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重叠群
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  contig
     The YAC Contig Construction for Human X Chromosome Xp21. 3-11. 3
     人类X染色体Xp21.3-11.3区YAC重叠群的构建
短句来源
     AYAC Contig Surrounding 2bA3 on Human X Chromosome Linking to DMD YAC Conting
     人X染色体2bA3-YAC克隆重叠群的构建及其与DMD-YAC的串联
短句来源
     Some YAC Contig Construction and Long Range Physical Mapping at Human X Chromosome Xp11.3~21.3
     人类X染色体Xp11.3~21.3区部分YAC重叠群构建及大尺度物理图谱分析
短句来源
     Construction and Large-scale Mapping of a 3. 5 Mb YAC Contig Spanning Human Chromosome Xp 21. 1-21 .3
     人Xp21.1-p21.3上3.5MbYAC重叠群构建及物理图谱分析
短句来源
     Results The whole genome PaP1 was about 90kb, and the acquired biggest contig was 28 249 bp.
     结果 PaP1基因组约为90 000 bp,其中最大重叠群为28 249 bp。
短句来源
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  contigs
     These results allowed us to map these YACs, and finally 6 YAC contigs were obtained in Xp21. 3-11.3,covering about 15 Mb.
     综合上述信息,对这些YAC进行了排序,在Xp21.3-11.3区得到了6个0YAC重叠群,覆盖了约15Mb的范围。
短句来源
     These results allowed us to map these YACs,and finally 6 YAC contigs were obtained in Xp113~213,covering about 153 Mb.
     综合上述信息,对这些YAC进行了排序,在Xp11.3~21.3区得到了6个YAC重叠群,总共覆盖了约15.3Mb的范围。
短句来源
     ⑵By searching the Bos_Taurus_WGS_Trace database with BLASTN program, we got many Contigs for RAPD marker S139-921. Using DNAMAN 5.0 software, we jointed these Contigs and extended the RAPD fragment S139-921 from 921 bp to 2141 bp in two orientations. The left end was extended 562 bp, and the right end was extended 658 bp.
     初步推测该标记对应奶牛基因组中的一个LINE重复元件。 ⑵ 用BLASTN程序在Bos_Taurus_WGS_Trace数据库中搜索RAPD 标记S139-921片段的重叠群序列(Contigs),然后用DNAMAN 5.0软件进行拼接,使RAPD标记S139-921向右端延伸了658 bp,向左端延伸了562 bp,得到一个包含整个RAPD标记片断(921 bp)的序列,全长2141 bp。
短句来源
     The 2098 valid sequences formed 168 contigs and 1693 singlet,which represented the total 1861 unigenes;
     进一步对这2098条组装分析,形成168个重叠群(Contig)和1693个单拷贝EST(Singlet),代表了共计1861条独立基因(Unigene);
短句来源
     4. All sequences were assembled and clustered into 1,492 contigs and 3,545 singletons by the EST Analysis Pipeline System? Program and total 5,037 Unigenes were obtained, among which 73.9% are singletons.
     4.通过EST Analysis Pipeline SystemTM Program对9,664条有效EST进行序列拼接和组装,得到1,492个重叠群(Contigs)和3,545个单拷贝EST(Singletons),共计获得5,037个独立基因(Unigenes),其中单拷贝基因占到73.9%。
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  “重叠群”译为未确定词的双语例句
     13E3, 32G8 were selected to subclone.
     构建了由13E3、32G8、186G20组成的重叠群
短句来源
     (3) The primers that using for isolating exon 2 and exon 3 of SLA-1 and SLA-2 in Wuzhishan pig were designed according to the conting sequences of pig EST.
     (3)依据猪EST所构建的EST重叠群,设计的猪特异引物分离并鉴定五指山猪群体中SLA-1,SLA-2基因第二和第三外显子的多态性。
短句来源
     This EST was mapped to 9q34 due to 95.6% identity with one genomic sequence (GenBank: AC002295).
     此EST与9q34上的2个gDNA构成的连续重叠群序列(71094hp)95.6%一致。
短句来源
     The niche overlaps of 11 species of gamasid mites on 25 species of small mammals, host resource series, were measured by means of Colwell-Futuyma's model, and then all species of gamasid mites were divided into seven niche-overlapping groups in combination with fuzzy clustering analysis.
     本文应用Colwell─Futuyma模型对云南西部11种重要革螨在25种小兽宿主资源上的生态位重叠进行了测定,在此基础上结合模糊聚类分析,11种革螨被划分为7个生态位重叠群
短句来源
     Z which involve in melanin, tyrosinase related protein 1 gene (TYRPI) and dermal melanin inhibitor gene (ID). The genomic structure of TYRP1 was determined and its correlation between melanin accumulation and TYRP1 expression was studied.
     利用构建的BAC文库对Z染色体上黑色素相关基因酪氨酸酶相关蛋白1基因(TYRP1)的基因结构以及该基因的表达与黑色素沉积的关系进行了研究,同时构建了位于Z染色体上缺乏标记区段的表皮黑色素抑制因子基因(ID)的BAC重叠群
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  contig
The positions of the NotI-STS markers and their order on the chromosome were determined by a combination of RH-mapping (our data), contig mapping, cytogenetic mapping, and in silico mapping.
      
