The level of CD44v6 expressions in PBMC and cancerous tissue in patients with gastric cancer of Ⅲ ± Ⅳ stage(12.56%±6.97% and 3.91%±0.87% respectively) was significantly higher than that of Ⅰ ±Ⅱ stage(6.89%±3.37% and 2.86%±0.65% respectively,both P<0.05).
In blood sample from 66 patients with gastric cancer, positive expression rate of CK19 was 53.1% (35/66), positive expression of CK20 was 56.1% (37/66), positive expression of CK19 and CK20 was 46.9% (31/66).
However,there was significant difference between the serum level of VEGF-A in16patients with gastric cancer prior and three weeks after operation(328.4pg/ml±141.7pg/ml vs.103.5pg/ml±52.2pg/ml,P<0.0001).
(2)The activity of CD4~+CD25~+ and CD4~+CD25~-T cells in healthy control and the gastric cancer patients after sorting were 98.52%±0.72% and 97.80%±0.95%. There was no significantly difference between the two groups (P>0.05).
Methods The levels of IL 2 , sIL 2R, PGE2 and the expression of CD25 in the peripheral blood in 50 patients with gastric carcinoma were detected by ELISA, 125I RIA and immunofluoresence methods respectively.
Reversal of multidrug resistance of gastric cancer cells by downregulation of CIAPIN1 with CIAPIN1 siRNA
The overexpression of a new cytokine-induced apoptosis inhibitor 1 (CIAPIN1) gene has been shown previously to promote a multidrug resistant phenotype in gastric cancer cells through the upregulation of MDR1 and MRP1.
CIAPIN1 siRNA could significantly downregulate the expression of Bcl-2, and upregulate the expression of Bax, but does not alter the expression of PTEN in gastric cancer cells.
These observations suggested that the siRNA constructs of CIAPIN1 we obtained could effectively downregulate the expression of CIAPIN1 and reverse the resistant phenotype of gastric cancer cells.
Further study of the biological functions of CIAPIN1 may be helpful for understanding the mechanisms of multidrug resistance of gastric cancer and in developing possible strategies to treat gastric cancer.
Effect of perioperative autologous versus allogeneic blood transfusion on the immune system in gastric cancer patients
No significant differences in the RFLPs were observed between normal individuals and gastric cancer patients.
Research on serum levels of retinol, α-tocopherol β-carotene, and 12 elements in gastric dysplasia and gastric cancer patients
The results showed that β-carotene and Se, Co were lower in gastric cancer patients than that in gastric dysplasia patients or in normal subjects, Ni and Cr levels were lower and Mn, Ca and Cd were higher in gastric cancer than in dysplasia patients.
Zn, Fe, Cr, Cd were lower and Mn, Ca, Mg, Mo levels were higher in gastric cancer patients than in normal.