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包皮的
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  preputial
     YAG laser application in preputial resection
     YAG激光切除包皮的探讨
短句来源
     Methods Single-cell suspension was obtained by treating the isolated hair follicles and preputial epidermis with pancreatin,then FKCs and EMCs were respectively purified at the second passage. The third passage of FKCs was seeded in 6-well dishes and cultured for 48 hours,then EMCs were seeded into the same dishes in three different ratios (i.e., melanocyte:keratinocyte ratios of 1:2,1:10 and 1:20).
     方法胰酶消化游离毛囊和包皮的表皮,分别获取纯化的角质形成细胞和黑素细胞,第3代毛囊角质形成细胞接种于6孔板中,48h后以表皮黑素细胞:毛囊角质形成细胞为1∶2、1∶10和1∶20的比例接种黑素细胞。
短句来源
  “包皮的”译为未确定词的双语例句
     The Experience in the Circumcision Operation of 600 Cases with the CO_2 Laser Scapel
     600例CO_2激光切除包皮的体会
短句来源
     Results:Survivin was expressed in 52(86.67%)CA cases and 2(20%)prepuce tissues. The expression of survivin was significantly higher in CA than that in prepuce tissues(P<0.01).
     结果:CA组织中survivin的表达率为86.67%,显著高于正常包皮的表达率20%(P<0.01)。
短句来源
     Methods Cultured normal human epidermal keratinocytes isolated from the foreskins of healthy children were irradiated with UVB in a dose of 20, 60 and 120 mJ/cm2, respectively. The mRNA and protein expression of p53 and its downstream molecules p21, mouse double minute 2 (MDM2), Bax, and growth arrest and DNA damage induced gene 45 (GADD45) was detected by reverse transcription -PCR and Western blotting at 0, 2, 24, and 48 h after the irradiation.
     方法以20、60和120mJ/cm2UVB辐射培养的取自健康儿童包皮的正常人表皮角质形成细胞,应用RT-PCR和免疫印迹方法,分别从mRNA水平和蛋白质水平,检测分析UVB辐射后2h、24h和48h,p53及其下游分子MDM2、p21、Bax和GADD45的表达。
短句来源
     This paper presents the preparation of fluoride glass fiber with low optical loss,
     介绍低损耗氟化物玻璃光纤的制备方法,包括高纯原料和光纤的制备,获得氟塑料包皮的氟锆酸盐玻璃光纤在2.4μm处的最低损耗为75dB/km.
短句来源
     Methods Epithelia and fibroblast asts from human foreskin were primarily cultured. Fibroblasts (1×10 5/cm 2) were inoculated on collagen sponge after digestion and cultured for 3 days prior to inoculation of epithelial cells (2×10 5/cm 2) to construct composite skin grafting substitute,and it was then grafted onto full skin loss (FSL) wounds of nude mice. Collagen sponge membrane lacking cell inoculation was used as control.
     方法 来源于人包皮的表皮细胞和成纤维细胞分别原代培养 ,成纤维细胞经消化后接种在胶原海绵膜上 (1× 10 5/cm2 ) ,培养 3d后接种表皮细胞 (2× 10 5/cm2 ) ,构建复合皮肤替代物 ,移植到裸鼠全层皮肤缺损处 ,以无细胞接种的胶原海绵膜作为对照。
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  相似匹配句对
     The study of foreskin epithelium culture in vitro
     兔包皮上皮细胞分离培养和鉴定
短句来源
     YAG laser application in preputial resection
     YAG激光切除包皮探讨
短句来源
     Pseudo ainhum of the penis: a case report
     包皮假性阿洪病1例
短句来源
     the amount of the ripe cells in prepuce gland increased.
     包皮腺成熟细胞数量增多。
短句来源
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  preputial
This work reports on the use of an autologous graft prepared from a patient's preputial cells cultivated on biodegradable polymeric membrane.
      
Both absolute and relative weights of dorsolateral prostate and preputial glands were reduced in a dose-related fashion.
      
There were no treatment-related effects of butyl paraben on the liver, ventral prostates, seminal vesicles, and preputial glands (both in terms of absolute weight and relative to body weight) in any of the study groups.
      
BM and TM decreased epididymal, seminal vesicular, prostate and preputial weights, and injured seminiferous tubules in mice in a dose-dependent fashion.
      
Male Sprague-Dawley rats were castrated at postnatal day 42, and only the rats that showed preputial separation were used in this study.
      
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Antibodies against purified tu-bulin from rabbit brain have been raised in rabbits.The antiserum de-corated microtubules at a dilution of 1: 32 as judged by indirect immuno-fluorescence microscopy. The antiserum was used in in-direct immunofluorescence micros-copy on cultured fibroblasts from fore-skin of Chinese child and specific staining patterns of intracellular microtubules were shown.In interphase cells,an elaborate complex of fine fluorescent microtubule fibers throughout the cytoplasm was obser-ved.The...

