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晶体     
相关语句
  crystal
    Protein Crystal Growth and Nucleation Observed by Atomic Force Microscopy (AFM)
    用原子力显微镜(AFM)进行蛋白质晶体生长及成核研究
短句来源
    Purification, Characterization, Crystal Growth and Identification of Nitrogenase MoFe Protein from Mutant DJ35 and Bacterioferritin from UW3 of Azotobacter Vinelandii
    固氮菌突变种DJ35钼铁蛋白和UW3细菌铁蛋白的纯化、特性、晶体生长及鉴定
短句来源
    Purification, Characterization and Crystal Growth of MoFe Protein and HBP59 from a NifE-deletion Strain DJ35 of Azotobacter Vinelandii
    棕色固氮菌突变株DJ35中钼铁蛋白和HBP59蛋白的研究—纯化、特性及晶体生长
短句来源
    STRUCTURAL STUDIES ON(Trp)~(B_1) INSULIN Ⅰ. CRYSTAL GROWTH AND PRELIMINARY CRYSTALLOGRAPHIC OBSERVATION
    [Trp]~(B_1)胰岛素结构研究——Ⅰ.晶体生长与初步晶体学观察
短句来源
    STRUCTURAL STUDIES ON (Trp)~(B_1) INSULIN——Ⅰ.CRYSTAL GROWTH AND PRELIMINARY CRYSTALLOGRAPHIC OBSERVATION
    [Trp]~(B_1)胰岛素结构研究——Ⅰ.晶体生长与初步晶体学观察
短句来源
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  crystals
    STRUCTURAL STUDIES ON DES-(B-CHAIN C-TERMINAL PENTAPEPTIDE)INSULIN——Ⅱ. GROWTH OF CRYSTALS AND PRELIMINARY CRYSTALLOGRAPHIC OBSERVATIONS
    去B链羧端五肽胰岛素的结构研究——Ⅱ.晶体生长及其初步结晶学观察
短句来源
    ELECTROPHORETIC ANALYSIS ON THE ESTERASE ISOENZYME FROM THE CRYSTALS OF THE FISH EYES
    鱼眼晶体酯酶同工酶电泳分析
短句来源
    A Study on the Quality of LPE In_(1-x)Gal_xA syP_(1-y)Crystals by IR Photoluminscence Method
    红外光谱法检测LPE In_(1-x)Gal_xA syP_(1-y)晶体质量优劣的研究
短句来源
    PROTEIN COMPONENTS AND TOXICITY OF DELTAENDOTOXIN CRYSTALS FROM BACILLUS THURINGIENSIS Tm13-14
    苏芸金杆菌广谱杀虫Tm13-14菌株晶体毒素及毒力特性
短句来源
    The crystals of PC were obtained by hanging drop vapor diffusion method and the size of some crystals was up to 0.1x0.5x0.6 mm.
    通过悬滴气相扩散法对藻蓝蛋白进行晶体培养,获得了大小为0.1×0.5×0.6 mm的晶体.
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  lens
    SEM Observation on Lens Fibers of Congenital Cataract of BALB/c Mice
    先天性白内障BALB/c小鼠的晶体纤维扫描电镜观察
短句来源
    Effect of Selenite on αA-Crystallin Gene Transcription of Rat Lens Epithelial Cells (RLE cells) in vitro
    亚硒酸钠对大鼠晶体上皮细胞αA晶体蛋白基因转录的影响
短句来源
    CONSTRUCTION OF WISTAR RAT SUCKLING LENS cDNA LIBRARY
    Wistar大鼠乳鼠晶体cDNA文库的构建
短句来源
    Cloning,expression and biological activity identification of Cu-Zu SOD from rat epithelial cells of lens
    大鼠晶体上皮细胞Cu-ZnSOD的分子克隆表达和活性鉴定
短句来源
    Morphometric study on AgNORs of lens epithelial cell by irradiation
    晶体上皮细胞经辐射后AgNORs的形态定量研究
短句来源
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  crystalloid
    THE STUDY ON THE CONFIGURATION OF THE MICROBODY CRYSTALLOID OF STEVIA REBAUDIANA BERTONI
    甜菊叶片细胞微体晶体立体构型研究
短句来源
    Effect of Cold Blood and Crystalloid Cardioplegia on Cardiac Sarcoplasmic Reticulum Ca~( 2+) -ATPase and Ca 2+ Uptake
    冷血及晶体心肌麻痹液与肌浆网钙泵功能改变
短句来源
    Effects of Single Dose Hyperkalemic Crystalloid Cardioplegic Solution on Energy Metabolism and Function of Immature Myocardium
    单次灌注高钾晶体心脏停搏液对未成熟心肌能量代谢和功能的影响
短句来源
    Various crystalloid exists in petioles of four species plants living in the three area (except Prunus persica Linn living in Changchun city).
    在三个地区四种植物叶柄中(除长春的桃),发现有各种形态的晶体存在。
短句来源
    Objective:The aim of this study was to evaluate the myocardial protective efficicacy attained by myocardial temperature during cold blood(CBC) and crystalloid cardioplegia(CC).
    目的:评价冷血心肌麻痹液(CBC)及心肌温度与晶体心肌麻痹液(CC)心肌保护效果的关系。
短句来源
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      crystal
    Using the theory of crystal bases as the main tool, we prove a quantum analogue of Richardson's theorem.
          
