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|  | | 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。 | |
The mode of action of IVIG may range from direct anti-idiotype effect to indirect effects on the cellular part of the pathogenesis of paraneoplastic syndromes.
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A monoclonal IgG1 anti-idiotype, specific for the K13 polysaccharide combining site of a protective IgM idiotype, primed for protection against intraperitoneal infection with liveE.
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The monoclonal anti-K13 idiotype only primed for protection at four weeks of age.
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These findings suggest a strong effect of a single idiotype on the outcome of a bacterial infection.
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A monoclonal IgG1 anti-idiotype, specific for the K13 polysaccharide combining site of a protective IgM idiotype, primed for protection against intraperitoneal infection with liveE.
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| | The affinity column purified anti-DNP antibodies from secondary response of a single Wistar rat were used to immunize a rabbit. The serum of which was first absorbed with immunoglobulins of normal Wistar rats and then precipitate with ammonium sulphate. The anti-idiotypic specificities of the antibodies thus obtained were identified with ELISA, in which the walls of plate were coated with affinity column purified anti-DNP antibodies. With self Ig separated from preimmunized serum as control, the results sho... | | 本文以“逆向”ELISA 测定 Wistar 大鼠抗 DNP抗血清中抗体的独特型,即以抗独特型抗体为涂布物来测定抗血清的效价。实验结果表明“逆向”ELISA可以专一地半定量测定大鼠抗 DNP抗血清的独特型,为进一步研究抗独特型抗体对抗体独特型表达的调节作用打下基础。同时,DNP-BSA封闭大鼠抗 DNP抗体的实验初步表明,这抗体的独特型决定簇位于抗体分子的抗原结合部位,至少十分接近抗原结合部位。 | | 文摘来源 | | A series of hybridoma cell lines producing monoclonal antibodies to DNA had been obtained by hybridization of spleen cells from MRL-1Pr/1pr mice, which had developed a severe form of SLE and spontaneously produced high levels of antibodies to DNA without exogenus immunization, with SP_2/0-Ag-14 plasmacytomas. The McAb H241, one of those monoclonal antibodies, was used to immunize NZW rabbit and an anti-H241 idiotypic antibody was harvested from immune serum of the rabbit successfully. The sensitivity of H24... | | 由严重存在SLE,并无需外源免疫能自发产生抗DNA自身抗体的小鼠MRL-Lpr/Lpr脾细胞与SP2/0-Ag-14骨髓瘤细胞杂交,获得一系列杂交瘤细胞株,它们分别分泌单克隆抗DNA自身抗体。我们用其中的细胞株H241的单抗免疫家兔,从免疫血清中收获到抗H241独特型扰体。通过放射免疫分析法(RIA),对H241进行了敏感度分析,并研究了H241与抗H211血清反应中,H241与另24个抗DNA单抗独特型交叉反应。结果表明,H241是高敏感度的单抗,6ng非标记H241能使~(125)I-H241与抗H241独特型抗体的结合达50%抑制。实验还证明,24个抗DNA单抗中有15个与H241不同程度地存在独特型交叉反应。实验提示,虽然抗DNA自身抗体独特型往往结构分离,从而表现抗体多样性,但有些抗体由于独特型结构类似,多样性受到限制;全身性红斑狠疮小鼠控制免疫球蛋白合成的胚系基因含有某些抗DNA抗体的相关位点。 | | 文摘来源 | | Objective To probe into immune pathogeny of recurrent spontaneous abortion (RSA). Methods Immunological analysis was made in ABO group antibodies by indirect hemagglutination, anti zona pellucida antibodies by BA ELISA, anti phospholipid antibodies by ELISA, blocking effect (MLR BE) by blocking test one way mixed lymphocyte reaction, cytotoxic index (CI) by complement dependent cytotoxic test, anti idiotypic antibodies to blocking antibodies by MLR BE inhibiting test, and effects of blocking antib... | | 目的分析反复自然流产(RSA)的免疫病因组成。方法用间接血凝法检测ABO血型抗体,用BA-ELISA法检测透明带抗体,用ELISA法检测磷脂抗体,以单向混合淋巴细胞反应封闭试验检测封闭效率,通过补体依赖性细胞毒试验测定细胞毒指数,通过单向混合淋巴细胞反应封闭抑制试验分析封闭抗体的抗独特型抗体,利用流式细胞仪分析封闭抗体对CD抗原的作用。以正常生育组为对照,回顾性分析了129例原发性RSA及16例继发性RSA的免疫病因构成。结果在RSA病因组成中,封闭抗体缺乏占31.7%,透明带自身抗体占20.4%,磷脂抗体占12.5%,ABO血型抗体占11.5%;在原发性流产病因组成中,封闭抗体缺乏占31.4%,透明带自身抗体占20.4%,磷脂抗体占8.5%,ABO血型抗体占8.4%;在继发性流产中,封闭抗体独特型-抗独特型网络失调占39.4%,磷脂抗体占31.4%,ABO血型抗体占22.4%。结论根... | | 文摘来源 | |   | | << 更多相关文摘 |
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