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氯仿熏蒸法
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  “氯仿熏蒸法”译为未确定词的双语例句
     In this paper,soil microbial community properties of five vegetation patterns were measured by bacterial,microbial biomass carbon and metabolic diversity (BIOLOG) assays to evaluate the effects of different forest restoration patterns on soil microbial community.
     为了评价不同森林恢复类型与方式对南方红壤丘陵区退化生态系统土壤微生物群落的影响 ,借助氯仿熏蒸法、平板涂抹法和BIOLOG检测法 ,比较研究了 4种森林恢复类型土壤微生物的群落特征 .
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     It was sensitive to chloroform.
     对氯仿敏感;
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     It is sensitive to chloroform.
     对氯仿敏感;
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     STUDY ON ERADICATING PEST IN FIVE-DAMAGED LOGS WITH FUMIGATING METHOD AND SPRAYING METHOD
     熏蒸法治理火烧原木害虫研究
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     On the Amount of Formaldehyde Used to Air Oxidizing Fumigation
     甲醛氧化熏蒸法空气消毒用量探讨
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  chloroform fumigation
Microbial-feeders were in general associated with both bacterial and fungal PLFA, microbial biomass C (MBC) by chloroform fumigation-extraction, total C and N, NH4+ and NO3-, and were most abundant in the surface soil of the NTCC treatment.
      
The chloroform fumigation-incubation method (CFIM) was used to measure the microbial biomass of 17 agricultural soils from Punjab Pakistan which represented different agricultural soil series.
      
In experiments with saline soil which was initially sterilized with chloroform fumigation, a mixed culture of N2-fixing rhizospheric isolates from kallar grass roots was inoculated and planted to kallar grass.
      
This discrepancy was attributed to errors in the chloroform fumigation technique.
      
In acid soil, the chloroform fumigation-incubation method failed to estimate the microbial biomass, not because bacterial growth was inhibited after fumigation but because a labile C source was taken up which differed from the killed biomass C.
      
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In this paper,soil microbial community properties of five vegetation patterns were measured by bacterial,microbial biomass carbon and metabolic diversity (BIOLOG) assays to evaluate the effects of different forest restoration patterns on soil microbial community.The results showed that among five sampling sites,soil microbial biomass carbon and bacterial amount were the highest under natural secondary forest,while those in CK were the least.In BIOLOG assays,the average well colour development (AWCD) and the...

In this paper,soil microbial community properties of five vegetation patterns were measured by bacterial,microbial biomass carbon and metabolic diversity (BIOLOG) assays to evaluate the effects of different forest restoration patterns on soil microbial community.The results showed that among five sampling sites,soil microbial biomass carbon and bacterial amount were the highest under natural secondary forest,while those in CK were the least.In BIOLOG assays,the average well colour development (AWCD) and the richness and diversity indices indicated the differences of microbial metabolic diversity.The variation tendency of the indices was the same as that of microbial biomass and bacterial amount.It is suggested that under natural secondary forest,soil microbial community had a stronger ability of utilizing carbon source and a greater functional diversity than that in plantations and CK.The metabolic diversity of microbial communities in 0~20 and 20~40 cm soil layers showed a fairly good correlation with root biomass (r=0.933,P<0.05;r=0.925,P<0.05).In a word,soil microbial biomass,bacterial amount and metabolic diversity patterns were the highest under natural secondary forest,and natural restoration should be more propitious to improve the structure and function of soil microbial community.

为了评价不同森林恢复类型与方式对南方红壤丘陵区退化生态系统土壤微生物群落的影响 ,借助氯仿熏蒸法、平板涂抹法和BIOLOG检测法 ,比较研究了 4种森林恢复类型土壤微生物的群落特征 .结果表明 ,4种森林恢复类型土壤微生物生物量碳、细菌数量差异显著 ,2项指标均以天然次生林土壤最高 ,人工林次之 ,荒地最差 ;碳源平均颜色变化率 (AWCD法 )和微生物代谢多样性指数 (丰富度和多样性 )在 5种植被类型的土壤中也有明显差异 ,其趋势与微生物量碳、细菌数量基本相同 ;天然次生林土壤微生物群落利用碳源的整体能力和功能多样性比人工林和荒地强 .相关分析表明 ,0~ 2 0和 2 0~ 4 0cm土壤微生物的代谢多样性与根系生物量紧密相关 (r =0 933,P <0 0 5 ;r =0 92 5 ,P <0 0 5 ) .自然恢复更有利于改善土壤微生物的结构和功能 .

