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福尔根
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  feulgen
     Analysis of cellular DNA content in colorectal carcinoma was performed by combination of 3H-TdR autoradiography and Feulgen cytophotometry.
     进行~3H—TdR放射自显影术和福尔根细胞光度法分析大肠癌的细胞DNA含量。
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     By using the combination of ~3H-TdR autoradiography and Feulgen cytophotometry to measure the cellular DNA content of unlabelled cells (G1), it is shown that the DNA amount in the experimental group is lower than that in the control group.
     同时应用~3H-TdR放射自显影和福尔根细胞光度法测定未标记细胞(G_1期)DNA含量,结果显示实验组比对照组降低了。
短句来源
     3HTdR-autoradiography and Feulgen reaction were carried out successively in smear preparations of single cells. The proportion of proliferating cells ( S, G2 ) labeled by silver grains was counted, then Feulgen-density of unlabeled cells (G1 ) in the same slide was determined cytophotometrically.
     将单个细胞涂片连续进行~3HTdR-放射自显术和福尔根反应,先计算银粒标记的增殖细胞(S,G_2)的比例,再用细胞光度法测量未标记细胞(GI)的福尔根光密度。
短句来源
     Methods:The Feulgen squash technique was used.
     方法采用福尔根压片法。
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  相似匹配句对
     Root
    
短句来源
     Methods:The Feulgen squash technique was used.
     方法采用福尔压片法。
短句来源
     It is the dried root of plant Ficus hirtaVahI.
     的干燥
短句来源
     Analysis of cellular DNA content in colorectal carcinoma was performed by combination of 3H-TdR autoradiography and Feulgen cytophotometry.
     进行~3H—TdR放射自显影术和福尔细胞光度法分析大肠癌的细胞DNA含量。
短句来源
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  feulgen
Cytophotometric measurements of Feulgen-DNA were carried out in the nuclei of embryo sac cells of Haemanthus albiflos and Ornithogalum caudatum.
      
Cellular DNA and AgNOR protein contents were evaluated byautomatic image analysis in tissue sections stained by combined Feulgen-AgNOR staining method in 9 normal colonic mucosae, 45 colorectal adenomas and 27 adenocarcinomas.
      
Methods: The routine paraffin slides were cut, stained with HE, immunochemically by ABC methodusing the and stained by Feulgen method.
      
The pancreatic tissues were taken for HE and Feulgen staining.
      
At 20 °C, the retinal cells were withdrawn every 2 h within 0 to 28 h after death and stained with Feulgen-Vans.
      
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A multi-methodological study was performed on the preservation of cellular and subcellular structures and nucleic acid of an ancient cadaver of Mawangtui Tomb No. I. It was found by microscopy that chondrocytes of the cadaver were the most perfectly preserved cells. Some of them showed nearly normal morphology, their cytoplasm appeared homogeneous or vacuolated, with a distinct nucleus of loose architecture. However, under electron-microscopy, the intracellular ultrastructures were found to be mostly destroyed,...

A multi-methodological study was performed on the preservation of cellular and subcellular structures and nucleic acid of an ancient cadaver of Mawangtui Tomb No. I. It was found by microscopy that chondrocytes of the cadaver were the most perfectly preserved cells. Some of them showed nearly normal morphology, their cytoplasm appeared homogeneous or vacuolated, with a distinct nucleus of loose architecture. However, under electron-microscopy, the intracellular ultrastructures were found to be mostly destroyed, leaving only a few structures which resembled residual endoplasmic reticulum and nuclear membrane fragments. By the methods of the histochemical Feulgen reaction and cytochemical photometry and, in addition, biochemical extraction, several lines of evidence were obtained indicating that macromolecular nucleic acids were present in these chondrocytes, including both deoxyribo- and ribonucleic acids. Apparent degradation of these nucleic acids was also evident.Accompanying the preserved cells numerous bacterial spores with very perfect structure were seen in the tissues of the cadaver.In light of the above observations, the preservation of cell structure was discussed. It was concluded that the state of preservation of the cadaver was fairly good at the cellular level, especially in the case of chondrcoytes; but even in these cells, nothing typical of normal cellular fine ultrastructures were present.