    Parameterizing Hecke Algebra Modules: Bernstein-Zelevinsky Multisegments, Kleshchev Multipartitions, and Crystal Graphs
          
    Here, we report using the estrogen receptor that the location of drugs in x-ray crystal structures of the receptors matches closely their predicted spatial locations in the DNA.
          
    The ageing of the reaction mixture reduces the growth rate of mordenite crystal along a-axis and b-axis but hardly influences the growth rate along c-axis.
          
    The growth rate of the mordenite crystal along individual axes increases first and then decreases with increasing concentration of sodium hydroxide.
          
    更多          
      crystals
    Using the path model and the theory of crystals, we generalize the concept of patterns to arbitrary complex semi-simple algebraic groups.
          
    As a result, the boundaries between the surface crystals become a little larger with prolonging the period of ageing time.
          
    With increasing the amount of salt, there was hardly influence on the growth rate along c-axis, whereas an obvious decline was observed in the growth rate along either a-axis or b-axis, which enlarges the boundaries between the surface crystals.
          
    The use of a temperature as high as 473 K produces a membrane composed of bar-like crystals with larger boundaries.
          
    XRD pattern and SEM micrograph showed a good dispersion of ammonium carnallite and anhydrous magnesium chloride crystals with well-distributed big grains, just enough to meet the need for the production of magnesium metal in the electrolysis process.
          
    更多          
      lens
    The system consists of a commercial CCD camera, narrow-band composite filter lens, an image capturing card, an industrial computer, a welding control unit, a GMAW power source, and a worktable.
          
    We studied tissue-specific expression of homeobox genes Pax6, Prox1, and Six3 during regeneration of the retina and lens.
          
    High hybridization signal of all studied genes was revealed in actively proliferating epithelial cells of the native and regenerating lens, while the corneal epithelium demonstrated a lower signal.
          
    After 22.0 weeks of development, Pax6 expression increased in the retina and lens but decreased in the cornea.
          
    Prox1 expression was apparent in the cornea, lens, retina, and eye membranes after 9.5 weeks.
          
    更多          
      crystalloid
    Burn shock fluid resuscitation is currently predominantly performed utilizing crystalloid solutions.
          
    Functional consequences of acute collagen degradation studied in crystalloid perfused rat hearts
          
    Following this in vivo PC protocol, the hearts were removed and perfused on a Langendorff apparatus with crystalloid buffer.
          
    Therefore Biseko?, a serum protein solution, was compared to albumin and crystalloid infusion (Elomel).
          
    The formation rate of H2O2 depended on the difference in crystalloid phase.
          
