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过滤灭菌
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  “过滤灭菌”译为未确定词的双语例句
     1 (1.54%), 4 (20.00%) and 3 (42.86%) disease resistant somaclonal variants were obtained at concentration of 25%, 50% and 75% fungal culture filtrate as selective agents, respectively, with the total number of 8 plants resistant to P. capsici.
     利用过滤灭菌方式加入选择性培养基的处理中,25%、50%和75%的辣椒疫霉培养滤液分别获得1株、4株和3株抗病株,分别占各处理再生植株总数的1.54%、20.00%和42.86%,共获得8株,占该组处理再生植株总数的8.70%。
短句来源
     Fermentation liquor of Dyst-1 and MYNS-1 treaded individually through autoclaving and filtering by germproofing filter indicates inhibiting effect to selected plant with pathogenic fungi, especially Sclerotinia sclerotiorum .
     Dyst-1和MYNS-1的发酵液通过高压湿热灭菌和细菌过滤器过滤灭菌后,分别对所选用的几株植物病原真菌表现出抑制作用,特别是对油菜种传病害菌核病病菌。
短句来源
     2. Decoction prepared: YXS was prepared and preserved in 0℃ after being poached, alcoholized and settled, being concentrated to 2g/ml and filtrated, and being got ride of bacteria.
     2.药液制备:眼血散复方经水煮醇沉,浓缩至2g/ml,过滤,灭菌,0℃保存备用。
短句来源
     A better plating efficiency was obtained when the stariliization time was shortened to 5 min,at 15 (?) bs/cm~(?) .
     常规的高压灭菌最不利于细胞生长,在15磅/cm~2灭菌5分钟效果较好,过滤灭菌效果最好。
短句来源
     Effects of AC and anther density on pollen embryo and callus production and green plant regeneration were studied in a filter-sterilized, liquid-medium culture system.
     在过滤灭菌的液体培养体系中研究了甘露醇预培养和诱导培养过程中活性碳和花药密度对大麦花培效率的影响。
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  相似匹配句对
     sterilization and packing.
     灭菌、包装。
短句来源
     Sterilization of Medical Devices
     医疗器械灭菌
短句来源
     Collaborative Filtering
     信息协同过滤
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     Filter dewatering of hydraulic oil
     液压油过滤脱水
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  filter sterilization
Tests were performed with resting cells of Escherichia coli, Staphylococous aureus and Serratia marinorubra, inoculated in freshly sampled sea water used raw or after filter sterilization.
      
The coral antibacterial activity, referred to as CAA, was retained following filter sterilization and storage at -20°C.
      
Medium sterilization techniques (autoclaving, filter sterilization and separate sterilization of medium components), combined with preculture exposure to activated charcoal (AC) were evaluated for effects on maize anther culture response.
      
Production of alcohol from sugar beet molasses without heat or filter sterilization
      
tumefaciens virulent strain C 58 with carrot cells and removing the bacteria and carrot cells using filter sterilization.
      
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We have found that the inductibn frequency of calli from rice anthers could be increased remarkably by anther float culture,but the differentiation frequency of calli was rather low.Various media (five induction media and four differentiation media) were tested for the.increase of the diffeTeritiation frequency.Oryza sativa subsp.keng was used in the experiment.,The anthers with the microspores at late uninucleate stage were pretreated at about 10 C for 10 days before inoculation!i Induction media:I.N6+2,4-D...

We have found that the inductibn frequency of calli from rice anthers could be increased remarkably by anther float culture,but the differentiation frequency of calli was rather low.Various media (five induction media and four differentiation media) were tested for the.increase of the diffeTeritiation frequency.Oryza sativa subsp.keng was used in the experiment.,The anthers with the microspores at late uninucleate stage were pretreated at about 10 C for 10 days before inoculation!i Induction media:I.N6+2,4-D 2mg/l+LH 500 mg/1 + Sucrose 3%;II.N6+2,4-P 2mg/lTfLH 500 mg/1+Sucrose 6%;III.N6+2,4-D2mg/l+Serine 50 mg/l+ Arginine 50 mg/1+Glutamic acid 50 mg/l+As-paragine 50 mg/1+Sucrose 3%;IV.20% Potato extract +KNO3 1500 mg/l+Ca(NO3)? 4H2O 100 mg/1+Fe-EDTA (N6)+Vitamin B1 1mg/1+2,4-D 2mg/1+Sucrose 3%IV-1 Sterilized- by filtrationIV-2.Sterilized by autoclaveV.II+Agar 0.57% (control).Differentiation media:A.MS+KT+ 2mg/l-(-IAA,1mg/1+ Sucrose 3%+Agar 0,57%;B.A + Vitamin B1 1 mg/1;C.MS + 6-BA 2 mg/1+IAA 1 mg/1+Sucrose 3%+Agar :0.57%;D.A + Serine 50 mg/1+Glutamic acid 50 mg/1+Argirane 50 mg/1+Asparagine 50 mg/1.The main results are as follows:(1)The induction media were more influential than differentiation media in deter-ming the differentiation ability of clalli.The best result of differentiation of calli was obtained from the potato medium sterilized by filtration.Its differentiation frequency of green plantlets attain to 50%,better tnan the control.But the frequency was slightly lower when the potato imedium sterilized.by autoclave was uhed.The effects of the differentiation media on differentiation of calli were irregular.(2)The calli floating on the surface of liquid media possessed a higher ability for differentiation than the calli sank to the bottom of liquid media.(3)The earlier the calli were transferred to differentiation media,the higher the differentiation frequencies of calli could be obtained.When the calli were transferred directly from liquid media to differentiation agar media,its differentiation frequencies were higher than their being first proliferated on agar media and then transferred to differentiation agar media.