A chloroform-fumigation extraction—water bath method(CFEB) was developed for measuring microbial biomass C in waterlogged soils.Liquid chloroform was directly added to the waterlogged soil to be tested,which was fumigated for 24 h at 25 ℃ under normal atmospheric pressure and in darkness,and then were extracted with 0.5mol L~(-1) K_(2)SO_(4) solution.The extractions were carried out in 100℃ water bath for 45~60 min to remove chloroform residue before measuring TOC.Non-fumigation controls were extracted directly...

A chloroform-fumigation extraction—water bath method(CFEB) was developed for measuring microbial biomass C in waterlogged soils.Liquid chloroform was directly added to the waterlogged soil to be tested,which was fumigated for 24 h at 25 ℃ under normal atmospheric pressure and in darkness,and then were extracted with 0.5mol L~(-1) K_(2)SO_(4) solution.The extractions were carried out in 100℃ water bath for 45~60 min to remove chloroform residue before measuring TOC.Non-fumigation controls were extracted directly with 0.5 mol L~(-1) K_(2)SO_(4) solution.The method was tested on seven paddy soils which were incubated aerobically or anaerobically and compared with the standard chloroform fumigation extraction(CFE).The results of the tests were reliable and reproducible,suggesting that the CFEB is a rapid and effective method for measuring microbial biomass C in waterlogged soils.

针对氯仿熏蒸浸提法测定淹水土壤微生物生物量碳时存在的问题,进行了测定方法的研究,建立了适合淹水土壤微生物生物量碳测定的新方法:液氯熏蒸浸提—水浴法,即在淹水土壤加入一定量的液态氯仿后,直接置于常压下熏蒸,然后用0.5mol L-1K2SO4溶液浸提,随后将浸提液放置于100℃水浴中以排除其中的残余氯仿,最后采用TOC分析仪测定浸提液中的有机碳含量。本方法既符合氯仿熏蒸法的原理,在操作上又简便可行,是一种测定淹水土壤微生物生物量碳的理想方法。

castnea mollissima forest is an important economic forest that has been intensively managed in southern China recently. Consequently, soil quality was getting worse and worse with the intensive management. The effects of C. mollissima intensive management on soil biological properties was studied by sampling soil from C. mollissima stands with different history of intensive management in Anji City, Zhejiang Province. Three soil samples from natural shrubbery were collected. The soil microbial biomass carbon...

castnea mollissima forest is an important economic forest that has been intensively managed in southern China recently. Consequently, soil quality was getting worse and worse with the intensive management. The effects of C. mollissima intensive management on soil biological properties was studied by sampling soil from C. mollissima stands with different history of intensive management in Anji City, Zhejiang Province. Three soil samples from natural shrubbery were collected. The soil microbial biomass carbon under C. mollissima stand with 5,10 and 20 year's intensive management were decreased by 15.89%,49.16% and 55.13% respectively, and the differents were significant (P<0.05). There is no significant difference between the proportions of soil microbial biomass carbon to soil total organic carbon under the C. mollissima stand during 5 years intensive management and the natural shrubbery, however, it decreased apparently until 10-year's intensive management and then kept constant afterward. Compared with shrubbery, The soil AWCD (average well color density)value under C. mollissima stand decreased by 79.26%, 63.20% and 68.50% respectively after 5, 10 and 20-year's intensive management, with significant difference(P<0.05). Indexes of Shannon and McIntosh reflecting soil microorganism diversity were lower in intensive management C. mollissima stands than that in natural shrubbery. Combining AWCD value with diversity indexes, the soil microbial functional diversity of intensive management C. mollissima stand was decreased noticeably during the first-10 year's intensive management, but no change observed afterward.

在研究区采集不同栽培历史的板栗林土壤样品,用氯仿熏蒸法和Biolog法分析土壤的微生物量碳和微生物多样性,同时与天然灌木林进行比较。结果表明板栗林集约化栽培5、10、20年后,土壤微生物量碳含量比天然灌木林下降了15.89%、49.16%和55.13%,差异显著。板栗林集约栽培初期,土壤微生物量碳占总有机碳比率与天然灌木林无显著差异;到集约经营10年时,比率明显下降;但集约经营10年后,比率又趋于稳定。灌木林改为集约栽培板栗林后,土壤微生物群落功能多样性呈下降趋势,板栗集约经营5、10和20年后,反映土壤微生物碳源利用能力的AWCD值分别只是灌木林的79.26%、63.20%和68.50%,差异水平显著(P<0.05)。集约栽培板栗林后,反映土壤微生物多样性的Shannon指数和McIntosh指数也显著低于天然灌木林。综合AWCD值和多样性指数可以看出,板栗林集约栽培的头10年里,土壤微生物群落功能多样性下降趋势明显,10年后则变化不大。

 
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