sp. trittici into field wheat at elongating,booting and flowering stages,the result showed that infection stages did not affect the expression of latent period,infection efficiency,sprorulation capacity,uredial size and yield components of wheat. The slow-leaf rusting of wheat cultivars expressed at all development stages of the hosts. Early or late occurence of wheat leaf rust had no effect on the expression of low-leaf rusting.
These results suggest that βC1 gene is a pathogenicity determinant and contributes to the infectivity of the disease complex of TYLCCNV and the satellite DNAβ.
The highest transformation efficiency was obtained when the bacterial cell density was OD600 0.3-0.5 with 5-10 minutes of infection. It was also higher to transformation efficiency under no pre-culture and co-culture for 3-4 days and AS concentration with10mg/L.
tabacum Samsun, N. tabacum and Lycopersicon esculentum plants. The wild type Y10 DNAp co-agroinoculated with TYLCCNV-Y10 caused systemic infection in all the above hosts and induced severe disease symptoms.
glutinosa plants and could replicate when co-agroinoculation with other geminiviruses. The function of βC1 gene of DNAβ associated with Tobacco curly shoot virus isolate Y35 (TbCSV-Y35) was studied. A βCl truncated Y35 DNAβ was constructed.
The results showed that for vHa+egfp, one TCID50 unit tested by end point dilution assay was equivalent to 103 copies of viral genome DNA. For vHa+gp64+egfp, however, one TCID50 unit was equivalent to 102 copies of viral genome DNA.
The inheritance of host plant resistance and its effect on the relative infection efficiency for leaf blast was studied in the crosses 'IR36'/'CO39' (partially resistant × highly susceptible) and 'IR36'/'IR64' (both partially resistant).