    更多          


    1. By treatment with sodium sulfite and sodium tetrathionate, the disulfide linkages in theinsulin molecule were split into S-sulfonate groups. From the split products, pure A and B chainswere obtained by ion-exchange chromatography or by zone electrophoresis on cellulose powder. 2. The S-sulfonates of A and B chains were completely inactive in the mouse-convulsion test.After reduction of the S-sulfonate groups by excess thioglycolate to sulfhydryl groups followed byaerobic oxidation at pH 8.5, neither pure...

    1. By treatment with sodium sulfite and sodium tetrathionate, the disulfide linkages in theinsulin molecule were split into S-sulfonate groups. From the split products, pure A and B chainswere obtained by ion-exchange chromatography or by zone electrophoresis on cellulose powder. 2. The S-sulfonates of A and B chains were completely inactive in the mouse-convulsion test.After reduction of the S-sulfonate groups by excess thioglycolate to sulfhydryl groups followed byaerobic oxidation at pH 8.5, neither pure A chain nor pure B chain alone gave active product, butwhen reduced A and B chains were reoxidized together at pH 8.5, considerable insulin activitywas regenerated. The activity recovered was usually about 5-10% of the activity of crystallineinsulin. 3. Regeneration of insulin activity also took place when the reduced form of one of the chainswas incubated with the S-sulfonate of the other chain. 4. Purification of the active, reoxidized product resulted in a crystalline material which had aspecific activity of 18.4 international units/mg in the mouse-convulsion test and possessed the samehypoglycemic activity as insulin. It was indistinguishable in crystal shape from insulin (Fig. 7),and had the same electrophoretic property (Fig. 9) as well as the same R_F values in three solventsystems (Fig. 10) as those of crystalline insulin. 5. By two-dimensional paper electrophoresis-paper chromatography the peptic digests of crystal.line insulin and of this crystalline material gave essentially the same "finger-prints" (Fig. 11).

    (1)用亚硫酸盐及四硫硫酸盐将胰岛素的硫-硫键拆成S-磺酸基后,再经过离子交换或板型电泳分离,可以得到纯的A及B链。(2)用小白鼠惊厥法检查,S-磺酸型A及B链都不具有生物活力。用过量巯基乙酸将纯的A或B链的S-磺酸基还原为巯基,然后分别将还原型的A或B链溶液(pH 8.5)单独在空气中氧化也不产生活力。但将还原型A及B链混合,在同样条件下氧化时,可以获得有活力的产物。活力一般恢复到原活力的5-10%左右。(3)用还原型的A或B链分别与S-磺酸型的B或A链共同保温都可以得到有活力的产物。(4)将恢复活力的重氧化产物进行提纯的结果得到比活力为每毫克18.4国际单位的结晶。这样所获得的晶体其结晶形状、降血糖性质、电泳性质以及在三种溶剂系统中的比移都和结晶胰岛素相同。(5)以上晶体和结晶胰岛素的胃蛋白酶酶解图谱也基本上相同。

    Crystalline needles and spindles of tropomyosins from the smooth muscles of pig stomach, pig urinary bladder, duck and goose gizzard, the striated muscle of crab (Eriocheir sinensis M. Edw.) and the adductor and foot muscles of bivalves (Anodonta pacifica Heude) and of paramyosins from the two last-mentioned muscles were examined under a HU-11 electron microscope. The samples were either stained with phosphotungstic acid or shadow-casted with Pt or Pt-Ir. All of these showed distinct cross striations. The banded...

