④ the fluorescence signal scan of the gene chip showed that among the 5504 gene clones of the whole chip,there were 208 gene with high expression before treating with the drug,which accounted for 3.8% of the whole clones.
On the basis of the single-band temperature measurement model established in this paper, we have measured the radial temperature distribution of oxygen and argon mixture arcjet by means of the fluorescence signal at the band (10,2) of O2 Schumann-Runge system.
Both GP+envAm12 murine fibroblasts and K562 leukemic cells transduced with EGFP virus demonstrated a stable green fluorescence signal readily detectable by flow cytometry or fluorescence microscopy in up to 97% and 86% of examined cells, respectively.
Methods: Single ventricular myocytes were enzymatically isolated, loaded with fura 2/AM (0.5 μmol/L) for 30 min in dark at room temperature and field stimulated (0.5 Hz, 5 ms), and both myocyte contraction and intracellular fluorescence intensity were simultaneously assessed by a video based motion edge detection system.
Methods mRNA was extracted and purified from the lung of the mouse with LA795 lung metastasis, hybridized respectively on 4 096-gene chip. cDNA microarray was scanned for the fluorescent signals and analyzing difference expression.
Methods The mRNA were extracted respectively from the brain of 2-day-old F1 generation rats whose F0 male generation were tested with and without nonylphenol,then reversely transcribed to cDNA and labeled with cy5 and cy3 fluorescence. Subsequently,the cDNA probes were hybridized to the Mouse 40S cDNA microarray and the fluorescent signals of cy5 and cy3 were scanned and analyzed.
Objective To study the integration of HBV on hepatocellular chromosome of the patients with chronic hepatitis B.Methods Chromosome fluoresent in situ hybridization(FISH) was done with dig-labeled HBV probe in 16 patients with chronic hepatitis B.Results Green fluorescent signals were seen in 5 patients(31.25%),the integration sites were present in low frequency(1.00%-1.50%) and randomly distributied.
Chromosome in-situ hybridization was done with Dig-labeled HBV probe. Green fluorescent signals were seen in 5 patients specimens(31.25%). The integration sites were present in low frequency(1.00%-1.50%) and randomly distributied.
Results At 37 ℃ the free Ca2+ in osteoclast- like cells could be labelled effectively with 10 μmol/L Fluo- 3/AM, the intensity of Ca2+ fluorescent signal in the central part was greater than that in the peripheral part and in the same section the signal was not distributed evenly.
The slope of the standard curve was-3.32, correlation coefficient was -0.994. The optimum primer and MgCl2 concentration for TGF-β1 and actin were 0.8μmol and 2.5mmol,0.5μmol and 3.5mmol,respectively. Fluorescent signal was collected at 85℃.
The microwave field induced magnetic dipole transitions between the magnetic sublevels of the 5S (F=2) and 5S (F=3) states, resulting in a change in the fluorescence signal.
The second type of energy trap gives the dominant contribution to the fluorescence signal at a registration wavelength having the oblique geometry or orthogonal direction of the transition dipole moments of the interacting pyroPheo molecules.
The resulting fluorescence signal shows a linear response to the quantity of solute present over 2 to 3 orders of magnitude (correlation coefficients: 0.990-0.98).
It was necessary to use acetic acid in the mobile phase to achieve good separation, but this led to fluorescence signal suppression, because puerarin and daidzein have native fluorescence at pH 8.0-9.0.
Results: After gene transfection and ping-pong transduction, amphotropic producer line Am12/LGSN was generated with a stable green fluorescence signal readily detectable by FCM in up to 97% of examined cells.
Changes in fluorescent signals of DSM were found to depend on the EPO concentration and physiological status of thymus cells.
The four specific oligonucleotide probes including the matched and the mismatched by the fluorescent target sequence gave obviously different hybridization fluorescent signals.
The mixed probes of each sample were then hybridized with 4,096 cDNA arrays (4,000 unique human cDNA sequences), and the fluorescent signals were scanned by ScanArray 3000 scanner (General Scanning, Inc.).
Following an enrichment of mutant cells by use of 9-(1'-pyrene)nonanol/ultraviolet irradiation (P9OH/UV) method, five peroxisome-defective mutants were isolated by pursuing the fluorescent signals from GFP.
The slides were studied with a fluorescence microscope and the percentages of positive nuclei with aberrant fluorescent signals were counted.
One of the vibrational levels of CrO_2Cl_2 was excited with R28 branch of TEA CO_2 laser. Fluorescence of electronic state of the molecule is observed. After analyzing multiple photon absorption kinetics process qualitively, with the signal of the fluorescence, we believe that it is possible to transfer vibration energy of the vibration-excited molecule to electronic state.
The infrared absorption spectrum of ethene(C_2H_4) was measured by a new method, laser difference spectrum with resolution much better than that of normal spectrometer. Output from OPO laser was monitored and regulated efficiently by means of this method, so that the time-resolved fluorescence of excited methane(CH_4)which has high S/N ratio was observed.