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荧光信号     
相关语句
  fluorescence signal
     Addition of 50 g/L HCl medium and 20 g/L thiourea-ascorbic acid solution produced strongest and stable fluorescence signal.
     加入50g/L盐酸介质和20g/L硫脲-抗坏血酸溶液,得到的荧光信号强度最大且稳定;
短句来源
     When probe concentration was more than 31 μmol/L, the fluorescence signal reached flat roof location.
     点样的探针浓度达到 31μmol/L以上 ,荧光信号为平台期。
短句来源
     ④ the fluorescence signal scan of the gene chip showed that among the 5504 gene clones of the whole chip,there were 208 gene with high expression before treating with the drug,which accounted for 3.8% of the whole clones.
     ④基因芯片荧光信号扫描结果显示,整张芯片 5504 个基因克隆,其中,用药前高表达的基因208 个,占克隆总数的3.8%;
短句来源
     On the basis of the single-band temperature measurement model established in this paper, we have measured the radial temperature distribution of oxygen and argon mixture arcjet by means of the fluorescence signal at the band (10,2) of O2 Schumann-Runge system.
     在建立O2单带测温模型和软件的基础上,利用Schumann Runge带系(10,2)带的荧光信号、测量了氧氩混合电弧加热射流的径向温度分布。
短句来源
     Both GP+envAm12 murine fibroblasts and K562 leukemic cells transduced with EGFP virus demonstrated a stable green fluorescence signal readily detectable by flow cytometry or fluorescence microscopy in up to 97% and 86% of examined cells, respectively.
     流式细胞术和荧光显微镜检测发现 ,EGFP病毒转录的GP +envAm12细胞和K5 62细胞均可发出稳定的绿色荧光信号 ,阳性率可分别达 97%和 86% ;
短句来源
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  fluorescence intensity
     Antioxidant catalase(0 2 U·L -1 ) attenuated the ET-1-induced incre ase of fluorescence intensity of intracellular DCF-DA and myocyte hypertrophy.
     过氧化氢酶 (catalase ,CAT ,0 2U·L- 1 )抑制ET 1 (1× 1 0 - 8mol·L- 1 )诱导的心肌细胞内DCF DA的荧光信号的增加和心肌细胞肥大。
短句来源
     Results PNS (0.05, 0.10, and 0.15 g/L) significantly decreased the protein content, 3H-Leu incorporation rate, and Ca 2+ fluorescence intensity compared with the model group.
     结果与模型组相比,0.05、0.10、0.15g/LPNS可显著降低细胞内蛋白质的量和3H-亮氨酸掺入量,减弱细胞内Ca2+荧光信号
短句来源
     RESULTS ①Fluorescence intensity of intracel lular DCF-DA and myocyte hypertrophy increased by ET-1 in dose-dependent mann er.
     结果 ①ET 1浓度依赖性地使心肌细胞内DCF DA的荧光信号增加和心肌细胞肥大。
短句来源
     Methods: Single ventricular myocytes were enzymatically isolated, loaded with fura 2/AM (0.5 μmol/L) for 30 min in dark at room temperature and field stimulated (0.5 Hz, 5 ms), and both myocyte contraction and intracellular fluorescence intensity were simultaneously assessed by a video based motion edge detection system.
     方法常规酶解法分离制备钙耐受心肌细胞; 用Ca2+荧光探剂fura2/AM(0.5μmol/L)负载心肌细胞30min(25℃)后,以含氧台氏液灌流并予电场刺激(0.5Hz,5ms),采用动缘探测系统检测心肌细胞收缩/舒张功能及细胞内荧光信号变化。
短句来源
     ②Simvastatin inhibited the increase of fluorescence intensity of intracellular DCF-DA and cardiac myocyte hypertrophy induced by ET-1(10 -8 mol·L -1 )in a dose-dependent manner.
     ②辛伐他汀对ET 1诱导的心肌细胞内DCF DA的荧光信号增强和心肌细胞肥大产生剂量依赖性的抑制作用。
短句来源
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  fluorescent signals
     In order to decrease noise among fluorescent signals,cutoff value was calculated,and results was showed in the form of(Ssignal-Nnoise).
     为便于结果判定,确定截断(cutoff)值,并以Ssignal与Nnoise差值的形式消除荧光信号中的噪声。
短句来源
     Methods mRNA was extracted and purified from the lung of the mouse with LA795 lung metastasis, hybridized respectively on 4 096-gene chip. cDNA microarray was scanned for the fluorescent signals and analyzing difference expression.