Antibodies against purified tu-bulin from rabbit brain have been raised in rabbits.The antiserum de-corated microtubules at a dilution of 1: 32 as judged by indirect immuno-fluorescence microscopy. The antiserum was used in in-direct immunofluorescence micros-copy on cultured fibroblasts from fore-skin of Chinese child and specific staining patterns of intracellular microtubules were shown.In interphase cells,an elaborate complex of fine fluorescent microtubule fibers throughout the cytoplasm was obser-ved.The cytoplasmic microtubule fibers associate with one or two specific fluorescent foci (i.e.Microtubule Or-ganizing Centers,MTOC) in the cy-toplasm near the nucleus and radiate out toward the cell periphery where they either terminate at the plasma membrane or bend and extend alongthe cell surface.This array of fluore-scent microtubule fibers known as cytoplasmic microtubule complex (CMTC) has been described in a wide variety of cells in vitro by other investigators using antitubulin im-munofluorescence.In mitotic cells,the CMTC disappears and is repla-ced by a bright fluorescent mitotic spindle that functions to segregate the chromosomes during mitosis.The en-trance of cells into mitosis and the disassembly of the CMTC is accom-panied by the major alterations in cell shapes from a flattened state to a more rounded appearance.The CMTC begins to re-appear in late telophase-early G_1 phase in daughter cells which are still connected by midbody.The fluorescence is con-centrated within the midbody which is known to contain numerous micro-tubules and probably represents the ??rudiments of the interpolar spindle fibers.After mitosis,both the CMTG and cell shape return to the inter-phase state. The sensitivity of cytoplasmic mi-crotubules to colcemid and cold tem-perature was demonstrated.Cells trea-ted with colcemid (0.06 μg/ml) for 2 hr show disassembly of CMTC and alteration in cell shape from flatten-ed state to a more rounded and irre-gular appearance.When cells are re-moved from colcemid and placed into fresh medium,both the CMTC and cell shape recover to the original state completely in 90 min.Cellsthat have been chilled to 0-4℃ for 1 hr devoid of a CMTC.Warming the cells to 37℃ initiates re-assembly of the CMTC in a manner similar to that observed for colcemid reversal. Control stain with pre-immune serum from the same rabbit showed negative result. Advantages in specific staining of intracellular microtubules by using a lipid-dissovable organic solvent mix-ture (chloroform plus methanol) with omission of formaldehyde in fixation and using non-ionic detergent in stain-ing and washing steps are reported.

本工作用我们自制的兔抗管蛋白血清经过间接免疫荧光染色检查,效价达到1:32,显示出我国小儿包皮成纤维细胞(正常二倍体细胞)内微管的特异分布。我们观察到在间期细胞内的微管(CMTC)系由核附近的微管组织中心(MTOC)发出到达细胞的边沿,或终止于质膜下方,或弯曲沿细胞表面平行分布;胞质内的微管纤维有的紧绕核周,有的伸入细胞突起内与主轴方向平行排列。当细胞进入分裂期(M期)时,不仅CMTC解聚,同时有丝分裂器纺锤体微管出现,细胞从扁平形状变成圆的外形。当有丝分裂完成后,纺锤体微管荧光消失,CMTC又逐渐代替而出现,细胞也恢复成为扁平形。CMTC在早G_1期开始出现,此时的子细胞之间由于极间微管束的残迹而呈现出荧光染色的阳性反应,是为中体。用秋水仙胺(0.06微克/毫升)在37℃下处理培养细胞,2小时后,CMTC解聚,变为弥散于细胞质内的管蛋白荧光,细胞外形变成更近似圆形或不规则的外形。洗去秋水仙胺,细胞在37℃的新鲜培养基内保温1.6小时后,CMTC又可复现,同时细胞外形又恢复到处理前的成纤维细胞形状。细胞在低温(0—4℃)处理1小时后,CMTC消失;当细胞再放回到37℃下保温16分钟后,CMTC开始恢复,30分...

本工作用我们自制的兔抗管蛋白血清经过间接免疫荧光染色检查,效价达到1:32,显示出我国小儿包皮成纤维细胞(正常二倍体细胞)内微管的特异分布。我们观察到在间期细胞内的微管(CMTC)系由核附近的微管组织中心(MTOC)发出到达细胞的边沿,或终止于质膜下方,或弯曲沿细胞表面平行分布;胞质内的微管纤维有的紧绕核周,有的伸入细胞突起内与主轴方向平行排列。当细胞进入分裂期(M期)时,不仅CMTC解聚,同时有丝分裂器纺锤体微管出现,细胞从扁平形状变成圆的外形。当有丝分裂完成后,纺锤体微管荧光消失,CMTC又逐渐代替而出现,细胞也恢复成为扁平形。CMTC在早G_1期开始出现,此时的子细胞之间由于极间微管束的残迹而呈现出荧光染色的阳性反应,是为中体。用秋水仙胺(0.06微克/毫升)在37℃下处理培养细胞,2小时后,CMTC解聚,变为弥散于细胞质内的管蛋白荧光,细胞外形变成更近似圆形或不规则的外形。洗去秋水仙胺,细胞在37℃的新鲜培养基内保温1.6小时后,CMTC又可复现,同时细胞外形又恢复到处理前的成纤维细胞形状。细胞在低温(0—4℃)处理1小时后,CMTC消失;当细胞再放回到37℃下保温16分钟后,CMTC开始恢复,30分钟后完全恢复。本实验用未免疫的同一家兔血清做对照染色,结果为阴性。本工作改用冷氯仿:甲醇(2:1)液固定细胞,在染色和洗涤时采用非离子性去污剂Triton X-100处理法,微管的荧光染色效果好,背景的非特异荧光减少。

