The pre-embedding immunoelectron microscopic method was used to study the development of antibody-producing cells in the guinea pig popliteal lymph nodes of 2,3,5,8 and 10 days after a second challenge with horseradish peroxidase.
there were 2.54±? 0.79 in blood, 77.48±3.14 in politeal lymphnodes, 6.12±0.73 in kideys, below 1 in other organs about distribution in vivo. The development of inguninal lymph nodes and popliteal lymph nodes was clear.
Methods We injected complete or incomplete freurd adjuvant into the rear footpads of the mice and the popliteal lymph nodes were took out a few days later, and processed with PNA-B and immunohistochemistry(PAP). We used reconstitution of three dimensions to count subpopulation of FDCs.
Another 7 days later, both popliteal lymph nodes were prepared, weighed, the cell number was counted and the quotients ("index") of these variables from the treated and the untreated side were determined.
The draining popliteal lymph nodes (PLN) were harvested 1-4 days posttreatment, labeled with CFSE and cultured for 1-4 days without further stimulation or in the presence of the recall antigen.
Successfully immunized recipients were identified by the enlargement of their popliteal lymph nodes following footpad challenge with spleen cells of the donor type.
At the end of the study the rabbits were killed and the popliteal lymph nodes and the organs were removed to be weighed, and counted.
The time course of signal loss in popliteal lymph nodes of 21 rats was studied before and up to 72 h after IV injection of SPIO.