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包皮
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  prepuce
    Cell culture and analysis of chromosome in fibroblasts from normal fetal skin and adult's prepuce
    正常胎儿皮肤与成人包皮成纤维细胞培养及染色体分析
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    Applied anatomy of the cutaneous blood supply of penis and prepuce
    阴茎皮肤和包皮动脉的应用解剖
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    Applied Anatomy of the Vein of the Penis' Skin and Prepuce
    阴茎皮肤和包皮静脉的应用解剖学
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    Difference of distributive density of calcitonin gene-related peptide immunoreactive positive nerve terminals in the prepuce of penis and frenulum of prepuce and convection of perceptive information in adult human
    成人阴茎包皮包皮系带内降钙素基因相关肽免疫阳性神经末梢分布密度差异与感觉信息的传递
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    Study on a-Actin expression in epidermal cells of prepuce
    包皮表皮细胞中肌动蛋白表达规律研究
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  foreskin
    Physiological variation of androgens and 5α reductase activities in male foreskin at different development stage
    男性不同发育阶段包皮组织中雄激素和5α-还原酶活性变化的研究
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    2. Expression of the recombinant plasmid pIRES2-HPV11E6-EGFPin human dermal fibroblastsHuman foreskin was cut to small pieces and digested with collagenaseand hyaluronidase.
    二、重组质粒 pIRES2-HPV11E6-EGFP 在人真皮成纤维细胞中的表达取临床包皮组织,用含氨苄青霉素和硫酸链霉素的 PBS 反复漂洗后,剪弃脂肪和结缔组织。
短句来源
    CHO, HeLa and chinese fetal foreskin fibroblasts (F-cells)were treated with disodium norcantharidate (10μg/ml) for 6-24hr.
    本实验用去甲斑蝥酸钠10μg/ml处理CHO、HeLa和小儿包皮细胞6~24小时,观察到CHO和HeLa细胞胞质骨架(微管和微丝)结构有比较明显的变化。
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    The content of androgen receptor in cultured foreskin fibroblasts from 40 normal men varing in age from 1.5~60 years was investigated by whole-cell assay with [3H]-methyltrienolone([3H]-R1881).
    为确定国人不同年龄男性靶细胞雄激素受体(AR)的含量及其正常值,我们以氚标记的人工合成的雄激素-[3H]-R1881为配体,采用完整细胞的Scatchard分析及单点分析法测定了40例不同年龄正常男性(从1.5-60岁)包皮成纤维细胞的AR含量。
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    Methods The human foreskin was obtained from 20 patients, and the keratinocytes were isolated by 2.4 U/mL dis-pase and 0.05% tripsin digestion, and then cultured in vitro.
    方法 取2-3岁健康儿童包皮20例,通过2.4 U/mL dispase和0.05%胰酶(tripsin)分离,获得角质形成细胞体外培养。
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  “包皮”译为未确定词的双语例句
    Methods HPV6/11 associated CA was diagnosed by in situ hybridization and the expression and distribution of PCNA, cyclinE, p21 and p27 in 50 HPV 6/11 positive CA specimens and 25 normal human skins (foreskins) were detected by SP immunohistochemistry technique.
    方法 应用原位杂交技术诊断 HPV6/11相关 CA,采用 SP免疫组化染色技术检测 50例 HPV6/11阳性 CA患者皮损、 25例正常人皮肤(包皮)中 PCNA、 cyclinE及 p21、 p27的表达及分布。
短句来源
    Methods Dispase was dispensed to 0.1%, 0.2%, and 0.5% for the digestion of skin pieces at 4 ℃ or 37 ℃ for different time. The digestion time was compared with that of 0.25% trypsin.
    方法 将分离酶配成 0 1%、0 2 %、0 5 % 3个浓度 ,在 4℃或 3 7℃条件下消化头皮或包皮 ,摸索消化的时间 ,并与 0 2 5 %胰蛋白酶消化法进行对比。
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    Results The densities of Meissner corpuscles in ridged bands of both groups were 33.5% and 20.4% respectively, and the difference showed statistical significance ( P= 0.003).
    结果包茎组和包皮过长组皱褶区中触觉小体密度分别为33.5%和20.4%,差异有极显著意义(P=0.003);
短句来源
    Content of Androgen Receptor in Cultured Genital Skin Fibroblasts from 40 Normal Men of Different Age
    国人不同年龄正常男性包皮成纤维细胞雄激素受体的测定
短句来源
    Observation on Meissner's corpuscle in prepuces before and after sexual maturation
    性成熟前后包皮组织中触觉小体的观察
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查询“包皮”译词为用户自定义的双语例句

