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  fluorescence intensity
The results indicated that the weak election-withdrawing group 4-hydroxymethyl in 4-position of pyridine in 4-HMDPA could weaken the fluorescence intensity of the lanthanide complexes.
      
The method for calculating the binding-site number in BSA for Indo-1 was developed based on the relationships between changes in Indo-1 fluorescence intensity and the analytical concentration of BSA.
      
Fluorescence intensity studies of Triassic acritarchs from the Yanchang Formation in Ordos basin, northwestern China
      
In accordance with the fluorescence intensity of organic cell walls, two groups of microfossils were distinguished.
      
The positive percentage and the average fluorescence intensity of platelet membrane GP Ib were detected by full-blood flow cytometry.
      
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AIM:To develop an HPLC assay for simultaneous determination of AD202 and its metabolites and to examine metabolites of AD202 in urine of rats. METHODS: Reverse-phase HPLC with fluorescence detection and gradient elutionusing a Waters Chromato-graph equipped with a 710 B WIFP autosam-pler,a power Mate SX plus computer,C18 Nova-pak?4um) 5 mm X10 cm radial compression column connected with a guard micro-column, and a Waters 991 photodiodearray detector for online observation of UV spectrum of related fraction....

AIM:To develop an HPLC assay for simultaneous determination of AD202 and its metabolites and to examine metabolites of AD202 in urine of rats. METHODS: Reverse-phase HPLC with fluorescence detection and gradient elutionusing a Waters Chromato-graph equipped with a 710 B WIFP autosam-pler,a power Mate SX plus computer,C18 Nova-pak?4um) 5 mm X10 cm radial compression column connected with a guard micro-column, and a Waters 991 photodiodearray detector for online observation of UV spectrum of related fraction. RESULTS: Detection limit was 1 -3 ng for AD202 and 1 - 3 ng for its metabolites per injection. After iv AD202 20mg.kg-1, only 4. 9 % dose as total anthracycline fluorescence signal was recovered in urine over 72 h. The predominant urinary metabolites were AD285 (desacyl AD202 ) and AD284(N-mono-debutyl AD285). Six minor metabolites including aglycones and 13-keto reductive product were identified and 3 as-yet unknown metabolites were seen. Enzymatic and acid-hydrolysis failed to reveal the presence of glucuronides in urine. CONCLUSION: The sample analysis technigues developed in this study proved to be very efficient, sensitive, and specific, a total of 11 compounds achieved resolution with detection limit of 2 ng and no interference from matrix. Urine sample can be injected directly into chromatograph without any extraction , making sample analysis simple and time-saving.

目的,同时测定大鼠尿中N,N-二(正丁基)阿霉素-14-戊酸酯及其8种代谢产物 方法:建立了一种反相高压液相色谱法,大鼠iv 20mg·kg~(-1)原药后,其尿直接进样.梯度洗脱,荧光检测.结果:原药最低检出量2 ng,代谢物1—3 ng.被检物不受尿成分干扰.72 h尿中总葸环荧光信号仅为剂量的4.9%,其中主要为脱酰基以及N-脱丁基代谢物.6种次要代谢物包括苷元以及13—酮基还原性代谢物等,但未检出葡萄糖醛酸结合物.结论:本法简便易行,灵敏度高,特异性强。

DNA chip is a new molecular biology technology rapidly developed in recent years. It fixes thousands of oligonucleotide on a silicon chip of 1 cm 2, hybridizes the measuring materials marked with fluorescein or isotope with probe in DNA chip,and gets the fluorescent signal of hybridized probe through the scan of confocalmicroscope. This technology has been extensively used in HGP, DNA sequencing, transcript analysis, gene diagnosis etc. Toxiology is an important branch of life science. DNA chip can be extensively...

DNA chip is a new molecular biology technology rapidly developed in recent years. It fixes thousands of oligonucleotide on a silicon chip of 1 cm 2, hybridizes the measuring materials marked with fluorescein or isotope with probe in DNA chip,and gets the fluorescent signal of hybridized probe through the scan of confocalmicroscope. This technology has been extensively used in HGP, DNA sequencing, transcript analysis, gene diagnosis etc. Toxiology is an important branch of life science. DNA chip can be extensively used in the toxiological field. This paper reviewed the DNA chip technology in mechanism, manufacturing method, signal check, advantages and application prospect in toxiology.

DNA芯片技术是近年来迅速发展起来的分子生物学技术 ,它将成千上万个寡核苷酸固定在厘米大小的硅片上 ,将待测的材料用荧光素或同位素标记 ,在DNA芯片上与探针杂交 ,通过激光共聚焦显微镜对芯片进行扫描获取杂交探针的荧光信号。该技术在人类基因组计划、DNA测序、转录情况分析、基因诊断等方面得到广泛应用。毒理学是生命科学的重要分支之一 ,DNA芯片技术在毒理学中也有潜在的广泛应用前景。本文就DNA芯片技术的原理 ,制作方法 ,信号检测 ,优点及在毒理学中的应用前景进行综述

Objective: To evaluate the feasibility and accuracy of continuous monitoring of drug with fiber optic fluorometry in animal model. Methods: An accurate optical design was used to enhance the intensity of light from a 100 - micron optic fiber and the fluorescence signal can be detected. Daunorubicin (DNR) is determined by fiber optic fluorometry system based on the feature fluorescence spectrum. In a simple animal model, the carotid artery was catheterized with a cannula, housing a 100 - micron optic fiber. The...

Objective: To evaluate the feasibility and accuracy of continuous monitoring of drug with fiber optic fluorometry in animal model. Methods: An accurate optical design was used to enhance the intensity of light from a 100 - micron optic fiber and the fluorescence signal can be detected. Daunorubicin (DNR) is determined by fiber optic fluorometry system based on the feature fluorescence spectrum. In a simple animal model, the carotid artery was catheterized with a cannula, housing a 100 - micron optic fiber. The vary quenching indicated the concentration of DNR in rabbit's blood. Results: The average recovery of all the tested compounds within the set concentration range was 99. 9% - 108. 0% , the within - day reproducibility value were acceptable within 2. 5% -5. 3% respectively. The method permitted detection limits as low as 0. 05 μg·mL-1 at a signal - to - noise ratio of 3. Conclusion: The method is potentially useful for monitoring drug blood concentration in biomedical field.

目的:探索将光纤传感器作为一种新的获取分析物信息的方法用于体内药物过程监测的可行性。方法:通过光的准确聚焦,使进入直径为100μm的光纤中的光强度增加,能够进行荧光信号的检测;同时设计了简便可行的动物模型,使得光纤探头可以直接插入血管对检测对象进行检测分析;以柔红霉素的特征荧光作为传感器对药物的识别分离手段,进行了柔红霉素在家兔体内过程的监测分析。结果:方法的回收率在规定的浓度下为99.9%~108.0%,日内RSD为2.5%~5.3%,当信噪比为3时,检出限为0.05μg·mL~(-1)。结论:此法简便,为在线在位监测其他生物体液中药物浓度及药物在生物体组织器官中的分布代谢提供了可以借鉴的新方法和新技术。

 
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