     方法 以LA795小鼠肺腺癌肺转移组织 ,制备mRNA探针 ,应用含有 40 96条鼠基因的cDNA表达谱芯片 ,用ScanArray 3 0 0 0扫描仪扫描芯片荧光信号图像 ,分析肿瘤组织和康莱特治疗后肿瘤组织中差异表达基因。
短句来源
     Methods The mRNA were extracted respectively from the brain of 2-day-old F1 generation rats whose F0 male generation were tested with and without nonylphenol,then reversely transcribed to cDNA and labeled with cy5 and cy3 fluorescence. Subsequently,the cDNA probes were hybridized to the Mouse 40S cDNA microarray and the fluorescent signals of cy5 and cy3 were scanned and analyzed.
     方法 NP染毒F0亲代母鼠 ,分别提取NP组和对照组F1子代 2日龄雄性大鼠的脑组织mRNA ,分别用cy5和cy3逆转录标记 ,与Mouse4 0S基因表达谱芯片杂交 ,并对cy3、cy5荧光信号做扫描分析。
短句来源
     Objective To study the integration of HBV on hepatocellular chromosome of the patients with chronic hepatitis B.Methods Chromosome fluoresent in situ hybridization(FISH) was done with dig-labeled HBV probe in 16 patients with chronic hepatitis B.Results Green fluorescent signals were seen in 5 patients(31.25%),the integration sites were present in low frequency(1.00%-1.50%) and randomly distributied.
     方法选择我院慢性乙型肝炎患者16例,采用荧光原位杂交(FISH)技术制备肝细胞染色体标本,以地高辛标记的HBV探针进行原位杂交。 结果16例患者中,5例(31.25%)肝细胞分裂期染色体上可观察到绿色荧光信号,HBV呈低频率整合(1.00%~1.50%),整合位点呈随机分布。
短句来源
     Chromosome in-situ hybridization was done with Dig-labeled HBV probe. Green fluorescent signals were seen in 5 patients specimens(31.25%). The integration sites were present in low frequency(1.00%-1.50%) and randomly distributied.
     16例患者中,有5例患者的肝细胞分裂期染色体上可观察到绿色荧光信号,占31.25%,HBV呈低频率整合(1.00%~1.50%),整合位点呈随机分布。
短句来源
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  fluorescent signal
     Results At 37 ℃ the free Ca2+ in osteoclast- like cells could be labelled effectively with 10 μmol/L Fluo- 3/AM, the intensity of Ca2+ fluorescent signal in the central part was greater than that in the peripheral part and in the same section the signal was not distributed evenly.
     结果在37℃、Fluo-3/AM终浓度为10μmol/L的条件下,破骨细胞可获得良好的标记效果,破骨细胞中部层面内Ca2+荧光信号的强度较高,同一层面内分布是不均匀的。
短句来源
     The slope of the standard curve was-3.32, correlation coefficient was -0.994. The optimum primer and MgCl2 concentration for TGF-β1 and actin were 0.8μmol and 2.5mmol,0.5μmol and 3.5mmol,respectively. Fluorescent signal was collected at 85℃.
     定量分析用标准曲线斜率为-3.32,相关系数为-0.994,误差为0.016。 大鼠TGF-bate1表达水平的SYBRGreen实时荧光定量PCR方法,TGF-bate1和Bate-actin的最佳MgCl2反应浓度均为3.5mmol,引物浓度前者为0.8μmol,后者为0.5μmol,在87℃时收集荧光信号
短句来源
     Advances in the Research of Molecular Fluorescent Signal and Photocontrol Molecular Switching Devices
     分子荧光信号器件和光控分子开关研究的现状和进展
短句来源
     The exact genotyping results of 344 Bloodstains were obtained ,having strong fluorescent signal and low background.
     344份血样均得到明确的分型结果,获得的荧光信号强,本底低。
短句来源
     In order to study the fluorescent signal and intensity of FDS NP in the cultured cells,laser scanning confocal microscope and the analysis of flow cytometry were utilized.
     将荧光纳米颗粒与培养细胞共培育后,通过激光扫描共聚焦显微镜和流式细胞仪研究了不同时间点细胞内荧光信号和荧光强度.
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  fluorescence signal
The microwave field induced magnetic dipole transitions between the magnetic sublevels of the 5S (F=2) and 5S (F=3) states, resulting in a change in the fluorescence signal.
      