Three genes (POLR2J1, POLR2J2, and POLR2J3) form a cluster of total length of 214 530 bp in the genetic locus 7q22.1 on the long arm of chromosome 7 (contig NT_007933).
      
The fourth gene (POLR2J4, 31 040 bp) was localized in the cytogenetic locus 7p13 of the short arm of chromosome 7 (contig NT_007819).
      
A BAC contig encompassing the mutation locus, which consists of T6P5, T7M23, T12A21, T8L6 and T18A18, was identified by Southern hybridization with the BAC ends as probes.
      
Based on a contig assembled from homologous ESTs, a 1 256-bp humanMSRA cDNA was cloned from several human cDNA libraries.
      
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  contigs
These libraries played an important role in sequencing of genomes of human, mouse, and other organisms as an instrument linking molecular biological and cytogenetic data via construction of contigs and their localization on the chromosomes.
      
A total of 28941 ESTs were sequenced from five 5'-directed non-normalized cDNA libraries, which were assembled into 2212 contigs and 5642 singlets using CAP3.
      
By the electric hybridization walking, we obtained two full-length contigs with a length of 4632 and 2235 bp respectively.
      
Revealed by bioinformatics analysis, 1162 out of 21 954 ESTs contained microsatellites and cluster analysis indicated that 984 of these ESTs fell into 112 contigs, while the other 178 ESTs were singletons.
      
A total of 59 contigs consisting of 419 BAC clones including 5 single-clones were physically aligned on rice chromosome 12 with the largest BAC contig covering 855 kb.
      
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AYAC contig on human Xp2 1.1一21.2 surrounding the DXS I66 site are analyzed using twostepAl uPCR fingerprinting method,which link to DMD YAC contig constructed before.The overlappingresuIts are also confirmed by YAC end probe cross hybridization and the DMDYAC contig are extended atleast to DXS 1 6 6 site covering 3. 5 Mb. This work facilitates further mapping analysis and clon ing longupstream transtrictional elements of DMD gene and unk nown genes in this region;

用Alu-PCR指纹图谱法分析了人Xp21.1一21.2上一系列的YAC克隆,发现其中的两个YAC克隆构成包含DXS166位点的重叠群,而且这一重叠群与以前构建的包含DMD基因全顺序的YAC重叠群相连接,YAC克隆未端探针杂交证实了这一重叠,使这一YAC重叠群至少延伸至DXS166位点,形成一个跨度为3.5Mb的YAC大重叠群。这一工作为这一区域进一步的图谱分析及DMD基因的5′远端调控和该区域未知基因克隆奠定了基础。

By using the theory and methods of ecological niche in mordern ecology, the niche breadth and niche overlap of 11 dominant species of gamasid mites, namely laelaps echidninus, L. nuttalli, L.algericus, L. jettmari, L. chini, Haemolaelaps glasgowi, Proctolaelaps pygmaeus, Haemogamasus oliviformis,Euiaelaps shanghaiensis, Ornithonyssus bacoti, and Hirstionyssus sunci, in a resource-series formed by 25 species of hosts, all are small mammals, from 12 counties in Western Yunnan were studied. The index of Levins'...

