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  pancreatic
     Studies on the expression and mechanisms of p16~(INK4a)gene in human pancreatic adenocarcinoma
     P16~(INK4a)基因在人腺癌中的表达及其作用机制的研究
短句来源
     Studies on eary diagnosis of human pancreatic carcinoma using genetic diagnosis in pancreatic juice and pancreatic duct brushing——detection of mutations of K-ras and DPC4 in Exon8 and Exon11 and Telomerase activity
     液及管刷检液对腺癌早期诊断的研究——k-ras、DPC4基因突变和端粒酶活性的检测
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     Studies on Oncogene and Tumor Suppressor Gene in the Plasma and the Tumor Tissue from Pancreatic Cancer Patients
     腺癌患者肿瘤组织及外周血浆中癌基因和抑癌基因的研究
短句来源
     Study on the Expression of Survivin PTEN and Smad in Pancreatic Carcinoma and Gastric Cancer Clinical Observation of Chemotherapy to Pancreatic Cancer
     Survivin PTEN和Smad在腺癌和胃癌中表达的研究及腺癌化疗的临床研究
短句来源
     The Clinical & Experimental Research on the K-ras Gene Point Mutations and Monoclonal Antibodies of the Gene Expression Products P21 Protein in Pancreatic Adenocarcinoma
     腺癌K-ras基因点突变的临床应用研究及其表达产物P21蛋白的单克隆抗体实验研究
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  pancreas
     Clinical Research of Surgical Management of Adenocarcinoma of Pancreas
     腺癌外科治疗的临床研究
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     Studies on Pathogenic Character of New Type Duck Hepatitis Virus and Apoptosis of Liver and Pancreas in Infected Ducklings
     新型鸭肝炎病毒致病特性及感染鸭肝细胞凋亡的研究
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     Clinical and Experimental Study of Multisection Spiral CT Perfusion for Normal Pancreas and Pancreatic Cancer
     正常腺和腺癌多层螺旋CT灌注成像的临床及实验研究
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     Laboratory Study of Earlier Diagnoses of the Pancreas Acute Rejection after of the Simulataneous Pancreas and Kidney Transplantation in Pig
     猪肾联合移植腺急性排斥反应早期诊断的实验研究
短句来源
     Analysis of Real Time B Scanning Ultrasonotomography of the Gallbladder, Biliary Tract and Pancreas in 50 Normal Persons
     50例正常人胆、B型超声实时显象初步分析
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  simultaneous pancreas
     Clinical study of surviving in simultaneous pancreas kidney transplantation
     肾联合移植长期存活的临床研究(附5例报告)
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     AIM: To discuss the harvesting and trimming of combined pancreas and kidney grafts from cadaveric donors and to examine the clinical outcome of typeⅡ diabetes with renal failure treated with simultaneous pancreas and kidney transplantation.
     目的 :探讨肾联合移植尸体移植物的切取、修剪方法和移植治疗Ⅱ型糖尿病合并肾功能衰竭的临床效果 .
短句来源
     Monitoring of pancreatic rejection after simultaneous pancreas kidney transplantation
     肾Ⅰ期联合移植术后腺排斥反应监测
短句来源
     Establishment of an animal model of simultaneous pancreas and kidney transplantation in pigs
     肾联合移植动物模型的建立和改进
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     Surgical Consideration on Simultaneous Pancreas Kidney Transplantation and Analysis of Postoperative Complications
     肾同期移植术式选择与并发症分析
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  biliopancreatic
     Methods The model of ANP was established in 90 rats by retrograde injection of 5% sodium taurocholate into biliopancreatic duct.
     方法采用5%牛磺胆酸钠逆行胆管注射建立大鼠ANP肝损害模型,90只大鼠,随机分成三组。
短句来源
     Methods The AP model in rats was induced by retrograde injection of 3% sodium taurocholate (STC) into biliopancreatic duct. Ru (15、 30、 60 mg/(kg×h)) was administered by continuous venous infusion (CVI) for 6 hours immediately after the induction of AP.
     方法:3%STC逆行胆管注射诱导大鼠AP模型,造模后立即通过股静脉持续输注(continuous venous infusion,CVI)Ru(15、30、60mg/(kg×h))6h。
短句来源
     in the control group (n = 6), 5% sodium taurocholate (0. 1 ml/100 g) was infused into the common biliopancreatic duct at a rate of 0. 2 ml/min;
     对照组(6只):按 0.1 ml/100g,0.2 ml/min速度用微量推射泵在胆管内注入5%牛磺胆酸;
短句来源
     SAP group: SAP was induced in male SD rats by the retrograde injection of 5% sodium taurocholate (1ml/kg) into the biliopancreatic duct.
     采用4%水合氯醛(10ml/kg)腹腔注射麻醉,逆行胆管注射5%牛磺胆酸钠(1ml/kg)建立SAP模型。
短句来源
     Methods The AP model in rats was induced by retrograde injection of 3% sodium taurocholate into biliopancreatic duct.
     方法3%牛磺胆酸钠(sodium taurocholate,STC)逆行胆管注射诱导大鼠AP模型,取腺做病理学检查。
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  pancreatic
Inhibitive Effect of Prodigiosin on the Proliferation of Human Malignant Pancreatic Cancer Cells
      
