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   荧光信号 在 感染性疾病及传染病 分类中 的翻译结果: 查询用时:0.016秒
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  fluorescent signals
Changes in fluorescent signals of DSM were found to depend on the EPO concentration and physiological status of thymus cells.
      
The four specific oligonucleotide probes including the matched and the mismatched by the fluorescent target sequence gave obviously different hybridization fluorescent signals.
      
The mixed probes of each sample were then hybridized with 4,096 cDNA arrays (4,000 unique human cDNA sequences), and the fluorescent signals were scanned by ScanArray 3000 scanner (General Scanning, Inc.).
      
Following an enrichment of mutant cells by use of 9-(1'-pyrene)nonanol/ultraviolet irradiation (P9OH/UV) method, five peroxisome-defective mutants were isolated by pursuing the fluorescent signals from GFP.
      
The slides were studied with a fluorescence microscope and the percentages of positive nuclei with aberrant fluorescent signals were counted.
      
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Objective Using fluorescence in-situ hybridiztion (FISH) to detect the integration of HBV on PBMC chromosome of pregnant women. Methods 136 HBV carrier mothers and 28 healthy women in our department were selected to set up the PBMC chromosome specimens. Chromosome in-situ hybridization was done with Dig-labeled HBV probe. Results Green fluorescent signals were seen in 4 patient specimens ( 2.94%). All four patients possessed all positive HBsAg and HBeAg mothers. The integration sites were present in low...

Objective Using fluorescence in-situ hybridiztion (FISH) to detect the integration of HBV on PBMC chromosome of pregnant women. Methods 136 HBV carrier mothers and 28 healthy women in our department were selected to set up the PBMC chromosome specimens. Chromosome in-situ hybridization was done with Dig-labeled HBV probe. Results Green fluorescent signals were seen in 4 patient specimens ( 2.94%). All four patients possessed all positive HBsAg and HBeAg mothers. The integration sites were present in low frequency and randomly distributied. There were no positive singals in HBsAg(+)/HBeAg(-) group and control group. Conclusion Using fluorescence in-situ hybridization technique could detect HBV integration on the PBMC chromosome. HBV integrating into PBMC chromosome of pregnant women not only can lead to vertical transmission, but also may result in genetic heredity, which is a high risk factor for hereditary diseases and liver cancer.

目的 采用荧光原位杂交 (FISH)技术检测乙型肝炎病毒 (HBV)携带孕妇外周血淋巴细胞染色体 ,明确是否存在HBV整合。方法 选择我院分娩者中 136例HBV携带孕妇及 2 8名健康对照者 ,制备其外周血淋巴细胞染色体标本 ,以地高辛标记的HBV探针进行原位杂交。结果  136例中 ,有 4例患者的外周血淋巴细胞分裂期染色体上可观察到绿色荧光信号 ,占 2 .94 % ,均为双阳组孕妇 ,HBV呈低频率整合 (1.0 0 %~ 1.6 7% ) ,整合位点呈随机分布。 84例单阳组[HBsAg(+) ]孕妇及 2 8名健康对照标本中均未检出阳性信号。结论 应用FISH技术可检测外周血淋巴细胞染色体上整合型HBV。HBV携带孕妇的外周血淋巴细胞染色体上存在整合型HBV ,使妊娠期HBV感染的危害不仅为母婴间的垂直传播 ,而且可能发生基因遗传 ,成为一种遗传性传染性疾病和肝癌高发的重要危险因素

Objectives: To study molecule biology mechanism and diagnose method in patients with liver cirrhosis by cDNA microarray skill. Methods: Microarrays consisting of 8192 human cDNAs and labeled cDNAs prepared from peripheral blood monocyte(PBMC) in both 15 liver cirrhosis(LC) patients and 15 Chronic hepatitis B(CHB) patients were applied to analyze rene expression. Relative ratios of gene expression in individuals were obtained by comparing the hybridization results, by GenePix 4000B scanning and by ImaGene3.0...