By using the theory and methods of ecological niche in mordern ecology, the niche breadth and niche overlap of 11 dominant species of gamasid mites, namely laelaps echidninus, L. nuttalli, L.algericus, L. jettmari, L. chini, Haemolaelaps glasgowi, Proctolaelaps pygmaeus, Haemogamasus oliviformis,Euiaelaps shanghaiensis, Ornithonyssus bacoti, and Hirstionyssus sunci, in a resource-series formed by 25 species of hosts, all are small mammals, from 12 counties in Western Yunnan were studied. The index of Levins' niche breadth and the modal of Colwell-Futuyma's niche overlap are measured for each species of gamasid mites. On the basis of measuring the niche overlaps, the niche overlaping groups for all gamasid mites were classified by fuzzy clustering analysis. The results showed that: 1) the niche breadths of Laelaps nuttalli and Laelaps echidninus were the widest, while Laelasp algericus and Laelasp chini the narrowest, 2) seven niche overlaping groups could be classified from the phenogram of fuzzy clustering analysis, 3) a positive correlation was found between gamasid mites'niche breadths and their host ranges with 0.8501 correlation coefficient(P<0.01). It was suggested that the niche breadth could be used as an index for evaluating the host specificity of gamasid mites and other sctoparasites.

本文应用现代生态学中生态位的理论和方法,对云南西部11种主要革螨在25种小兽宿主资源上的生态位宽度和生态位重叠进行了研究。生态位宽度用Levins模型测定;生态位重叠用Colwell-Futuyma模型测定,并在此基础上用模糊聚类分析进行了生态位重叠群的划分。研究结果表明:①11种革螨中,纳氏厉螨及毒厉螨的生态位最宽,阿尔及利亚厉螨及金氏厉螨的生态位最窄;②模糊聚类分析将11种革螨划分为7个生态位重叠群;③革螨生态位宽度与其所寄生的宿主范围呈正相关(r=0.8501,P<0.01),生态位宽度可作为评价革螨等体外寄生虫宿主特异性的定量指标。

A YAC contig on human chromosome Xp21 .1-p21 .2 region surrounding the DXS 166 locus was generated and linked up with the DMD YAC contig eonstructed before, using the two-step Alu-PCR fingerprinting. The overlapping results were also confirmed by cross hybridization with YAC end probes, and the DMD YAC contig was extended at least to the DXS166 locus covering 3.5 Mb. Based on this contig, a complete long-range restriction enzyme map spanning the Xp21.1-p21 .3region was also generated which reveals the relationship...

A YAC contig on human chromosome Xp21 .1-p21 .2 region surrounding the DXS 166 locus was generated and linked up with the DMD YAC contig eonstructed before, using the two-step Alu-PCR fingerprinting. The overlapping results were also confirmed by cross hybridization with YAC end probes, and the DMD YAC contig was extended at least to the DXS166 locus covering 3.5 Mb. Based on this contig, a complete long-range restriction enzyme map spanning the Xp21.1-p21 .3region was also generated which reveals the relationship between the physical location of DXS166 locus and DMD gene. This work should facilitate further maping analysis and cloning of remote upstream transcriptional elemente of the DMD gene, as well as unknown genes in this region.

用Alu-PCR指纹图谱法分析了人Xp21.1-p21.3上一系列的酵母人工染色体(yeastartificialchromosome,YAC)克隆,发现其中的两个YAC克隆构成包含DXS166位点的重叠群,而且这一重叠群与以前构建的包含DMD基因全序列的YAC重叠群相连接,YAC克隆末端探针交叉杂交证实了这一重叠,使这一YAC重叠群至少延伸至DXS166位点,形成一个跨度为3.5Mb的YAC重叠群。基于这些重叠的YAC克隆绘制了这一区域的大尺度限制酶切图谱,并在这一图谱上定位了DXS166位点,从而确定了DXS166位点与DMD基因的物理关系。这一工作为DMD基因的5'远端调控作用研究及该区域未知基因的克隆奠定了基础。

 
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