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  prepuce
Our results showed that, of the 1 000 cases, 64 had the postoperative complications, including 20 cases of edema of prepuce, 15 cases of flap necrosis, 12 hematoma, 9 infections, and 8 cases of fat and clumsy penis.
      
In the first stage a large dorsal apron flap of prepuce skin is developed by a pericoronal incision.
      
A button-hole incision allows the prepuce flap to be reflected to the ventral surface.
      
For proximal cases he recommended his type III technique in which the new urethra is constructed by tubing the dorsal skin of penis and prepuce.
      
The use of the pedicled transverse island flap of the inner prepuce in hypospadias, epispadias and urethral stricture repair
      
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  foreskin
In this study, we investigated the effects of endogenous and exogenous TGF-β on PTHrP production by normal human foreskin keratinocytes.
      
Establishment of proliferating human epithelial cells in vitro from cell suspensions of neonatal foreskin
      
Methods are described for serum-free culture of human epidermal keratinocytes derived from neonatal foreskin tissue.
      
This paper describes the in vitro establishment and chemical treatment of human foreskin epithelial cells which transform the cells to an anchorage-independent state as demonstrated by growth in soft agar.
      
The culture of endothelial cells from porcine pulmonary artery, porcine aorta, porcine coronary arteries, bovine adipose tissue microvessels, human foreskin microvessels, and human umbilical vein is described.
      
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The experiments was conducted on the fibroblasts of human prepuce in vitro todemonstrate the distribution of cytoplasmic microfilaments with Coomassie Blue R250.It has been found that the distribution of the cytoplasmic microfilaments canbe disturbed by the specific inhibitor,Cytochalasin B.We also observed the inhi-bition by Cytochalasin B was reversible.The cytoplasmic microfilaments regainedtheir normal distribution after the Cytochalasin B was removed.This result further confirmed that the staining method...

The experiments was conducted on the fibroblasts of human prepuce in vitro todemonstrate the distribution of cytoplasmic microfilaments with Coomassie Blue R250.It has been found that the distribution of the cytoplasmic microfilaments canbe disturbed by the specific inhibitor,Cytochalasin B.We also observed the inhi-bition by Cytochalasin B was reversible.The cytoplasmic microfilaments regainedtheir normal distribution after the Cytochalasin B was removed.This result further confirmed that the staining method of Coomassie BrilliantBlue R 250 is reliable to demonstrate microfilaments in cultured fibrablasts.

用考马斯蓝显示人包皮成纤维细胞的微丝。使用微丝的特异性抑制剂细胞松弛素 B,对微丝进行抑制处理及恢复实验,进一步肯定了考马斯蓝染色效果的可靠性。

The normal human fibroblast cell line (NHF8) derivd from normal adult foreskin was passaged successfully in cell culture for more than 400 days.The cells were fibro-blast-like and were grown in the cell culture media NO.I with 15% newborn calf serum and were subcultured by trypsinization at a 1 : 2 split ratio. The cell line had a log phase doubling time of 26 h and its growth curve appeared in "s" shape. The mode of the chromosomes of the cell line was 46 and its frequency of sister-chroma -tid exchange (SCE),...

The normal human fibroblast cell line (NHF8) derivd from normal adult foreskin was passaged successfully in cell culture for more than 400 days.The cells were fibro-blast-like and were grown in the cell culture media NO.I with 15% newborn calf serum and were subcultured by trypsinization at a 1 : 2 split ratio. The cell line had a log phase doubling time of 26 h and its growth curve appeared in "s" shape. The mode of the chromosomes of the cell line was 46 and its frequency of sister-chroma -tid exchange (SCE), induced by UV irradiation or [not, was 0.143/per chromosome and 0.144/per chromosome respectively. The level of UV-induced unscheduled DNA synthesis (UDS) was also normal. The results demonstrate that the cell line is normal human diploid fibroblast cell line with normal function of excision repair of DNA damage and can be used as control cells in many studies.