Pancreatic cancer is not only common, but also extremely difficult to treat, for which it has been called "the challenge of the twenty-first century".
      
In this study, we find that prodigiosin could effectively inhibit the proliferation of human pancreatic cancer cells H8898 in a dose-and-time-dependent manner, with an IC50 of 75μmol according to the results of MTT and cell proliferation assays.
      
Prodigiosin also could induce apoptosis of pancreatic cancer cells at low concentration and results in the fragmentation pattern of DNA.
      
All these results demonstrate that prodigiosin can obviously inhibit the proliferation of pancreatic cancer cells H8898 by arresting the cell cycle and inducing apoptosis.
      
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  pancreas
Their hepatopancreas were swollen and pale, accompanied by fatty degeneration, fatty necrosis of hepatocytes, and atrophy of the pancreas.
      
The clinicopathologic features of intraductal papillary mucinous neoplasms of the pancreas
      
An analysis of clinico-pathologic features of intraductal papillary mucinous neoplasm of the pancreas
      
One patient's pancreas was unresectable, two patients underwent a total pancreatectomy, 42 patients had a pancreatecoduodenectomy and five patients had distal pancreatectomy.
      
There are four levels of cells in the liver stem cell lineage: hepatocytes, hepatic stem cells/oval cells, bone marrow stem cells and hepato-pancreas stem cells.
      
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  simultaneous pancreas
In the most popular patient group (simultaneous pancreas-kidney transplantation, SPK) the graft-function rate at 1 year now reaches more than 80 %.
      
Results of 33 simultaneous pancreas and kidney transplantations performed at the San Raffaele Hospital, Milan, Italy are presented.
      
Nineteen patients with endstage renal failure due to Type 1 (insulin-dependent) diabetes mellitus received simultaneous pancreas/kidney transplants using bladder drainage technique.
      
Thus, simultaneous pancreas-kidney transplantation can be performed with a high success rate when using the technique described.
      
The risk of technical failure was lowest for primary, bladder-drained, simultaneous pancreas/kidney, >amp;lt; 12 hour UW stored grafts; year and location of transplant were not significant factors.
      
更多          
  biliopancreatic
Largely malabsorptive procedures include biliopancreatic diversion (BPD) and biliopancreatic diversion with duodenal switch (BPD-DS).
      
For the heaviest, biliopancreatic diversion or biliopancreatic diversion with duodenal switch might be considered.
      
Vitamin B12 (B12) absorption was studied in rats following biliopancreatic duct ligation.
      
Biliopancreatic fistula caused by an intraductal papillary-mucinous tumor of the pancreas confirmed by biochemical analysis of m
      
Intraductal papillary-mucinous tumor of the pancreas is occasionally accompanied by biliopancreatic fistula.
      
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  其他


Observations were made to compare the effect of HCl introduced into the small intestine of dogs on pancreatic secretion in the following three conditions: (1) Acute experiments with barbiturates(pentothal sodium or pentobarbital sodium) as an(?)sthesia; (2) Chronic experimental dogs with Thomas pancreatic fistula; (3) Chronic dogs in(2) an(?)sthetized by means of the same barbiturates as in the acute experiments. The results indicated that in the acute experiments, the latent period of pancreatic secretion was...

