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  oxygen radical damage
These results suggest that dexrazoxane may protect myocytes against hypoxia-reoxygenation-induced damage by chelating free or loosely bound iron, thus preventing site-specific iron-based oxygen radical damage.
      
Hydrolyzed dexrazoxane metabolites prevent site-specific iron-based oxygen radical damage by displacing iron from doxorubicin and chelating free and loosely bound iron.
      
Myocardial stunning, defined as a reversible decrease in contractility after ischemia and reperfusion, may be a manifestation of reperfusion injury caused by free oxygen radical damage.
      
In diabetic pregnancy, both diabetes and pregnancy are promoting oxygen radical damage.
      
Because of the physiological importance of IDO in the protection against free oxygen radical damage, a search was conducted to find this enzyme in the eye.
      
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We chose the spectrophotometer method,simple and convenient,to study the antioxidant effects of proanthocyanidins from the friuts of hawthorn(HPA),including the scavenging effects on hydroxyl radicals and superoxide aninon radicals and the inhibition of the peroxidation of polyunsalurated fatty acid.We also studied the protective effects of HPA on hydrogen peroxide induced injury in epithelial cells(here we used NRK cells).The results indicated that...

We chose the spectrophotometer method,simple and convenient,to study the antioxidant effects of proanthocyanidins from the friuts of hawthorn(HPA),including the scavenging effects on hydroxyl radicals and superoxide aninon radicals and the inhibition of the peroxidation of polyunsalurated fatty acid.We also studied the protective effects of HPA on hydrogen peroxide induced injury in epithelial cells(here we used NRK cells).The results indicated that HPA had the antioxidant effects in a concentration dependent manner within the range from 0.1 mg/ml to 1.0 mg/ml.The scavenging effects on oxygen radicals and the inhibition of the peroxidation of polyunsalurated fatty acid were especially notable.The rate of scavenging effects on hydroxyl radicals and superoxide aninon radicals by HPA 1.0 mg/ml were 59.8% and 90.0% respectively,and the rate of protective effects on those cells was 41.96%.While the rate of inhibition of the peroxidation of fatty acid was 91.9% by HPA 0.5 mg/ml.Compared with Vc and the proanthocynidins from grape(GPA),we could see that it had almost the same effects with GPA but significantly higher than Vc.

本文用比色法测定山楂原花色素 (Proanthocyanidinsfromthefruitsofhawthorn ,HPA)的抗氧化作用 ,即对羟自由基、超氧负离子的清除作用以及抗脂质过氧化的作用 ;并且通过细胞学实验验证其对上皮细胞的抗氧化损伤的保护作用。试验表明 :山楂原花色素有很强的抗氧化作用 ,其作用在浓度为 0 .1mg/ml~ 1.0mg/ml之间随着浓度的增大而增强 ,对超氧负离子的清除和抗脂质过氧化作用尤为明显。在浓度为 1mg/ml时 ,对羟自由基和超氧负离子的清除率分别为 5 9 8%和 90 0 % ,对细胞氧化损伤的保护率为 41 96 % ;浓度为 0 5mg/ml时对脂质过氧化的抑制率就达 91 9%。试验同时还比较了葡萄籽原花色素 (Proanthocyanidinsfromthefruitsofgrape,GPA)和VC 的抗氧化作用 ,结果表明 :山楂原花色素与葡萄籽原花色素效应相当 ,并远远高于VC 的效应。

Based on guanine(-H_2O_2-Co~(2+) chemiluminescent system and flow in jection technigae,the protection effect of glutathione(GSH) on guanine oxidation by HO· was studied.The results indicats that GSH can scavenge hydroxyl radical effectively,and the inhibitory rate was greatly relevant to the concentration of GSH 50% of inhibitory was obtained when the concentaration of GSH was 6.5 g/L.This system provides an effective method for the study of DNA anti-oxidation mechanism.

利用鸟嘌呤-过氧化氢-钴(Ⅱ)化学发光体系,结合流动注射技术研究了生物活性物质谷胱甘肽(GSH)对羟自由基氧化鸟嘌呤的保护作用.实验结果表明,GSH明显抑制鸟嘌呤的化学发光,且抑制率与GSH浓度成量效关系,达到50%抑制率的GSH浓度为6.5 mg/L.该方法为进一步研究DNA的抗氧化损伤机制提供了有效的研究手段.

 
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