用正常成人包皮组织建立NHF8细胞系在体外长期传代培养达400余天。细胞形态为成纤维样并呈规则的放射状生长。细胞培养于含有15%小牛血清的Ⅰ号培养液中并以1:2比例传代培养。细胞系的群体倍增肘间为26小时,其生长曲线呈“S”形。该细胞系染色体众数为46,自发姐妹染色单体交换率(SCE)为0.144/每染色体,紫外线(UV)诱发SCE率为0.143/每染色体。该细胞系经UV诱发的非合成期DNA修复合成(UDS)水平也是正常的。上述结果表明,NHF8细胞是具有正常DNA损伤修复功能的人二倍体细胞系,可以用于许多研究中作为正常对照细胞。此外,我们还利用该细胞系进行了体外细胞恶性转化及环境诱变物质检测等方面的工作。

The killing effects of disodium norcantharidate on CHO and HeLa cells were much stronger than normal diploid chinese fetal foreskin fibroblasts (F-cells) in vitro. In the presence of the drug at 10μg/ml, the killing rates were 91.8% and 58.3% in CHO and HeLa cells by the end of 48hr., respectively. The F-cells were killed by 7.7% only.Disodium norcantharidate obstructed mitosis of CHO and HeLa cells seriously. The duration of M-phase was delayed, the maximmum MI got up to 60.0% (CHO) and 20.0% (HeLa) after treating...

The killing effects of disodium norcantharidate on CHO and HeLa cells were much stronger than normal diploid chinese fetal foreskin fibroblasts (F-cells) in vitro. In the presence of the drug at 10μg/ml, the killing rates were 91.8% and 58.3% in CHO and HeLa cells by the end of 48hr., respectively. The F-cells were killed by 7.7% only.Disodium norcantharidate obstructed mitosis of CHO and HeLa cells seriously. The duration of M-phase was delayed, the maximmum MI got up to 60.0% (CHO) and 20.0% (HeLa) after treating with the drug (10μg/ml). The chromosomes adhered to each other, and the division of cytoplasm was obstructed, so that the rates of multinuclear offspring cells reached 44% (CHO) and 15.5% (HeLa). The deformation of CHO and HeLa cells resulted in death of these cells.However, the drug obstructed the mitosis of F-cells to a small extent. The maximum MI of F-cells was 5.0%. The rate of multinuclear cells was 1.5%.Disodium norcantharidate obstruct mitosis to different extent, so that resulted in distinctive killing effects on normal diploid cells (F-cells) and abnormal diploid cells (CHO) as well as neoplastic cells (HeLa).

去甲斑蝥酸钠(10μg/ml)处理非正常二倍体细胞(CHO)、人宫颈癌细胞(HeLa),48小时杀伤率分别为91.8%和58.3%;对正常二倍体小儿包皮细胞杀伤率仅7.7%,呈现有区别的杀伤。去甲斑蝥酸钠严重干扰CHO和HeLa细胞的有丝分裂,造成M期阻滞,CHO和HeLa细胞的最高有丝分裂指数(MI)分别高达60.0%和20.0%;染色体粘连成团,胞质分裂受阻,致使细胞形成多核,其多核率高达44.0%(CHO)和15.5%(HeLa)。这种染色体畸形和细胞多核化是CHO细胞死亡增加的主要原因。该药对小儿包皮细胞有丝分裂干扰较小,M期持续时间略有延长,最大有丝分裂指数值5.0%,多核率1.5%(与对照相同),死亡率低。去甲斑蝥酸钠对正常二倍体细胞(小儿包皮细胞)和非正常二倍体细胞(CHO)、肿瘤细胞(HeLa)的有丝分裂干扰程度不同,导致了“区别杀伤”。

 
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