    Crystalline needles and spindles of tropomyosins from the smooth muscles of pig stomach, pig urinary bladder, duck and goose gizzard, the striated muscle of crab (Eriocheir sinensis M. Edw.) and the adductor and foot muscles of bivalves (Anodonta pacifica Heude) and of paramyosins from the two last-mentioned muscles were examined under a HU-11 electron microscope. The samples were either stained with phosphotungstic acid or shadow-casted with Pt or Pt-Ir. All of these showed distinct cross striations. The banded structure and the appearance of the tapering ends suggested that they were ordered, one-dimensional paracrystalline arrays, not true crystals with threedimensional regularity.The paracrystals of the tropomyosins investigated were all endowed with cross striations with a fundamental repeat period of n×200A, where n=1,2. The 400A period may or may not be accompanied by an intermediate line. These spacings were identical with those of the open-network lattice observed in true crystals of rabbit tropomyosin.The patterns of paracrystals of duck or goose gizzard deserved special attention. In addition to the 400 A and 200 A periods or intraperiod spacings, a prominent 800A (n=4) period was often observed. It seemed not unlikely that 800A corresponded to the particle length of this tropomyosin. Shadow-casted specimens of paracrystals of Anodanta adductor tropomyosin also revealed axial periods of about 800 A.The patterns of the paracrystals of paramyosins obtained from the foot and adductor muscles of Anodonta resembled closely those obtained from the adductor muscles of its marine relatives reported in literature. There were 4 equally-spaced intraperiod lines in a period of about 700 A, the spacing of these lines being 140 A.Obtained in the presence of high salt concentrations, the paracrystalline arrays of the tropomyosins from the adductor and foot muscles of Anodonta differed significantly and interestingly from those obtained conventionally in the presence of low salt concentrations. Although the fundamental repeat of about 400 A remained the same, the intraperiod fine spacings changed now from the 200 A characteristic of tropomyosin to the 140 A characteristic of paramyosin.On the basis of electron microscopic periodicities, it was tentatively suggested that tropomyosin and paramyosin were two different kinds. of proteins. Nevertheless, when they co-existed in one and the same muscle, such as the adductor or foot muscle of Anodonta, the existence of some genetic relationship between the two may have to be reckoned with.

    (一)自猪胃、猪膀胱平滑肌、鵝肫、鴨肫平滑肌、河蟹横紋肌和河蚌閉壳肌及斧足肌制备出原肌球蛋白的針状晶体,自河蚌的两种肌肉制备出副肌球蛋白的針状晶体,分別在电子显微鏡下观察其横紋結构与細节。(二)各种原肌球蛋白的針状晶体与河蚌的副肌球蛋白晶体相类,一般都不是具有三維規律性的真正晶体,而是具有横紋結构和整齐外形的纤維聚集物——类晶体。(三)各种原肌球蛋白类晶体的横紋周期,大都属于n×200A(n=1,2,…)数系,周期或为400A、或为200A。其具有400A周期的,有时伴有200A的細节。这些共同的特征与免原肌球蛋白真正晶体的三維网状周期相同。(四)鴨肫和鹅肫原肌球蛋白的类晶体中,除了400A,200A的横紋外,还出現800A(n=4)的周期,显示其分子长度可能至少不低于800A。(五)河蚌斧足肌和閉壳肌副肌球蛋白的类晶体,同海蚌一样,都具有周期約700A的横紋結构。条件适宜时,还可以在每一周期之間观察到4条横紋,細节間距为140A。(六)河蚌閉壳肌原肌球蛋白的类晶体中,也有接近8...

