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   移行上皮细胞 在 生物学 分类中 的翻译结果: 查询用时:0.139秒
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移行上皮细胞
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  transitional epithelial cells
CK 5/6 and CK 14 also stained the cytoplasm of basal cells of esophageal stratified squamous epithelium and transitional epithelial cells of the bladder.
      
Earlier statements to the contrary, the present study demonstrates the presence of a cell surface coat (glycocalyx) on the luminal plasma membrane of the super ficial transitional epithelial cells lining the urinary bladder of male Buffalo rats.
      
Glomeruli were unreactive, and transitional epithelial cells of the ureter and unnary bladder had no detectable Pgp.
      
The growth of transitional epithelial cells with different growth media and growth supports was examined.
      
We made no attempts to distinguish squamous from transitional epithelial cells in our adherence tests.
      
  transitional epithelium
The transitional epithelium of the renal pelvis and the urinary bladder showed hyperplasia, papillomas or carcinomas.
      
In addition, hyperplasia of the transitional epithelium of the renal pelvis was found while the renal cortex and the urinary bladder remained normal.
      
The Lyodura and peritoneum grafts were both progressively resorbed, and replaced by fibroblasts which formed a mature scar lined by transitional epithelium, but without smooth muscle regeneration.
      
Marked glycosylation of sublines of both bladder cancer cell lines was found compared with normal human bladder transitional epithelium (assessed cytochemically).
      
This is later covered by creeping of transitional epithelium lining the ureter, forming what is in effect a ureteral nipple.
      
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Objective:To research the etiology of bladder cancer through a simply fast special sensitive method. Methods:Detecting human papilloma virus in 20 cases of carcinoma of bladder and 5 cases of normal bladder mucous membrane with polymerase chain reaction. Results: The detection rate was 40%.The later of the pathologic grade, the lower was the positive rate.(Grade Ⅰ 50%,grade Ⅱ 43%,grade Ⅲ 20%, P >0 05). There was significant correlation between positive results for HPV and histologic grades of the carcinoma....

Objective:To research the etiology of bladder cancer through a simply fast special sensitive method. Methods:Detecting human papilloma virus in 20 cases of carcinoma of bladder and 5 cases of normal bladder mucous membrane with polymerase chain reaction. Results: The detection rate was 40%.The later of the pathologic grade, the lower was the positive rate.(Grade Ⅰ 50%,grade Ⅱ 43%,grade Ⅲ 20%, P >0 05). There was significant correlation between positive results for HPV and histologic grades of the carcinoma. Conclusion: This method was a simply fast special sensitive and had some reference value in the cause of bladder cancer.

目的:为研究简便、快速、特异、灵敏的检测膀胱癌组织中人乳头状瘤病毒(humanpapillomavirus,HPV),借以探讨膀胱癌的病因。方法:采用聚合酶链反应技术行人乳头状瘤病毒DNA检测。结果:正常膀胱粘膜组织中未检出HPV;20例新鲜膀胱移行细胞癌组织中有8例阳性表达,总阳性率为40%,按WHO分级标准,膀胱移行上皮细胞癌Ⅰ级检出率50%,Ⅱ级43%,Ⅲ级20%。结论:正常膀胱粘膜到病变组织HPVDNA从无到有,说明HPV感染与癌的发生有关,并且HPV在膀胱癌中的存在随病理分级的上升其阳性率下降,说明该方法简便、快速、特异、灵敏,对于膀胱癌病因学有一定的参考价值。

Objective To establish a relative simple and convenient serumfree culture

目的建立一种比较简便的人正常尿路上皮细胞无血清培养方法。方法标本组织块用冷消化法分离出尿路上皮细胞,培养于无血清生长培养基。观察传代细胞形态及生长情况,并用TGFβ1抑制细胞增殖试验检验细胞在有血清或无血清培养基中对TGFβ1的反应。结果培养的总成功率为73%,细胞具有典型的移行上皮细胞形态学特征,细胞能在24小时后增殖进入对数生长期,传代9~11次后开始衰退。另外,无血清培养体系比含血清培养体系更能反映出TGFβ1对细胞抑制作用。结论此培养体系具有简单、短期内可扩增出大量纯净上皮细胞、应用范围广和培养成功率高的特点。

Objective To experiment the possibility of allodecellular extracellular matrix for the replacement of ureter. Methods Extracellular matrix was obtained from the ureters of 20 rabbits by decellularization process.60 experimental rabbits were used and a 4cm segment of left ureter was resected in each.In the experimental group of 20 rabbits,the resected ureter was replaced by the allo ureter ECM.In the first control group of 20 rabbits the resected ureter was replaced by allograft transplantation of untreated...

Objective To experiment the possibility of allodecellular extracellular matrix for the replacement of ureter. Methods Extracellular matrix was obtained from the ureters of 20 rabbits by decellularization process.60 experimental rabbits were used and a 4cm segment of left ureter was resected in each.In the experimental group of 20 rabbits,the resected ureter was replaced by the allo ureter ECM.In the first control group of 20 rabbits the resected ureter was replaced by allograft transplantation of untreated ureter.In the second control group of rabbits the resected ureter site was left untreated for autoregeneration.The neoureter segment was studied with electron microscopy and immunohistochemical procedures. Results In the experimental animals,transitional epithelial cells and monocytes could be seen in the ECM area and fully filled up the area after 3 weeks.No stenosis and hydronephrosis were observed on IVU 24 weeks after replacement of the resected ureter.The histological structure was not much different from a normal ureter.In the first control group with allograft transplantation,rejection and necrosis occurred 7~10 days after the transplantation.In the second conrol group of autoregeneration the studied area was embeded with fibrosis tissue.On the basis of the experiment,ureter ECM has been successfully used in clinical practice. Conclusions Alloureter ECM might be an ideal replacement material for ureter.

目的 寻求理想的输尿管替代材料。 方法 将20 只兔双侧输尿管脱细胞处理后制成输尿管细胞外基质(ECM) ;另60 只兔分为3 组:输尿管ECM 移植组( 实验组) ,对照组1 及对照组2 。 结果 实验组术后组织学检查显示1 周时ECM 边缘部腔内面已见移行上皮细胞,管壁有肌细胞长入。6 周时取材ECM 区已修复近正常输尿管组织结构。12 、24 周时IVU 检查;输尿管显影良好,通畅。对照组1 术后7 ~10 天,移植的异体输尿管均坏死。对照组2 输尿管缺损区6 周时均被纤维包裹而明显增粗。在实验取得满意结果的基础上成功应用于临床。 结论 异体脱细胞输尿管细胞外基质为理想的输尿管替代材料。

 
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