In order to compare with the adsorbing effect of the two kinds of materials, Ag~+, [Ag(NH_3)_2]~+, [Ag(S_2O_3)_2]~(3-) were chosen as adsorbents, and diatomite, bentonite, zeolite, and microzyme were chosen as adsorates.
In the conditions of 5minutes, pH5.41, initial concentration of Ag~+ of 216mg/L, biomass of 16.7g/L, room temperature, the removal rate that microzyme adsorbed Ag~+ was up to 92.67%, and the adsorbing quantity was 15.0518mg/g.
Optimumconditions were saccharomycete and lactic acid bacteria in proportion of two to three, fermented time 2. 5 h at 37 ℃ or 43 ℃ individed Stage fermentation, addition of 6% sugar and fermented milk with a titratable acidity of 84 °T.
The amount of Saccharomycete and that of Lactobacillus in treatment with addition of 1.0% CGF derived from the fourth extraction method after 24h were 14.98 and 2.2 times respedively to that of control treatment.
An improved yeast two-hybrid approach for detection of interacting proteins
Yeast two-hybrid approach is popularly used nowadays as an important technical method in the field of studying protein-protein interactions.
Although yeast two-hybrid system is obviously advantageous in searching interacting proteins and setting up the network of proteins interaction, not all of proteins can use routine yeast two-hybrid method to search interacting proteins.
Cloning and expressing a recombinant human tissue inhibitor of metalloproteinase-2 (TIMP-2) in methylotrophic yeast Pichia pasto
The verified gene of TIMP-2 was cloned to the Escherichia coli-yeast shuttle vector of pPIC9 to construct a recombinant plasmid pPIC9-T2.