Observations were made to compare the effect of HCl introduced into the small intestine of dogs on pancreatic secretion in the following three conditions: (1) Acute experiments with barbiturates(pentothal sodium or pentobarbital sodium) as an(?)sthesia; (2) Chronic experimental dogs with Thomas pancreatic fistula; (3) Chronic dogs in(2) an(?)sthetized by means of the same barbiturates as in the acute experiments. The results indicated that in the acute experiments, the latent period of pancreatic secretion was about 3-5 minutes and the amount secreted was usually below 20 drops in the first 30 minutes after the introduction of the acid. But in the case of chronic experiments, the results were quite different. The latent period was shorter, usually less than 2 minutes and the amount of pancreatic secretion was usually 10 times as much as in the acute experiments. Moreover, the pancreatic secretion of the chronic fistula dogs was not changed either in latent period or in amount when under the barbiturate an(?)sthesia. From the above results, it is quite natural to assume that the influence of the barbiturate an(?)sthetics, pentothal sodium and pentobarbital sodium on pancreatic secretion induced by HCl in the intestine of dogs is negligible. The great difference in the pancreatic secretion between the acute and the chronic experiments might, therefore, be due to the operative trauma which is unavoidable in acute experiments. Another observation was made to determine the effect of atropine on pancreatic secretion induced by HCl in chronic fistula dogs. The result showed that atropine distinctly reduced the response of pancreas to the acid. The latent period was increased and the amount of pancreatic secretion was decreased as compared with the result obtained in normal chronic fistula dogs. Hence it is quite suggestive that there might exist a nervous component in the mechanism of pancreatic secretion induced by HCl in the small intestine. We wish to express our gratitude to Comrade for his valuable advice and to professors T. C. Shen and T. F. Liu for their encouragement throughout this work.

本實驗比較急性實驗狗、慢性瘻狗和經過麻醉的慢性屢狗對於鹽酸注入小腸所引起的液分泌量和潛伏期,結果證明: (1)在急性實驗情况下,狗腺對鹽酸刺激小腸所引起的液分泌量遠較在慢性實驗時為少,且潛伏期較長。 (2)巴比妥類麻醉劑:硫賁妥鈉(sodium pentothal)和戊烷巴比妥鈉(sodiumpentobarbital)對鹽酸所引起的液分泌量及潛伏期影響極微。 (3)在急性實驗情况下,由鹽酸所引起的液分泌量的減少和潛伏期的加長,似乎不是由於巴比妥類麻醉劑的作用,而可能是由於手術創傷的影響。 (4)注射阿托平後,腺對於鹽酸刺激小腸所引起的反應顯著减小,故推测在鹽酸引起液分泌的機制中可能有神經反射作用的參與。本工作在进行過程中,承蘇聯專家同志親切地給予指導,并承沈(?)淇、劉曾復二教授关懷和支持,(?)此誌謝。

1. Les cellules pancréatiques exocrines du cobaye, du lapin et du souris blanc ontété étudiées. Nous avons constamment appliqué la méthode convergeante; dans tous lescas, l'étude cytologique d'une espèce a comportè la mise en ceuvre de techniques vitales (rouge neutre, blue crésel brillant et vert Janus), mitochondriales, d'imprégnation métallique et osmique, et histochimiques.2. Notre recherche tend à résoudre les questions suivantes: (a) cn dehors du noyau et du centrosome, quels sont les constituants morphologiques...