水稻花药液体漂浮培养能大幅度提高愈伤组织诱导频率,但这些愈伤组织的分化能力很低。为了提高液体培养下愈伤组织的分化频率,试验了5种诱导培养基和4种分化培养基。结果表明,诱导培养基对愈伤组织分化能力的高低起主要作用,其中以过滤灭菌的马铃薯提取液培养基的效果最好,绿苗分化率可高达50%;分化培养基对愈伤组织分化频率的影响较小,且不甚规律。浮在液面上的愈伤组织比沉在培养液底部的愈伤组织有较高的分化能力。愈伤组织转移时间的早晚对分化频率也有很大影响。

In order to determine the extent and degree of chloroquine resistance of falciparum malaria in our country, we have prepared both the drug-coated microplates and freeze-dried medium for the in vitro microtechnique. Each microplate has 40 wells, four horizontal rows with 10 wells each, wells 1 and 10 were untreated to serve as controls and wells 2-9 coated with 1.0, 2.0, 4.0, 5.7, 8.0, 16.0, 32.0 and 64.0 pmol of chloroquine diphosphate. The freeze-dried medium contained RPMI 1640, HEPES buffer and 15% type AB...

In order to determine the extent and degree of chloroquine resistance of falciparum malaria in our country, we have prepared both the drug-coated microplates and freeze-dried medium for the in vitro microtechnique. Each microplate has 40 wells, four horizontal rows with 10 wells each, wells 1 and 10 were untreated to serve as controls and wells 2-9 coated with 1.0, 2.0, 4.0, 5.7, 8.0, 16.0, 32.0 and 64.0 pmol of chloroquine diphosphate. The freeze-dried medium contained RPMI 1640, HEPES buffer and 15% type AB human serum. Prior to use, it was stored under refrigeration. 0.9ml of 0.21% sodium bicarbonate solution was injected into a vial with freeze-dried medium and the latter dissolved by shaking, and then 0.1ml of patient's blood added, from which an aliquot of 50μl of the mixture being placed in each well. P. falciparum kept in continuous culture for more than a year was used for laboratory assessment of reliability of the freeze-dried medium and microplates. The results obtained showed that the freeze-dried medium stored for one year at 4℃ could still support the growth of malaria parasites while those at 36℃ for 5 days and at 26℃ for 10 days failed. Field investigation was carried out in some parts of Hainan Island, South Yunnan, west Guangxi, South Guizhou and East Anhui. The results showed that the home-made plates were equally effective as those provided by WHO. The application of the freeze-dried medium in the assessment of drug-resistant, malaria furnished the test with a good readability and proved to be suitable for field use. It is clear that the home-made freeze-dried medium might be an important tool in the surveillance of chloroquine resistant malaria.(This investigation received the financial support of the UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases, SWG on the Chemotherapy of Malaria, Project ID No:800214)

体外微量法测定恶性疟原虫对氯喹的敏感性,具有简便、快速和准确等优点,但要有预制的涂氯喳板及培养基才能用于现场调查。世界卫生组织自1979年起试制这种板和冰冻干燥培养基,但后者至今尚无供应。 为了尽快摸清我国抗氯喹恶性疟的分布情况,我们于1979年底仿制涂氯喹板获得初步成功后,1980年又着手研制冻干培养基,几经改进,制成的冻干培养基在4℃保存下有效期已达1年以上,可在加液后立即使用,不需过滤灭菌等手续,所制涂氯喹板的药效曲线与世界卫生组织的完全一致。且增加了一个对照井及一个药物剂量,有利于预先观察疟原虫生长情况及测定较高抗性的病例。1981年7~10月间经粤、桂、滇、黔、皖等省区试用,效果满意。

We determined the osmotic values, pH values of various concentrated medium prior and post autoclaving and the time course of these changes, and studied the condition of polyribosomes by density gradient centrifugation, the gel electrophoretic pattern of soluble proteins and the pollen androgenesis after concentrated medium pretreatments. The results showed that the alteration of polyribosomes was related with the pH values of medium after autoclaving, the alteration of soluble protein patterns was related rather...

We determined the osmotic values, pH values of various concentrated medium prior and post autoclaving and the time course of these changes, and studied the condition of polyribosomes by density gradient centrifugation, the gel electrophoretic pattern of soluble proteins and the pollen androgenesis after concentrated medium pretreatments. The results showed that the alteration of polyribosomes was related with the pH values of medium after autoclaving, the alteration of soluble protein patterns was related rather with the osmotic values post autoclaving, and the condition of androgenesis was related with both pH and osmotic values, but with pH more closely.

测定了多种高浓度介质在高压灭菌前后的渗透值,pH值变化状况及其时程。分析了不同介质预处理后多核糖体状况、可溶性蛋白凝胶电泳谱和花粉雄核发育状况的变化。结果表明:多核糖体状况的变化与介质在高压灭菌后的pH差异相关;可溶性蛋白电泳谱的变化与介质渗透值关系较密切,且可能与离子胁迫相关;而雄核发育则与pH值、高渗均有关而以pH值关系更为密切。从试验结果看,可认为:1.高渗介质预处理的起始影响,按照介质种类,可能包括渗进胁迫、pH胁迫、和离子胁迫。2.不同影响的作用位置可能各不相同,时程也可能有异。3.在高渗介质预处理试验设计中应注意介质种类和影响特点的选择,注意对照的设置;即使采用过滤灭菌也应事先做好基础测试工作。

 
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