Methods MSCs isolated from pig bone barrow were cultured and induced into differentiate into osteocytes. Before induction and on day 1,7,14 and 21 after induction,MHC-Ⅱ was detected with flow cytometer(FCM),stimulation index was examined by One-way MLC,and IL-2 and IL-10 levels in supernatant was determined.
Synthesis of the main contractile proteins, primarily the components of myosin molecule-heavy chain (MHC) and individual isoforms of light chains (MLC1, MLC2, and MLC3)-are encoded by different genes during different ontogenetic stages.
In addition, considerable differences were revealed in the structure of MLC isoforms at different ontogenetic stages.
The definitive fast muscle contained three light chain types, MLC1, MLC2, and MLC3; slow muscle, MLC1 and MLC3; while the larval muscle fibers included a specific larval MLCL in addition to MLC3.
mRNAs of all three types of myosin light chain proteins are expressed in skeletal muscles of both larval and adult stages of loach Misgurnus fossilis (Cobitidae) and these proteins are encoded by different genes (mlc1, mlc2, and mlc3).
No difference was revealed between transcripts from larval stage and adult fish for all three MLC proteins.