1. Les cellules pancréatiques exocrines du cobaye, du lapin et du souris blanc ontété étudiées. Nous avons constamment appliqué la méthode convergeante; dans tous lescas, l'étude cytologique d'une espèce a comportè la mise en ceuvre de techniques vitales (rouge neutre, blue crésel brillant et vert Janus), mitochondriales, d'imprégnation métallique et osmique, et histochimiques.2. Notre recherche tend à résoudre les questions suivantes: (a) cn dehors du noyau et du centrosome, quels sont les constituants morphologiques fundamentaux du cytoplasme de la cellue pancréatique exocrine? (b) La théorie du vacuome de l'Ecole de Parat et the "Golgi artifact theory" de l'Ecole de Baker s'appliquent-elles aussi sur cette cellule? L'appareil de Golgi existe-il dans la cellule pancréatique exocrine vivante? (c) Dans cette cellule, existe t-il une "Zone de Golgi," (Parat) composée du vacuome, du chondriome périvacuolaire et des lipoides diffus? si oui, quels sont les rapports entre cette zone et le soi-disant l'appareil de Golgi? (d) Quelles sont les relations entre le vacuome, le chondriome, l'appzreiI, de Golgi et les grains de secrétion. La théorie de la naissance des grains de secrétion de la cellule pancréatique au dépens du chondriome, défendue brillamment par les uns et attaquée vivement par les autres, est-elle exacte?3. Observations vitales—la cellule pancréatique exocrine du cobaye est caractérisée par la taille très différente des grains de secrétion, selon le degré de maturité auquel ils sont parvenus. Les grains murs sont très petits et sont accumulés è l'apex de la cellule, cn face de la cavite d' excrétion, en attendant d' etre expulsès. Les grains volumineux qui sont moins murs sont concentrés audessus d'une plage immédiatement juxta-nucléaire.Dans la plage en question, il existe un nombre assez considérable de petites vésicules. colorées de facon homogène et intense par le rouge neutre, ou par le blue crésyl brillant. Il s'agit de vacuoles du type plasmocrines de Renaut. Ces vacuoles se sont groupées. plutt au centre de la zone vacuolaire. Autour d'eux, et aussi entre les grains desecrétion et les petites vacuoles, se trouvent des vacuoles plus grosses colorées en rouge rose, rouge orange ou rouge doré, scion l'état de condensation du contenu vacuolaire.Parni ces vacuoles, se trouvent quelques petites vésicules renfermant un grain refringcant. La coloration vitale au rouge neutre met parfaitement en évidence ces vésicules, mais laisse les grains incolores. Il s'agit donc des vacuoles rhagiocrines de Renaut, Les vacuoles rhagiocrines se présentent en coupe optique comme un anneou ou un croissant colorable par le rouge neutre, tandis le grain inclus dans Ia vacuole reste toujours incolore, comme les grains de secrétion eux-memes non plus, ils ne sont jamais colorés par les colorants basiques. Les vacuoles plasmocrines précédent toujòurs Ies rhagiocrines. Les vacuoles plasmocrines sont les plus jeunes; tandis que les vacuoles rhagiocrines sont plus évoluées, ayant condensé leur contenu. Ces vacuoles en voie de condensation, ont tendence à s' écarter vers la région supérieure de l'aire vacuolaire. Rapidement, d'aiIleurs, elles perdent leur colorabilité, par le rouge neutre, la coque colorable diminuant en meme temps que le grain grassit.La coloration au vert Janus est fort belle et instructive: elle nous montre des chondriocontes filamenteux, lisses, allongés dans, l' axe de la cellule; quelques-uns cependant, dans la zone infra-nucléaire, tre couchés dans un sens perpendiculaire. C' est le chondriome ordinaire. De plus, dans la zone supra-nucIéaire se trouvent d' autres chondriocontes un peu plus courts dont l'orientation se dispose tout autrement: ils convergent, en effet, vers la plage vacuolaire, serpentent entre les vacuolcs, tout enéant intimement accolés à elles, sans jamais toutefois se résoudre en grains, en chainettes de grains ou vésicules. C' est le chondriome perivacuolaire. Il y a donc au niveau de la plage vacuolaire une intricat

(1)在脊椎动物脏细胞内,根据活观察,活体染色的结果,知道在细胞质内,除胞核及中心体外,只存在着两种独立的,不同的形态组成物:液泡系和线粒体系,在活细胞内绝对没有任何形态的高尔基体,如同在其他动物细胞内一样。 (2)由中性红或亮焦油蓝染色所显示的液泡系,和由金属浸染法所得到的,独立的圆形颗粒的高尔基体——就是属于“dictyosomes”类型,两者是完全符合一致的。 (3)在脏细胞内,如同在其他任何後生动物细胞,尤其是腺细胞内一样,也存在着一个“高尔基区”,出液泡系,环泡线粒体系及弥散类脂体三者共同组成的,这三种组成物可以单独地或彼此混合地被金属浸染成为各式各样的高尔基体,(圆形的、独立的粒状,细网或粗网状,以及“大斑块”状。) (4)脏细胞分泌粒是由液泡直接产生的,和任何线粒体系,不发生任何直接的关系,分泌粒起源于线粒体系的学说,完全是不正确的。 (5)我的研究结果,再一次地证明:巴哈学派的液泡学说和拔克的“人工产物的高尔基体学说,是完全正确的,这两种学说,对脏细胞也完全适用的。

Purified succinic dehydrogenase is a metallo-flavin-adenine protein containing non-haematin iron.The flavin-adenine prosthetic group is firmly bound to the protein part of the enzyme and cannot be split from the latter by boiling in weak acid medium.By digesting with trypsin and chymotrypsin,however,the prosthetic group can be liberated in combination with a peptide chain.The product has been purified by a procedure which involves cresol extraction, mercuric sulphate precipitation,decomposition of the latter...

