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A new method for the biological assay of vegetable purgatives based on the numbers of wet faeces excreted by groups of mice after dosing is described. The relation of the number of wet faeces per group of mice to the logarithm of the dose was found to be linear. A cage has been specially designed for this assay and it has been found advantageous to incorporate a definite proportion of water in the diet during test. 40 mice divided equally into 4 groups were used in each assay. 2 groups received the standard...

A new method for the biological assay of vegetable purgatives based on the numbers of wet faeces excreted by groups of mice after dosing is described. The relation of the number of wet faeces per group of mice to the logarithm of the dose was found to be linear. A cage has been specially designed for this assay and it has been found advantageous to incorporate a definite proportion of water in the diet during test. 40 mice divided equally into 4 groups were used in each assay. 2 groups received the standard preparation and the other 2 groups received the test preparation. The standard deviation of a single determination based on 9 such assays was estimated to be 15.7 per cent. For rhubarb and its preparations, a“6-point”assay is advocated. With suitable restric-tion in the design of the assay, it is possible to calculate the potency by simple methods. Powdered crude drugs are used as laboratory standards in the assay of senna and rhubarb. The doses of cascara bark required to produce distinctive responses are too inconveniently large to be administered in suspension. It was found possible to use a potent extract of cascara in place of the powdered bark as a laboratory standard. Examples of the assay and the subsequent calculations are given. The method described is not only convenient in use but also gives a comparatively high degree of accuracy. The method has been successfully applied to senna leaf, senna fruit, rhubarb, cascara sagrada and extracts and commercial preparations made from these drugs, pure glycosides(sennosides A and B) and pure anthracene compounds(aloe emodin and aloe-emodine anthranol).

(1)本文详述植物性泻药的一种新的生物测定法,此法係用小白鼠在服药后所排出的濕粪数为基础,以定泻药的效价。(2)小白鼠服药後所排出的湿粪数与剂量指数的关係经证明为一直线。(3)本法所用鼠笼係特别设计,并证明在饲料内加入一定量的饮水,具有多种优点。(4)番泻效价的测定,每次用小白鼠4,每10只。2给与标准品,另外2给与试验品。根据9次试验的结果,试验的标准差是15.7%.(5)大黄效价的测定,以采用“6点”法为宜,即每次试验用小白鼠6,3给与标准品,另外3给与试验品,所得的准确度与上述相仿。波希鼠李皮浸膏的效价测定,则用“4点”法或“6点”法均可。(6)所有试验一律采用粉状生药为实验室的比较标准,但是波希鼠李皮的效价太低,它的粉末不适於作为标准之用,可用波希鼠李皮乾浸膏来代替,作为比较标准。(7)本文对於效价测定及其计算方法都举例说明,并介绍了比较常法为简捷的相关效价计算法。(8)本法不但方便易行,而且有相当高的准确度;曾用在番泻、大黄、波希鼠李皮及其制剂,以及一些纯粹的蒽醌衍生物的效价测定,都获得了满意的结果。

The present investigation aims at introducing the distribution of medicinal plants in Northeastern China and makes an attempt to offer reference to the study of chinese medicine. About 2000 species of plants have been collected in this region from 1938 to 1955. Among these plants, 300-400 are of medicinal uses, of them 156 are famous indigenous drugs, such as: Panax ginseng C. A. Meyer, Ephedra distachya L., Glycyrrhiza uralensis Fischer, Gentiana scabra Bge., Schizandra chinensis Bail., Asarum sp., Aconitum...

The present investigation aims at introducing the distribution of medicinal plants in Northeastern China and makes an attempt to offer reference to the study of chinese medicine. About 2000 species of plants have been collected in this region from 1938 to 1955. Among these plants, 300-400 are of medicinal uses, of them 156 are famous indigenous drugs, such as: Panax ginseng C. A. Meyer, Ephedra distachya L., Glycyrrhiza uralensis Fischer, Gentiana scabra Bge., Schizandra chinensis Bail., Asarum sp., Aconitum spp., etc.. They are either very abundant in quantity or supreme in quality. Some of them are being cultivated in large scale. Investigation shows that the resource of medicinal plants in this region is very rich and requires further investigation.

本文目的旨在报導关于中國东北藥用植物分布情况,作为研究中藥的参考。作者以及在本組工作的前人从1938到1955年,經过多次調查采集獲得了約2000种本地植物,其中包括有300—400种藥用植物,并且还有156种著名的中藥例如:人参、甘帅、麻黄、細辛、烏头、龍胆及紫等。这些藥物都是量多而質优的。其中还有些已經在大量栽培。东北藥用植物資源非常丰富,尚有待于進一步地調查和研究。

Experiments are performed to use a brand of Chinese filter paper in paper chromatographic analysis. The paper used is the "fine" grade filter paper manufactured by the First Eastern Factory, Fou-Shun. It appears that the filter paper, after adequate treatment, may be used in paper chromatographic work. Treatment essentially consists of washing with dilute hydrochloric acid and distilled water and ascending with suitable solvents of the particular cation-groups. In this experiment, the solvents used for chromatographic...

Experiments are performed to use a brand of Chinese filter paper in paper chromatographic analysis. The paper used is the "fine" grade filter paper manufactured by the First Eastern Factory, Fou-Shun. It appears that the filter paper, after adequate treatment, may be used in paper chromatographic work. Treatment essentially consists of washing with dilute hydrochloric acid and distilled water and ascending with suitable solvents of the particular cation-groups. In this experiment, the solvents used for chromatographic analysis of aluminium, silver and arsenic groups were modified from that used by F. H. Burstall and others. The results are summarized as follows:Group I R_f: K 0.45, Na 0.56, Mg 0.71Group Ⅱ R_f: Ba 0.45, Sr 0.53, Ca 0.61Group Ⅲa R_f: Al 0.02, Zn 0.73Group Ⅲb R_f: Fe 0.99, Co 0.44, Mn 0.30, Ni 0.00Group Ⅳa R_f: Ag 0.16, Pb 0.00, Hg 0.80Group Ⅳb R_f: Pb 0.02, Cu 0.30, Bi 0.67, Cd 0.82, Hg 0.90Group V R_f: As 0.79, Sb 0.93, Sn 0.95Besides, we have found a number of common reagents which can be used to develop the cations on such kind of filter paper. After chromatographic separation, their sensitivity is determined. The R_f of both the ascending and descending processes has very close values. When temperature varies within 5°C, the R_f values change very little.

1.国产抚顺东一廠细号濾纸对无机阳离子層析的效果不低於进口滤纸。为了適應该纸的性質,铝族、银族、砷族所用溶剂曾略加修改。并找出该纸所含杂質较多,使用前须先用该溶剂上昇一次,同时又可消除鬼斑(ghost spot)。 2.在此国产濾纸上较全面地整理了各分析离子層析的条件,以便於未知物在此纸上进行系统層析及检验。并對層析後的離子进行了靈敏度的测定。 3.在国产纸上層析无机陽離子时,温度以及上昇法與下降法對R_f值之影响不大。

 
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