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印度蜜蜂
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  indian bee
     A biological study on diploid drones produced by \%Apis cerana cerana\% shows,under the condition of high inbreeding,diploid drone’s imago ratio is higher than that of Western bee and Indian bee, its figureand biological characters have apparent differences with haploid drones.
     对中华蜜蜂产生二倍体雄蜂的生物学研究表明,在高度近亲繁殖(兄妹交配的蜂王)条件下,中华蜜蜂产生二倍体雄蜂,二倍体雄蜂的成虫率高于西方蜜蜂和印度蜜蜂,形态特征和生物学特性与单倍体雄蜂存在明显差异。
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  相似匹配句对
     The Developing India
     印度笔记
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     SECURITIES MARKET OF INDIA
     印度证券市场
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     The Eagle and the Bee
     老鹰和蜜蜂
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     Bee
     小蜜蜂
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     AN AMEROSEIID MITE——NEOCYPHOLAELAPS INDICA EVANS FROM APIS MELLIFERA
     蜜蜂体上的印度新曲厉螨(Neocypholaelaps indica Evans,1963)(Acarina:Ameroseiidae)
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  indian bee
The autochtonous bee in the taiga of these regions was the Indian bee, Apis cerana, here called Ussuriyan bee.
      


Two cDNA fragments encoding prepromelittin were amplified by RT-PCR fr om the total RNA from the venom glands of the worker honeybees, Apis cerana cerana and A. mellifera. The PCR products were ligated into pGEM-T easy vector. The results of sequencing showed that the ORFs coding for prepromel ittin were all 213 bp in length. The amino acid sequence of A. cerana cerana prepromelitti n shared 97% homologies with that of A. mellifera prepromelittin and A. ce rana indica prepromelittin. The nucleotide sequence...

Two cDNA fragments encoding prepromelittin were amplified by RT-PCR fr om the total RNA from the venom glands of the worker honeybees, Apis cerana cerana and A. mellifera. The PCR products were ligated into pGEM-T easy vector. The results of sequencing showed that the ORFs coding for prepromel ittin were all 213 bp in length. The amino acid sequence of A. cerana cerana prepromelitti n shared 97% homologies with that of A. mellifera prepromelittin and A. ce rana indica prepromelittin. The nucleotide sequence reported here was submitte d to GenBank with accession no. AF487907.

分别从中华蜜蜂Apisceranacerana和意大利蜜蜂Apismellifera工蜂毒腺中抽提总RNA ,通过RT PCR方法扩增 ,各得到了蜂毒前溶血肽原蛋白的cDNA ,再将扩增产物克隆到pGEM Teasy载体上 ,进行测序和序列分析。结果表明 ,所扩增到的这两个片段长度均为 2 13bp ,均为编码蜂毒前溶血肽原的cDNA ,并分别推导出两者所编码的氨基酸序列。经序列比较 ,中华蜜蜂前溶血肽原与意大利蜜蜂、印度蜜蜂Apisceranaindica前溶血肽原的同源性都为 97%。所报道的中华蜜蜂蜂毒前溶血肽原的核苷酸序列的GenBank登录号为AF48790 7。

A biological study on diploid drones produced by \%Apis cerana cerana\% shows,under the condition of high inbreeding,diploid drone’s imago ratio is higher than that of Western bee and Indian bee, its figureand biological characters have apparent differences with haploid drones. Sperm of diploid drones produced by \%Apis cerana cerana\% is small in quantity, low in density, poor in activity and high in death rate.

对中华蜜蜂产生二倍体雄蜂的生物学研究表明,在高度近亲繁殖(兄妹交配的蜂王)条件下,中华蜜蜂产生二倍体雄蜂,二倍体雄蜂的成虫率高于西方蜜蜂和印度蜜蜂,形态特征和生物学特性与单倍体雄蜂存在明显差异。中蜂二倍体雄蜂排精量少,精子密度低,精子总数少,精子活力较差,死精率高。

A cDNA library from worker heads of Apis cerana cerana was constructed and a total number of 8568 expression sequence tags(ESTs) were definite.Ninety-six clones containing Apisimin gene were identified from the total ESTs,indicating that Apisimin gene is a highly transcriptional gene.The total length of the cDNA was 379 nt,containing an open-reading frame(ORF) of 237 nt.The putative ORF might encode a 78 amino acid residue precursor,which is consisted of a 24 amino acid signal peptide in the N-terminal and a...

A cDNA library from worker heads of Apis cerana cerana was constructed and a total number of 8568 expression sequence tags(ESTs) were definite.Ninety-six clones containing Apisimin gene were identified from the total ESTs,indicating that Apisimin gene is a highly transcriptional gene.The total length of the cDNA was 379 nt,containing an open-reading frame(ORF) of 237 nt.The putative ORF might encode a 78 amino acid residue precursor,which is consisted of a 24 amino acid signal peptide in the N-terminal and a 54 residue mature peptide with a predicted molecular weight of 5.9 kDa in the C-terminal.The Apisimin gene shared 100 % and 95% of homologies with A.mellifera and A.cerana indica at nucleotide level,respectively.The coding region of the matured peptide was sub-cloned into the prokaryotic expression vector pGEX-4T-2 to fuse with GST to form a recombinant vector pGEX/apisimin.The recombinant was then transformed into E.coli BL21(DE3) for fusion expression.The SDS-PAGE and thin-layer scanning showed that the expressed GST-apasimin fusion protein was about 31 kDa in size and about 22.1% in proportion to the total bacterial proteins as well as about 50% soluble.The fusion protein was further purified through affinity chromatography.

构建中华蜜蜂(Apis cerana cerana)工蜂头部cDNA文库并进行了测序,从8568个表达序列标签(expression sequencetag,EST)中获得96个Apisimin基因克隆,结果表明Apisimin基因是一个高丰度转录的基因。进一步对克隆分析表明该基因cDNA全长为379 nt,包含一个237 nt、可以编码78个氨基酸的ORF。推导的Apisimin蛋白N端含有一个由24个氨基酸残基组成的信号肽,后端为54残基、预计分子量为5.9kDa的成熟肽。该ORF与意大利蜜蜂和印度蜜蜂的Apisimin基因核苷酸同源性分别为100%和95%。将成熟肽编码区进行亚克隆,构建了Apisimin与谷胱甘肽转移酶融合表达的载体pGEX/apisimin,并将重组载体转化入大肠杆菌BL21(DE3)进行融合表达。SDS-PAGE和薄层扫描表明,表达的融合蛋白分子量为31 kDa,表达量占细菌总蛋白的22.1%,约50%是可溶性的。通过亲和柱进一步纯化了表达的融合蛋白。

 
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