Purified succinic dehydrogenase is a metallo-flavin-adenine protein containing non-haematin iron.The flavin-adenine prosthetic group is firmly bound to the protein part of the enzyme and cannot be split from the latter by boiling in weak acid medium.By digesting with trypsin and chymotrypsin,however,the prosthetic group can be liberated in combination with a peptide chain.The product has been purified by a procedure which involves cresol extraction, mercuric sulphate precipitation,decomposition of the latter with hydrogen sulphide,followed by paper electrophoresis and paper chromatography.The purified product has been separated into four flavin-adenine peptides with different amino acid contents.One fraction with comparatively high mobility on paper electrophoresis and containing 12 amino acids(hydrolyzed in 6 N HCl) has an absorption spectrum with maxima at 265,350 and 450 mμ(compared with 260,375 and 450 mμ of FAD),the ratio of E_(260) and E_(450) mμ is equal to 3.87.The other three fractions has similar absorption spectra as that of the first,except for a slight shift of the 265 mμ maximum to 270 mμ.All the four flavin-adenine peptides contain cysteine and show a greenish yellow fluorescence in the u.v.light.The fluorescent intensity of the prosthetic group varies with pH and exhibits a maximum at pH 2.9.All fractions are inactive in the D-amino acid oxidase test and give on analysis 1 mole of adenine and 2.5 moles of phosphorus per mole of flavin.The pentose flavin ratio was much higher than that of FAD. Photolysis of the flavin-adenine peptides in alkaline solution yields a product which is insoluble in chloroform after acidification.Removal of the adenine results in the formation of flavin peptides.These facts indicate that the peptide chain is linked to the isoalloxazine nucleus of the prosthetic group.It is known that the absorption peak at 375 mμ of either FAD or FMN shifts to about 355 mμ at pH 12 due probably to the enolization of the keto group at the 2 or 4 position resulting in a redistribution of double bonds in the isoalloxazine ring system. In contrast our flavin-adenine peptide has the corresponding absorption maximum at 350 mμ which shows little positional shift at pH 12.This seems to suggest that the linking of the peptide chain to the isoalloxazine nucleus affects the enolization of the-NH-CO-,which may probably be the site of the linkage. The iron content increases with the specific activity of the enzyme during purification.Iron in the purified enzyme is present in the reduced state.It is firmly bound to the enzyme and can not be removed by prolonged dialysis against phosphate buffer or tris-hydroxymethyl-amino-methane buffer.Enzymatic activity is lost during prolonged incubation with o-phenanthroline or α,α'- dipyridyl and can not be recovered by incubation with Fe~(2+) or Fe~(3+).These experiments de- monstrate a close relationship between enzyme activity and the iron present in the enzyme molecule. The enzyme activity is lower in borate than in phosphate buffer.When 40 mM ethylenedi- aminetetraacetic acid is added to the borate buffer,the enzyme activity is raised almost to the level of that observed in the same concentration of phosphate buffer.The effect of EDTA and phosphate,when present together,is somewhat higher than of either alone.Alanine has a similar effect'as EDTA.

(一)用结晶蛋白酶及结晶凝乳蛋白酶处理净化的水溶性琥珀酸脱氢酶,经过对甲酚抽提,硫酸汞沉淀,硫化氢分解及纸电泳纸层析等方法净化得到四种带有不同肽链的腺嘌呤异咯嗪核苷酸。从它们的组成成份的分析以及它们的性质的观察,我们认为它们与已知的腺嘌呤异咯嗪二核苷酸略有不同。肽链是连接在异咯嗪上,其连接方式异于一般异咯嗪蛋白。肽链部份的氨基酸组成的分析结果,证明它们都含有半胱氨酸。(二)琥珀酸脱氢酶中的铁处于还原状态。铁与酶朊紧密结合,它与酶活力有密切关系。(三)无机磷可增加琥珀酸脱氢酶的活力,但琥珀酸脱氰酶的活力并不是必需依靠无机磷的存在,乙二胺四乙酸与丙氨酸也有类似无机磷的作用。

 
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