本文对马王堆一号汉墓古尸进行了细胞、超微结构以及核酸保存状态的研究。显微镜的观察发现软骨细胞保存最为完整。有些软骨细胞接近正常形态,细胞界限清楚,并含有比较完好、结构疏松的核。但在电子显微镜下观察,这些细胞的超微结构已大部崩解,只见到一些类似内质网的残迹和核膜的断片。组织化学的福尔根反应,细胞化学的紫外吸收和生化的抽提鉴定,都一致证明软骨细胞中尚保存着一定大分子性质的核酸,包含脱氧核糖核酸和核糖核酸。这些核酸显然已遭受较大程度的降解和破坏。古尸组织中伴同细胞,保存着非常完整的细菌芽孢。根据这些观察,讨论了细胞的保存程度,认为古尸的保存已达到了细胞水平。

3HTdR-autoradiography and Feulgen reaction were carried out successively in smear preparations of single cells. The proportion of proliferating cells ( S, G2 ) labeled by silver grains was counted, then Feulgen-density of unlabeled cells (G1 ) in the same slide was determined cytophotometrically. Although the labeling index of the active lymphocytes induced by PHA in vitro reaches 30.17%, isotope does not seem to be incorporated into the cells of juvenile polyps, and only small number of cells in various types...

3HTdR-autoradiography and Feulgen reaction were carried out successively in smear preparations of single cells. The proportion of proliferating cells ( S, G2 ) labeled by silver grains was counted, then Feulgen-density of unlabeled cells (G1 ) in the same slide was determined cytophotometrically. Although the labeling index of the active lymphocytes induced by PHA in vitro reaches 30.17%, isotope does not seem to be incorporated into the cells of juvenile polyps, and only small number of cells in various types of adenomatous polyps and adenomas were labeled by silver grains. The DNA content ( 24.06 ) of lymphocytes in prometaphase ( 4C ) is used as a reference standard, DNA indices ( DI ) were calculated from the ratio of the DNA content in the analyzed cells to normal diploid value( 12.03). The cellular DNA content in 5 cases of juvenile polyps is mainly within normal diploid range ( mean 12.06, DI=1.00) , however, hyperdiploid abnormality prevails in the 5 cases of abnomatous polyps and adenomas(mean 14.36, DI=1.19). The difference of the DNA value between two groups of polyps is statistically significant. Because the former group has no apparent: relation to adenocarcinoma, whereas the latter group frequently tends to undergo malignant change, these data led us to speculate that abnormal DMA content or aneuploidy may be considered as a certain sign of malignancy.

将单个细胞涂片连续进行~3HTdR-放射自显术和福尔根反应,先计算银粒标记的增殖细胞(S,G_2)的比例,再用细胞光度法测量未标记细胞(GI)的福尔根光密度。虽然受PHA刺激的活性淋巴细胞的标记指数达30.17%,但幼年性息肉不能参入同位素,各种腺瘤性息肉和腺瘤中只有少数标记细胞。以前中期(4C)淋巴细胞的相对DNA含量(24.06±2.67)为衡量标准,在与二倍体值(2C,12.03)比较后算出测量细胞的DI。5例幼年性息肉细胞的DNA含量为二倍体值(平均12.06,DT=1.00),5例腺瘤性息肉和腺瘤为超二倍体值(平均14.36,DI=1.19)。两类息肉的DNA含量差异显著。鉴于前者与腺癌无关,后者易于癌变。因此,不正常DNA含量或非整倍体性可能是细胞癌变的征兆。

Short-term culture of PHA(phytohemag-glutinin) stimulated human lymphocyte iswidely used for analysis of cell cycle kineticsand cytogenetics.The same technique also haswidespread application to detect chromosomedamage caused by exogenous mutagen and im-mune response of blood.A deep understand-ing of lymphocyte proliferation is essentialfor these studies.In present experiment,acombination of radioautography to determinewhen a cell synthesized DNA,and cytopho-tometry to measure the cellular DNA content,was carried...