The experiments was conducted on the fibroblasts of human prepuce in vitro todemonstrate the distribution of cytoplasmic microfilaments with Coomassie Blue R250.It has been found that the distribution of the cytoplasmic microfilaments canbe disturbed by the specific inhibitor,Cytochalasin B.We also observed the inhi-bition by Cytochalasin B was reversible.The cytoplasmic microfilaments regainedtheir normal distribution after the Cytochalasin B was removed.This result further confirmed that the staining method...

The experiments was conducted on the fibroblasts of human prepuce in vitro todemonstrate the distribution of cytoplasmic microfilaments with Coomassie Blue R250.It has been found that the distribution of the cytoplasmic microfilaments canbe disturbed by the specific inhibitor,Cytochalasin B.We also observed the inhi-bition by Cytochalasin B was reversible.The cytoplasmic microfilaments regainedtheir normal distribution after the Cytochalasin B was removed.This result further confirmed that the staining method of Coomassie BrilliantBlue R 250 is reliable to demonstrate microfilaments in cultured fibrablasts.

用考马斯蓝显示人包皮成纤维细胞的微丝。使用微丝的特异性抑制剂细胞松弛素 B,对微丝进行抑制处理及恢复实验,进一步肯定了考马斯蓝染色效果的可靠性。

The systematic research on 76 Jinhua pigs of different ages in days shows that the sexual maturity of the male pigs is earlier. The peak of the development of the genital organs generally appears in the period of 60-120 days after birth. The testicle, spermatic duct and external genitalia develop comparatively more quickly and the sexual accessary gland has a tendency of belated maturity. On the 45th day the cavity in the seminiferous tubule begins to form and then on the 60th day it can be seen in every seminiferous...

The systematic research on 76 Jinhua pigs of different ages in days shows that the sexual maturity of the male pigs is earlier. The peak of the development of the genital organs generally appears in the period of 60-120 days after birth. The testicle, spermatic duct and external genitalia develop comparatively more quickly and the sexual accessary gland has a tendency of belated maturity. On the 45th day the cavity in the seminiferous tubule begins to form and then on the 60th day it can be seen in every seminiferous tubule, and sperm appears in 3/7 of the individuals. There is sperm in all the. individuals on the 75th day. The young boar shows the sexual behaviour of mounting on 56.1th±1.16 days, when the. body weight is 8.25 ±0.35kg; the penis outstretches from the foreskin on 67.12th±1.28 clays, their body weight was 10.98 ± 0. 45kg; the first projection of semen appearson 69.2th± 1.33 days, with body weight at 11.3±0.44kg; the first normal sperm appears on 75.35th ± 0.86 day, their body weight at 11.93 ± 0.40kg, judging by the above standard as index of puberty, the puberty of Jinhua pig may be set at approximately 75 days after birth. About 3.5 months after birth the semens of the male pigs reach the normal standard and have the normal fertilization ability. The young boar has reached sexual maturity. During this period, the testosterone content in blood increases quickly and reaches 347.08 ng/100ml on the 120th day. It indicates that there's a close relation between the lesloserone content and the course of development of the sex function. Six months after birth, the quality of the semen has fundamentally reached the level of the adult male pigs and can be considered roughly as the month for first mating.

通过对76头不同日龄金华公猪进行系统研究表明:公猪性成熟较早,生殖器宫发育的增长高峰一般出现在60~120日龄阶段,睾丸、输精管道和外生殖器的发育较快,副性腺有较后熟的趋势。45日龄时睾丸曲细精管部分见有腔裂;60日龄时各个体曲细精管均有腔,3/7的个体已出现精子;75日龄时各个体均有精子。出现性爬跨行为在56.1±1.16日龄,体重8.52±0.35公斤;阴茎伸出包皮为67.12±1.28日龄,体重10.98±0.45公斤;初次射出精液为69.2±1.33日龄,体重11.3±0.44公斤;精液中首次出现形态正常的精子为75.35±0.86日龄,体重11.93±0.40公斤。因此,金华公猪的初情期为75日龄左右。3.5月龄左右精液质量已达常规输精标准,且有正常受精能力,已达到性成熟期。在此期间,血液中睾酮含量增长较快,120日龄的睾酮含量达347.08ng/100ml,显示出与性机能发育进程密切切关。6月龄时精液品质已基本达到成年公猪水平,可作为开始初配的大致月龄。

 
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