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移行上皮细胞
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  transitional epithelial cells
CK 5/6 and CK 14 also stained the cytoplasm of basal cells of esophageal stratified squamous epithelium and transitional epithelial cells of the bladder.
      
Earlier statements to the contrary, the present study demonstrates the presence of a cell surface coat (glycocalyx) on the luminal plasma membrane of the super ficial transitional epithelial cells lining the urinary bladder of male Buffalo rats.
      
Glomeruli were unreactive, and transitional epithelial cells of the ureter and unnary bladder had no detectable Pgp.
      
The growth of transitional epithelial cells with different growth media and growth supports was examined.
      
We made no attempts to distinguish squamous from transitional epithelial cells in our adherence tests.
      


Objective:To research the etiology of bladder cancer through a simply fast special sensitive method. Methods:Detecting human papilloma virus in 20 cases of carcinoma of bladder and 5 cases of normal bladder mucous membrane with polymerase chain reaction. Results: The detection rate was 40%.The later of the pathologic grade, the lower was the positive rate.(Grade Ⅰ 50%,grade Ⅱ 43%,grade Ⅲ 20%, P >0 05). There was significant correlation between positive results for HPV and histologic grades of the carcinoma....

Objective:To research the etiology of bladder cancer through a simply fast special sensitive method. Methods:Detecting human papilloma virus in 20 cases of carcinoma of bladder and 5 cases of normal bladder mucous membrane with polymerase chain reaction. Results: The detection rate was 40%.The later of the pathologic grade, the lower was the positive rate.(Grade Ⅰ 50%,grade Ⅱ 43%,grade Ⅲ 20%, P >0 05). There was significant correlation between positive results for HPV and histologic grades of the carcinoma. Conclusion: This method was a simply fast special sensitive and had some reference value in the cause of bladder cancer.

目的:为研究简便、快速、特异、灵敏的检测膀胱癌组织中人乳头状瘤病毒(humanpapillomavirus,HPV),借以探讨膀胱癌的病因。方法:采用聚合酶链反应技术行人乳头状瘤病毒DNA检测。结果:正常膀胱粘膜组织中未检出HPV;20例新鲜膀胱移行细胞癌组织中有8例阳性表达,总阳性率为40%,按WHO分级标准,膀胱移行上皮细胞癌Ⅰ级检出率50%,Ⅱ级43%,Ⅲ级20%。结论:正常膀胱粘膜到病变组织HPVDNA从无到有,说明HPV感染与癌的发生有关,并且HPV在膀胱癌中的存在随病理分级的上升其阳性率下降,说明该方法简便、快速、特异、灵敏,对于膀胱癌病因学有一定的参考价值。

Objective To establish a relative simple and convenient serumfree culture

目的建立一种比较简便的人正常尿路上皮细胞无血清培养方法。方法标本组织块用冷消化法分离出尿路上皮细胞,培养于无血清生长培养基。观察传代细胞形态及生长情况,并用TGFβ1抑制细胞增殖试验检验细胞在有血清或无血清培养基中对TGFβ1的反应。结果培养的总成功率为73%,细胞具有典型的移行上皮细胞形态学特征,细胞能在24小时后增殖进入对数生长期,传代9~11次后开始衰退。另外,无血清培养体系比含血清培养体系更能反映出TGFβ1对细胞抑制作用。结论此培养体系具有简单、短期内可扩增出大量纯净上皮细胞、应用范围广和培养成功率高的特点。

Objective To establish an efficient and convenient method of rabbit urothelial cell in vitro. Methods Separated urothelial cell were cultured in DMEM/F12(1:1)supplemented with nutrition , such as 10% fetal bovine serum and EGF, et al. Cells were incubated at 37℃ in a humidified atmosphere of 5%CO 2 . And H&E staining , immunocytochemical staining of cytokeratin and transmission electron microscopy were used to verify the cell's origin. Results After cultured 10~14 days, confluent growth was noted. It is...

Objective To establish an efficient and convenient method of rabbit urothelial cell in vitro. Methods Separated urothelial cell were cultured in DMEM/F12(1:1)supplemented with nutrition , such as 10% fetal bovine serum and EGF, et al. Cells were incubated at 37℃ in a humidified atmosphere of 5%CO 2 . And H&E staining , immunocytochemical staining of cytokeratin and transmission electron microscopy were used to verify the cell's origin. Results After cultured 10~14 days, confluent growth was noted. It is verified by methods of cell morphology and immunocytochemistry that the cells were epithelial in origin and not fibroblasts. Conclusion This culture system can amplify a lot of pure urothelial cells in short time with more applicability and high successful culture rate.

目的 建立一种有效、简便的兔膀胱上皮细胞体外原代培养方法。方法 以DMEM F12 (1∶1)为基础培养基 ,添加10 %FCS、EGF等营养成分 ,在 37℃、5 %CO2 的孵箱内培养 ,并行细胞形态学、免疫组化检测。结果 细胞在 10~ 14d融合形成单层 ,细胞形态呈多角形 ,经HE染色、免疫组化、透射电镜证实为移行上皮细胞。结论 此培养方法能在短时间内大量获得移行上皮细胞 ,具有细胞纯度高、成功率高、适用范围广的特点。

 
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