cDNAs were synthesized in a simple modified method. After been ligated with EcoRI/NotI...

Objective: To study the gene structures of apolipoproteins of tree shrew (TS) insusceptible to atherosclerosis, the cDNA librariy of tree shrew liver cells was quickly constructed at the first time. Methods: With single -step method, total RNA was isolated from TS liver tissue, following purification of mRNA which passed through oligo-dT cellulose chromatography. Using mRNA as the template- the first and the second strands of the cDNAs were synthesized in a simple modified method. After been ligated with EcoRI/NotI adapters, the cDNAs were cloned into the arms of λgt10 or λgt11 vectors. Then the recombinants of λDNA were packed by package extract to infect E. coli Y1090 strain. Results: TS liver cDNA librariy with high titer was obtained and rate of positive recombinants was over 98. 5%. Autoradiography showed that sizes of cDNA products were in 400 - 5000 bp. Each white plaque DNA which was identified by PCR contained the cDNA insert. Full length cDNA sequences of TS apoAI and apoCI were cloned and sequenced from the cDNA library constructed. Conclusion: Compared with conventional method of constructing cDNA library the present one is simple, time-saving and easy to be used. Some problems of using the method to construct cDNA library were discussed in detail.

目的:为了研究不易感动脉粥样硬化动物树鼩多种载脂蛋白的基因分子结构,我们在国内外首次快速构建了树鼩肝细胞cDNA文库。方法:采用一步法提取树鼩肝细胞总RNA;以寡核胸苷酸为吸附介质,柱层析法进一步纯化mRNA。以此为模板,利用改良的cDNA快速合成方法,反转录合成树鼩肝细胞cDNA第一、二链。对cDNA双链末端修饰,连接含双酶切位点的连接子后重组于噬菌体载体。结果:包装后获得2.1×10~6高滴度的树鼩肝细胞cDNA文库,放射自显影显示合成的cDNA产物大小在400~5000个核苷酸之间,该文库的克隆重组率为98.5%以上,PCR扩增随机提取的白色噬菌斑DNA,电泳鉴定均有cDNA插入片段。以制备的两种多价抗血清为探针,从构建的cDNA文库中筛选克隆出完整的树鼩apoAI和apoCI的cDNA核苷酸序列。结论:与常规方法比较,本法简便、快速,易于操作和掌握。本研究还对应用此方法构建cDNA文库的某些环节进行了探讨。

Construction of Liver Tissues cDNA Library of Tree Shrew Lu Xinyue; Chen

为了研究动脉粥样硬化不易感动物树多种载脂蛋白的基因分子结构,我们在国内外首次快速构建了树鼯肝组织cDNA文库。采用一步法提取树鼯肝组织总RNA;以寡脱氧胸苷酸纤维素为吸咐介质,柱层析法进一步纯化mRNA。以此为模板。利用改良的cDNA快速合成方法,反转录合成树鼯肝组织cDNA第一、二链。对cDNA双裢末端修饰,连接含双酶切位点的连接子后重组于噬菌体载体,包装后获得2.1x106高滴度的树肝组织cDNA文库,放射自显影显示合成的cDNA产物大小在400-5000个核苷酸之间,该文库的克隆重组率为98.5%以上,扩增随机提取的白色噬菌斑DNA,电泳鉴定均有cDNA插入片段。以制备的两种多价抗血清为探针,从构建的cDNA文库中筛选克隆出完整的树apoAI和apoCI的cDNA核苷酸序列。与常规方法比较,本法简便、快速、重复性好,易于操作和掌握,值得推广使用。

 
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