Short-term culture of PHA(phytohemag-glutinin) stimulated human lymphocyte iswidely used for analysis of cell cycle kineticsand cytogenetics.The same technique also haswidespread application to detect chromosomedamage caused by exogenous mutagen and im-mune response of blood.A deep understand-ing of lymphocyte proliferation is essentialfor these studies.In present experiment,acombination of radioautography to determinewhen a cell synthesized DNA,and cytopho-tometry to measure the cellular DNA content,was carried out to characterize the blastogenicresponse of lymphocyte. Fresh human peripheral blood was addedto RPMI 1640 medium containing fetal calfserum and PHA.When cultured at 37℃ for72 hours,lymphocytes are stimulated andtransform into blastic cells which then proli-ferate.Before the cells were harvested,0.5μCi/ml 3~H-TdR were offered into cultures.After removal of red cells by hypotonic shocktreatment,the remaining lymphocytes werecollected and smeared.Radioautographyic pro-cedures and Feulgen reaction were carried onthe preparations sequently.The percentage oflymphocytes labeled by silver grains (S) firstcounted,then Feulgen density of unlabeledcells (G_1) random selected in the same slidewas measured cytophotometrically.In orderto check the technical error in various prepa-rations,chick erythrocytes were added on eachslide to provide an internal staining standard.The relative DNA content (24.06) of prome-taphase (4 C) of lymphocyte was used as areference standard for ploidy.Six groups of blood cultures were treated with isotope for 0,0.5,2,4,7 and 17 hours,the labellingindexes of activated lymphocytes in respectivepreparations have 0%,1.90%,9.15%,13.40%,15.01% and 30.13%.In addition,Feulgen density of unlabeled cells in same sli-des reach 15.42 (DI=1.28),15.45 (DI=1.28),14.88(DI=1.24),13.77(DI=1.14),13.20(DI=1.10) and 12.94(DI=1.08) re-spectively.Even treated with isotope for onecell generation time (17 h),there are alwaysa considerable number of unlabeled lympho-cytes to exist in 2 C —4 C DNA range.Obvious-ly,these intermediate DNA value are notdue to nuclei being in S phase at the time.It would like to propose that DNA synthesisin S phase of lymphocytes may be not contin-uous. On the other hand,attempt was made tocorrelate DNA synthesis with DNA contentat single cell level.141 labeled lymphocyteswere selected at random on radioautographicslides,their coordination were recorded inmicrophotographic films for relocation.First,DNA newly synthesized in individual cell inS phase was determined by quantitative 3~H-TdR-radioautography.Optical density of silvergrains of labeled cells (S) were measured intransmitted light at 546 nm (mean 28.82).Secondly,after dissolving silver grains in so-lutions of potassium ferric cyanide and sodiumthiosulfate,the same slides were Feulgen stain-ed.DNA content of individual preselectednucleus was measured at 546 nm (mean 16.27,DI=1.35).We found that the labeled cellsdisplay a great difference in the number ofsilver grains,these cells always possess 2C—4C DNA value.Some concomittant increaseis also observed in the mean value of DNAsynthesis during S phase.These findings me-rit further attention.

体外培养受PHA刺激的人淋巴细胞经~3H-TdR参入0、0.5、2、4、7和17小时后,放射自显术表明标记指数为0%、1.90%、9.15%、13.14%、15.01%和30.13%。未标记细胞的福尔根光密度为15.42、15.45、14.88、13.77、13.20和12.94。DNA分布直方图显示部分未标记细胞介于2C—4C,表明S期细胞的DNA合成可能是不连续的。对同位素参入17小时的标记细胞连续进行两项测量表明,这些细胞的银粒光密度相差悬殊,但其DNA含量均为2C—4C。细胞的DNA合成率随其DNA含量而增高。

 
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