Objectives: To study molecule biology mechanism and diagnose method in patients with liver cirrhosis by cDNA microarray skill. Methods: Microarrays consisting of 8192 human cDNAs and labeled cDNAs prepared from peripheral blood monocyte(PBMC) in both 15 liver cirrhosis(LC) patients and 15 Chronic hepatitis B(CHB) patients were applied to analyze rene expression. Relative ratios of gene expression in individuals were obtained by comparing the hybridization results, by GenePix 4000B scanning and by ImaGene3.0 software analysis. Results: The 60 difference genes(0.73%) filtrated in two groups. Most of the genes(71.6%) involved in inflammatory response and apoptosis,cell proliferation. Conclusion: A lot of biology information about molecule biology mechanism and diagnose method in patients with liver cirrhosis were offered by studies with cDNA microarray skill.

目的 :探索基因表达谱技术在肝硬化形成的分子生物学机制研究及其诊断方法研究的应用。方法 :应用含 81 92个人体cDNA的微阵列芯片和来自外周血单核细胞的标记cDNA ,分析慢性乙型肝炎、肝炎肝硬化各 1 5例基因表达谱。通过GenePix40 0 0B扫描芯片仪和ImaGene3.0软件分析Cy3、Cy5两种荧光信号的强度和比值。结果 :在 81 92个基因中 ,2组中筛选出有差异的基因 60个 ,占 0 .73% ,其中主要是炎症、凋亡基因、细胞外基质蛋白基因、细胞生长调节基因 ,占 71 .6%。结论 :基因表达谱技术可为乙型肝炎肝硬化形成的分子生物学机制及其诊断研究提供大量有益的生物学信息

Objective To explore whether autoimmune phenomena exist in SARS patients, and to seek for unusual autoimmune antibodies in SARS patients. Methods Autoantibodies against cell nuclei (ANA), autoantibodies against smooth muscles (SMA), autoantibodies agaist parietal cells (PCA), autoantibodies agaist heart cells (HRA) were detected by using immunofluorescence, and autoantibodies against live-kidney microsomes (LKM) and anti-M2 antibodies were detected by ELISA in sera taken from 27 SARS patients and 18 healthy...

Objective To explore whether autoimmune phenomena exist in SARS patients, and to seek for unusual autoimmune antibodies in SARS patients. Methods Autoantibodies against cell nuclei (ANA), autoantibodies against smooth muscles (SMA), autoantibodies agaist parietal cells (PCA), autoantibodies agaist heart cells (HRA) were detected by using immunofluorescence, and autoantibodies against live-kidney microsomes (LKM) and anti-M2 antibodies were detected by ELISA in sera taken from 27 SARS patients and 18 healthy controls. Immunofluorescence was used to localize the targets antigens in slides with biochips of lung (monkey) of SARS associated antibodies. Results ANA, AMA, LKM and SMA were found positive in 3,1,1, and 1 of the 27 SARS sera. In 18 healthy control sera, one ANA and one AMA were positive. Statistical analysis showed that there were no difference between two groups in every item detected. Twenty-six of 27 SARS patients and 5 of 18 healthy controls had strongly stained columnar epithelia of the bronchiole, especially the lumen border of the epithelia, the difference between two groups was significant. Conclusion No antibodies against organs but lung were found in SARS patients. There are auto antibodies against lung tissues in sera of SARS patients.

目的 探讨严重急性呼吸综合征(SARS)患者是否存在着自身免疫现象,寻找患者体内异常出现的抗自身组织器官的抗体。方法 随机选取2 7例SARS患者及18例健康献血员对照血清同时应用免疫荧光法检测抗核抗体(ANA)、抗平滑肌抗体(SMA)、抗线粒体抗体(AMA)、抗胃壁细胞抗体(PCA)、抗心肌抗体(HRA) ,应用酶联免疫吸附试验(ELISA)检测抗肝肾微粒体抗体(LKM)及抗线粒体M2亚型抗体,同时利用猴肺组织基质片应用免疫荧光法定位血清中的SARS相关抗体的靶细胞。结果 2 7例SARS患者中有3例ANA阳性、1例SMA阳性、1例AMA阳性、1例LKM阳性、其余均为阴性;18例献血员中有1例ANA阳性、1例AMA阳性,其余均为阴性。统计分析表明SARS患者与献血员间各项自身抗体阳性率差异均不具有统计学意义。在2 7例SARS患者血清中有2 6例在肺组织细小支气管柱状上皮细胞的腔面尖端呈现强阳性荧光信号,献血员中有5例,统计分析表明两者阳性率差异有统计学意义。结论 SARS患者未出现抗肺外组织、器官的自身抗体。患者血清中出现了抗肺组织的自身抗体,定位于细小支气管柱状上皮细胞。

 
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