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| This paper reviewed the major research achievements on the germp-lasm resources, new variety development , plant introduction , cultural techniques, tissue culture, storage, and plant protection of vegetable crops obtained during the last 35 Years after the founding of the People's Re-public of China.The progress in some aspects was also presented, includ-ing the genetic behavior of the major economical charactristics, self-in -compatibility,male sterility, and the gynoecium of major vegetable crops, the isoenzymogram... This paper reviewed the major research achievements on the germp-lasm resources, new variety development , plant introduction , cultural techniques, tissue culture, storage, and plant protection of vegetable crops obtained during the last 35 Years after the founding of the People's Re-public of China.The progress in some aspects was also presented, includ-ing the genetic behavior of the major economical charactristics, self-in -compatibility,male sterility, and the gynoecium of major vegetable crops, the isoenzymogram of the peroxidase in F1 hybrids of tomato and Chine-se cabbage, and the cytology of the common yellow daylily and Chinese chive, etc. | 本文概述建国三十五年来我国在蔬菜种质资源、新品种选育、蔬菜引种、栽培技术、组织培养、贮存保鲜、植物保护等研究工作的主要成就。几种主要蔬菜重要经济性状及自交不亲和性、雄性不育性、全雌性等遗传表现;番茄、大白菜一代杂种过氧化物酶同工酶酶谱分析;黄花菜、韭菜细胞学研究等方面取得的成绩。 | Light quality affected not only the callus growth and organogenesis but also the contents of carbohydrate,protein,DNA and chlorophyll.The highest protein and chlorophyll contents in white light were observed while blue light and dark inhibited protein synthesis.Tissue in green light was of a higher growth value but less carbohydrate content and it was just the opposite in dark.It is reasonable that the effects of light quality on plant tissue culture had no direct relationship with photosynthesis.The photomorphogenesis... Light quality affected not only the callus growth and organogenesis but also the contents of carbohydrate,protein,DNA and chlorophyll.The highest protein and chlorophyll contents in white light were observed while blue light and dark inhibited protein synthesis.Tissue in green light was of a higher growth value but less carbohydrate content and it was just the opposite in dark.It is reasonable that the effects of light quality on plant tissue culture had no direct relationship with photosynthesis.The photomorphogenesis may be influenced by light quality through carbohydrate transformation.DNA contents also varied dramatically with light quality and showed a significant negative relationship with growth value. The specific activity of paroxidase was somehow related with the differentia- tion stage.Tissues of higher organogenesis activies in white,red light and dak were also of higher specific activities of the enzyme while the tissues in yellow and green light in which shoots had differentiated were of lower activities. The isozyme pattern of dank treatment contained an extra P_x4 band in comparing with light treatments.Light quality affected the strength of bands rather than the presence of bands P_x1 to3.This indicated that the effect of light quality on tissue culture occured not only in genes sequentis expression but also in pas- ttranscriptional process. | 光质不仅对毛地黄(Digitalis purpurea)叶愈伤组织的生长及器官发生有影响,同时也显著地影响了组织培养物中多糖类、蛋白质、DNA、叶绿素等生物大分子物质的含量。白光中蛋白质和叶绿素含量均较高,而蓝光、黑暗却抑制蛋白质的合成。生长值较高的绿光中含糖量较少,而生长值较低的黑暗中却有较多的糖。由此设想,光质对组织培养的影响与光合作用并无直接关系,光质可影响糖的转化过程,从而影响光形态建成。DNA 含量随光质的不同也有很大差异,并与生长值之间存在着显著的负相关。过氧化物酶活力测定表明,其比活力与形态发生有关,正处于器官发生高峰期的白光、黑暗、红光处理中,酶的比活力较高,而已有大量芽产生的黄、绿光处理组,其酶比活力较低。同工酶酶谱分析结果表明,黑暗中比有光条件下多一条 Px4谱带,各种光质既可影响到 Px1—3酶谱带的出现,也可影响各条带的宽窄和颜色深浅。这说明,光质对组织培养的影响,不但反映在基因的顺序表达水平上,而且也可作用在转录后的过程中。 | Peroxidase isozyme patterns in 23 materials of Ipomoea Genus (inch ding 10 diploid wild species,2 tetraploid wild species,7 hexaploid wild strains and 4 hexaploid cultivars) were investigated via slab polyacrylamide gel electrophoresis technique.The primary results showed that zymogram variation could be found obviously among different species especially in diploid and tetraploid wild species rather than in different hexaploid wild strains or in different hexaploid cultivars.However,4 dominant bands,Pb2,Pb3,Pcl... Peroxidase isozyme patterns in 23 materials of Ipomoea Genus (inch ding 10 diploid wild species,2 tetraploid wild species,7 hexaploid wild strains and 4 hexaploid cultivars) were investigated via slab polyacrylamide gel electrophoresis technique.The primary results showed that zymogram variation could be found obviously among different species especially in diploid and tetraploid wild species rather than in different hexaploid wild strains or in different hexaploid cultivars.However,4 dominant bands,Pb2,Pb3,Pcl and Pc2,were presented in trifida (6x),l.trifida (2x),I.littoralis (4x) and l.batatas L.(sweet potato) simultaneously which could be considered as the marked bands of B group species in Ipomoea batatas section. | 采用垂直平板聚丙烯酰胺凝胶电泳技术,对23份甘薯属不同倍性材料进行过氧化物酶同工酶酶谱分析。初步结果表明,过氧化物酶同工酶酶带数目与材料倍性无明显相关性;二倍体或四倍体野生种的种间酶谱差异显著;六倍体野生种不同株系间以及六倍体栽培种甘薯的不同品种间酶谱差异较小;但栽培种甘薯与六倍体野生种I.trifida(6x)、四倍体野生种I.littoralis(4x)以及二倍体野生种I.trifida(2x)的酶谱有4条明显共同标记带,表明其间有一定亲缘关系。 | | | | << 更多相关文摘 |
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同工酶酶谱分析
analysis of isoenzyme patterns on progenies
查询“同工酶酶谱分析”译词为用户自定义的双语例句
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