The GPA1 gene was obtained via PCR amplification and was cloned in the two-hybrid DNA binding domain vector pGBKT7 The combinant plasmid was designated as pGBKT7-Ga. β-galactosidase assay indicatedthat Got did not have the property of self-activation. After pGBKT7-Ga was transformed to yeast pJ69-4A, we transformed Arabidopsis vegetative tissue two-hybrid cDNA library plasmids to yeast pJ69-4A containing pGBKT7-Ga.
whereas AFL2 expressed in the sepal,ovary,root,stem,leaf and the terminal buds between June to Oct.These results might suggest that AFL1 and AFL2 had a common origin,and their functions had diverged,so that they appeared to play independent roles in floral and vegetative tissue development.
The RT-PCR analysis of its expression in different tissues revealed that it expressed itself in the vegetative tissues,flower buds and other different organs and its expression model was consistent with the AP2 expression model of Arabidopsis and the PhAP2A expression models of Petunia.
Southern hybridization showed that the ZM401 sequence is present in one copy in the maize genome. Northern blots analysis revealed that the expression of ZM401 is restricted to mature pollen and nearly mature anther, and ZM401 transcripts were not detected in leaves, root and other vegetative tissues.
AP2 and PhAP2A, respectively. This reflects the diversity in the function of AP2 gene family except for acting in the nuclear. RT-PCR analysis showed that MAP2 is expressed in floral buds, all floral organs and vegetative organs, which is similar to the expression pattern of AP2 and PhAP2A.
On the other hand,it proves thatthe middle-layer in a normal plant acts really as the nutrition tissue ofthe anther,The abnormality of the vascular system of the anther and theearly aberration of the living-cell indicate that the Ta 1 gene has manife-station not only in the sex cells but also in the somatic cells of the stamen.
The author consider that it is unsuitablefor the destal cut end of an artery or a vein to be usedas the feeding vessel in microvascular free tissue trans-fer , because lowered blood pressure might result in in-adequate tissue perfusion.
The characteristics of temporal and spatial expression of AP2 gene in floral organs andvegetative tissues on the basis of molecular regulating models of floral development in higher plant weredescribed. AP2 participated in flower development process involving the establishment of floral meristemidentity, the specification of flower organ identity and the regulation of floral organogenesis, the temporaland spatial regulation of homologous and heterotypical flower gene activity.