1. As the concentration of intercalation dye SYBR Green Ⅰwasincreased, the melting curve shifted to higher temperature. Rapid temperature transition also shifted melting curve to higher Tm. The melting curve of PCR amplification product was easily identified as low Tm peak and high Tm peak.
1. 随着DNA结合染料SYBR Green Ⅰ浓度升高和温度转变速度(temperature transition rate)的增快，实时荧光定量RT-PCR解链曲线(melting curve)向更高的Tm值迁移；
In order to compare the association of the MTHFR C677T polymorphism with susceptibility to esophageal squamous cell carcinoma (ESCC) in a Northern Chinese population, high-speed real-time PCR and melting curve analysis were used. The MTHFR C677T genotypes were determined in 189 patients with ESCC and 141 unrelated healthy controls.
Non-specific amplification product tended to be at much lower temperature than specific amplification product. The Tms of GAPDH and hTERT non-specific amplification products were 72.8℃ and 71.6℃, respectively. The Tms of GAPDH and hTERT specific amplification products were 87.2℃ and 90.5℃, respectively.
As the temperature increases, a sharp electrochemical signal change in the melting curve of wild-type dsDNA appears.
Phase Diagram and Melting Curve of Iron Obtained Using the Data of Static and Shock-Wave Measurements
Interest shown by geophysicists in the Earth's core has stimulated numerous investigations of the phase diagram of iron and its melting curve in the pressure range up to several megabar and temperature range up to several thousand degrees.
A new procedure is suggested for macroion charge renormalization, which describes the melting curve of charged colloidal liquids as a function of concentration of added salt.
Good agreement is found with the experimentally obtained melting curve without any additional corrections or fitting parameters, in particular, those associated with the inclusion of surface chemical processes.
OBJECTIVE To analyze SNP by oligo DNA microarray.METHODS Melting curves were determined in the DNA microarray,in terms of the thermodynamic traits of DNA duplexes,and the Tm values was calculated for SNP analysis.RESULTS With the special DNA microarray,thermodynamic analysis has done to get the melting curve of the DNA duplexes as well as their Tm values for SNP analysis.CONCLUSION It is likely to do high-throughput SNP analysis by DNA microarray.