    (一)自猪胃、猪膀胱平滑肌、鵝肫、鴨肫平滑肌、河蟹横紋肌和河蚌閉壳肌及斧足肌制备出原肌球蛋白的針状晶体,自河蚌的两种肌肉制备出副肌球蛋白的針状晶体,分別在电子显微鏡下观察其横紋結构与細节。(二)各种原肌球蛋白的針状晶体与河蚌的副肌球蛋白晶体相类,一般都不是具有三維規律性的真正晶体,而是具有横紋結构和整齐外形的纤維聚集物——类晶体。(三)各种原肌球蛋白类晶体的横紋周期,大都属于n×200A(n=1,2,…)数系,周期或为400A、或为200A。其具有400A周期的,有时伴有200A的細节。这些共同的特征与免原肌球蛋白真正晶体的三維网状周期相同。(四)鴨肫和鹅肫原肌球蛋白的类晶体中,除了400A,200A的横紋外,还出現800A(n=4)的周期,显示其分子长度可能至少不低于800A。(五)河蚌斧足肌和閉壳肌副肌球蛋白的类晶体,同海蚌一样,都具有周期約700A的横紋結构。条件适宜时,还可以在每一周期之間观察到4条横紋,細节間距为140A。(六)河蚌閉壳肌原肌球蛋白的类晶体中,也有接近800A的周期。(七)在高盐条件下所获得的河蚌斧足肌和閉壳肌原肌球蛋白的类晶体,与上述一般低盐条件下的类晶体不同,細节間距与副肌球蛋白的相同,为140A,而非200A。(八)根据电子显微鏡下观察到的类晶体横紋周期和細节間距,可以初步推論:一般而言,原肌球蛋白和副肌球蛋白是两类而非一类的蛋白貭。但在两种蛋白貭共存的同一肌肉,如河蚌閉壳肌或斧足肌之中,它們之間还可能存在着一定的亲属关系。

    Crystalline spindles of the tropomyosin of the body muscle of the prawn, Penaeus orientalis, were examined under a HU-11 electron microscope. The samples were prepared by the different techniques of staining, replica and ultra-thin sectioning. The crystals were endowed with cross striations with a repeat period of ca. 400 A characteristic of tropomyosins. In addition, there were two equally-spaced intraperiod lines so that the fundamental repeat was about 140 A, identical with that of paramyosin. We are confronted,...

    Crystalline spindles of the tropomyosin of the body muscle of the prawn, Penaeus orientalis, were examined under a HU-11 electron microscope. The samples were prepared by the different techniques of staining, replica and ultra-thin sectioning. The crystals were endowed with cross striations with a repeat period of ca. 400 A characteristic of tropomyosins. In addition, there were two equally-spaced intraperiod lines so that the fundamental repeat was about 140 A, identical with that of paramyosin. We are confronted, therefore, with yet another case of the type of band structure intermediary between tropomyosin and paramyosin. The other two cases~([11]) were the tropomyosins of the adductor and foot muscles of Anodonta Pacifica, Heude. It may be recalled that the salting-out range of this prawn tropomyosin was 15-30% ammonium sulphate saturation~([4]), like an "insoluble tropomyosin".In the absence of salt ions, the particles of Penaeus tropomyosin polymerized in solutions to give submicroscopic long fibrils. The fibrils were thick in the middle, with tapering ends. They showed distinct cross striations with a repeat period of 140 A.When the crystals were fixed and stained with osmic acid for several days, a dotted band structure was observed which was reminiscent of the lattice point structure observed by Schmitt and his co-workers~([8,9]) in paramyosin fibrils; but the repeat period was 400 A.Our results are consistant with the idea~([3,11]) that the band structures shown by tropomyosin, paramyosin and light meromyosin are different and characteristic of the individual proteins and are therefore one means of fingerprinting these proteins. But our results, especially the finding of the "intermediary" type of band structure in Penaeus and Anodonta tropomyosins, suggest yet another aspect of the problem, i. e.,the inherent structural relationship between these proteins.

    (1)利用锇酸、磷钨酸染色,复型和超薄切片等技术在电子显微镜下观察了对虾原肌球蛋白长纺锤形晶体的横纹结构。对虾原肌球蛋白在溶解度上与副肌球蛋白接近。(2)对虾原肌球蛋白晶体的横纹周期约400A,与一般的原肌球蛋白相同;而其横纹细节的间距为140A,却与副肌球蛋白相类。因此,对虾原肌球蛋白一如河蚌闭壳肌或斧足肌原肌球蛋白,构成两类蛋白质之同的“中间”类型。(3)在无盐的水溶液中,对虾原肌球蛋白聚合所形成的亚显微纤维,也具有横纹结构,其同距也是140A。(4)讨论了原肌球蛋白,副肌球蛋白和轻酶解肌球蛋白晶体横纹结构的特征与相同之处以及它们与分子结构可能有的内在联系。

     
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