The second type of energy trap gives the dominant contribution to the fluorescence signal at a registration wavelength having the oblique geometry or orthogonal direction of the transition dipole moments of the interacting pyroPheo molecules.
      
The resulting fluorescence signal shows a linear response to the quantity of solute present over 2 to 3 orders of magnitude (correlation coefficients: 0.990-0.98).
      
It was necessary to use acetic acid in the mobile phase to achieve good separation, but this led to fluorescence signal suppression, because puerarin and daidzein have native fluorescence at pH 8.0-9.0.
      
Results: After gene transfection and ping-pong transduction, amphotropic producer line Am12/LGSN was generated with a stable green fluorescence signal readily detectable by FCM in up to 97% of examined cells.
      
更多          
  fluorescence intensity
The results indicated that the weak election-withdrawing group 4-hydroxymethyl in 4-position of pyridine in 4-HMDPA could weaken the fluorescence intensity of the lanthanide complexes.
      
The method for calculating the binding-site number in BSA for Indo-1 was developed based on the relationships between changes in Indo-1 fluorescence intensity and the analytical concentration of BSA.
      
Fluorescence intensity studies of Triassic acritarchs from the Yanchang Formation in Ordos basin, northwestern China
      
In accordance with the fluorescence intensity of organic cell walls, two groups of microfossils were distinguished.
      
The positive percentage and the average fluorescence intensity of platelet membrane GP Ib were detected by full-blood flow cytometry.
      
更多          
  fluorescent signals
Changes in fluorescent signals of DSM were found to depend on the EPO concentration and physiological status of thymus cells.
      
The four specific oligonucleotide probes including the matched and the mismatched by the fluorescent target sequence gave obviously different hybridization fluorescent signals.
      
The mixed probes of each sample were then hybridized with 4,096 cDNA arrays (4,000 unique human cDNA sequences), and the fluorescent signals were scanned by ScanArray 3000 scanner (General Scanning, Inc.).
      
Following an enrichment of mutant cells by use of 9-(1'-pyrene)nonanol/ultraviolet irradiation (P9OH/UV) method, five peroxisome-defective mutants were isolated by pursuing the fluorescent signals from GFP.
      
The slides were studied with a fluorescence microscope and the percentages of positive nuclei with aberrant fluorescent signals were counted.
      
更多          
  fluorescent signal
The fluorescent signal results were acquired by scanner ScanArray 4000 and analyzed with software GenePix Pro 3.0.
      
Methods enabling designing of probes with a minimum initial level of the fluorescent signal are examined and analyzed.
      
At later time-points the fluorescent signal appeared granular; at these times the injected material was largely degraded.
      
Different marker selection thresholds for average fluorescent signal intensity and marker frequency were used to create eight extra restricted data subsets.
      
The specifity of the fluorescent signal and the Chromatographic conditions allow to work without any special preparation of the sample.
      
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  其他


One of the vibrational levels of CrO_2Cl_2 was excited with R28 branch of TEA CO_2 laser. Fluorescence of electronic state of the molecule is observed. After analyzing multiple photon absorption kinetics process qualitively, with the signal of the fluorescence, we believe that it is possible to transfer vibration energy of the vibration-excited molecule to electronic state.

用TEA CO_2激光器的R28支线激发铬酰氯(CrO_2Cl_2)分子的某一振动能级,可观察到该分子的电子态荧光。定性地分析了荧光信号的动力学过程,指示经过多次碰撞后,分子较低振动态的能量向上传递到电子态是完全可能的。

Study on the mechanism of Ne 1s2-2p anomalous POGS in HCD was carried out by measuring two-step excited POGS and LIP signals simultaneously. Theoretical analysis is also presented in this paper.

本文报道利用脉冲可调谐染料激光器在Ne HCD灯中测定Ne 1s_2-2p及1s_2-2p一高激发态跃迁的光电流信号的同时利用激光感生荧光技术测量Ne 2p-1s态的感生荧光信号,进一步研究Ne 1s_2-2p态反常脉冲光电流信号的机理。

The infrared absorption spectrum of ethene(C_2H_4) was measured by a new method, laser difference spectrum with resolution much better than that of normal spectrometer. Output from OPO laser was monitored and regulated efficiently by means of this method, so that the time-resolved fluorescence of excited methane(CH_4)which has high S/N ratio was observed.

本文用激光导数光谱技术测量了C_2H_4分子的红外吸收光谱,其分辨率远高于常规的红外光谱仪所得到的结果;利用这一技术司以有效地监视和控制红外调谐的OPO激光输出频率,使之处在CH_4分子的激发振动模以上(~3.3μm),从而获得了信噪比较高的CH_4分子时间分辨的红外荧光信号

 
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