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Osmium impregnation and three marker enzymes (nicotinamide adenine dinucleotide 应用锇酸浸染法和酶的细胞化学方法,观察精子细胞高尔基体的细胞化学反应。实验表明,嗜锇反应以及NADP酶、TPP酶和CMP酶三种酶细胞化学反应是一组很好的高尔基体标志细胞化学反应,这组方法对进一步研究高尔基体的结构与功能有一定价值。 The results of drug susceptibility in vitro test, using "tubes method" invented by Zierdt et al, in 1983, indicat that Blastocystis hominis is not sensitive to albendazole, levamisole, mebendazole, allitridi, genfamyeini sulfas and neomyclin, but is sensitiv to metrondazole, mefloquine, chininoforn, chloramphenicolm and doxycyclin hydrochloridum. The effect of metroiazole on B. hominis is studied with SEM and TEM. Observations on the fine structures reveal that metronidazole obviously destroys the endomembrane... The results of drug susceptibility in vitro test, using "tubes method" invented by Zierdt et al, in 1983, indicat that Blastocystis hominis is not sensitive to albendazole, levamisole, mebendazole, allitridi, genfamyeini sulfas and neomyclin, but is sensitiv to metrondazole, mefloquine, chininoforn, chloramphenicolm and doxycyclin hydrochloridum. The effect of metroiazole on B. hominis is studied with SEM and TEM. Observations on the fine structures reveal that metronidazole obviously destroys the endomembrane system—golgi body, vecicoles, endoplasmic reticulum and outer membrane of nucleus. And as a result, all these lead to destruction of the outer cell membrane and mitochondria. On the other hand, metronidazole destroys the filamentous layer of the parasite, forming honeycome-like caves on the surface of B.hominis. 在体外,11种药物对人芽囊原虫作用的结果表明:人芽囊原虫对抗原虫药物灭滴灵、甲氟喹、喹碘仿敏感,对大蒜素不敏感;对抗蠕虫药物阿苯达唑、左旋咪唑、甲苯咪唑不敏感;对抗生素氯霉素和土霉素敏感,对庆大霉素和新霉素不敏感。灭滴灵作用虫体,扫描电镜观察,表面有蜂窝状凹陷及裂逢,透射电镜观察,细胞质内质网、高尔基体、细胞内膜及细胞核外膜破损,虫体细胞质出现空泡化和颗粒化。 The role of virus movement protein in the spread of infection in plants is reviewed. The subjectsinclude: movement protein-nucleic acid complex mediates viral cell-to-cell movement; the roles of cellularorganelles or cellular factors on viral spread, including endoplasmic reticulum, cytoskeleton, pectin methylesterase, inclusion bodies, and β-1, 3-glucanase; and regulation of the activity of movement protein. 综述了病毒在植物寄主内扩散中的运动蛋白的作用。由病毒基因组编码的运动蛋白与病毒核酸形成运动蛋白核酸复合物,介导病毒扩散。在病毒复制与扩散过程中,运动蛋白与宿主细胞内质网、高尔基体、细胞骨架、胞间连丝发生作用,并受细胞果胶甲基脂酶、包含体、β-1,3-葡聚糖酶、磷酸化等因素的影响,形成了植物体内遗传物质系统性运输的一个模式。
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