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平面培养
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     In this paper, to use Cytodex-3 as microcarrier, we established Three-dimensional (3D) culture of rat WB-F344 cells with rotary cell culture system (RCCS) to simulate microgravity environment.
     本文以大鼠肝干细胞系WB-F344为实验材料,采用RCCS系统模拟微重力效应,并以Cytodex-3为微载体对细胞进行培养(以平面培养的细胞为对照),分析微重力三维培养条件下细胞形态、增殖、细胞内部显微结构及细胞粘附分子表达情况;
短句来源
     2D static culture group: The cells were inoculated directly in a plane culture flask for static culture.
     二维静态培养组:细胞直接接种于平面培养瓶静态培养。
短句来源
     2D dynamic culture group: The cells were inoculated directly in a culture flask in RCCS for a dynamic culture under microgravity.
     二维动态培养组:细胞直接接种于平面培养瓶,置于旋转式细胞培养系统,微重力条件下动态诱导。
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     The detected mRNA expression of adhesive molecules by RT-PCR demonstrated that on 3D culture and monolayer culture,although various adhesive molecules were expressed,Fn and integrin-β1 up-regulated more apparently and integrin-α 5 also elevated slightly on 3D culture.
     三维培养和平面培养下,各种粘附分子均可表达,而三维培养条件下纤粘连蛋白(Fn)和整合素-β1(integrin-β1)mRNA的表达明显上调,整合素-α5的表达也有所增加。
短句来源
     (2) The significant differences in the expression of E-cad and drug susceptibility to ADM between 2D and 3D cells decreased after anti-adherence treatment.
     2. 平面培养与立体培养相比,在粘附分子E-cad的表达及对化疗药物ADM的药物敏感性方面存在非常显著的差异,经相应抗粘附处理后差异性明显缩小。
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     MCF-7 monolayer cells were used as control.
     以平面培养细胞做对照。
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     The program to educate the person with ability for TFT-LCD technology
     “平面液晶显示技术”专业人才培养的规划和设想
     On the Cultivation of Esthetic Judgment
     审美能力的培养
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     Then how?
     如何培养?
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     From Plan to Section
     从平面到剖面
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  plate culture
Endothelial permeability to horseradish peroxidase (HRP) was assayed in multi-well plate culture.
      
Attached cells are able to grow and fix nitrogen in both liquid and plate culture.
      
Square-plate culture method allows detection of differential gene expression and screening of novel, region-specific genes in As
      
In this study, we developed the square-plate culture method, a novel culture method that enabled the extraction of mRNA samples from the three regions and analyzed the differential gene expression of the A.
      
stercoralis were isolated from cultures of dog's feces using agar plate culture method.
      
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The pathogen solution from PDA slate medium mixed with sweetpotato cuttings was transplanted into vermiculits. Observation was done 25-30 days after the transplanting. The yellowish and blackness leaves of roots were scaled. The activity of peroxide was tested on inoculated plants. The results showed that big difference existed between varieties with resistance or susceptibility, and the activity of susceptible varieties was found to be higher than that of varieties with high resistance. Peroxide activity could...

The pathogen solution from PDA slate medium mixed with sweetpotato cuttings was transplanted into vermiculits. Observation was done 25-30 days after the transplanting. The yellowish and blackness leaves of roots were scaled. The activity of peroxide was tested on inoculated plants. The results showed that big difference existed between varieties with resistance or susceptibility, and the activity of susceptible varieties was found to be higher than that of varieties with high resistance. Peroxide activity could be used as an indirect index to evaluate the root rot resistance.

将PDA 平面培养的甘薯根腐病菌研磨成浆,按一定比例加入蛭石中,接菌最适浓度为每200 g蛭石加1/ 2 培养皿菌浆,随后栽入薯苗,在25 ℃下培养25 ~30 d 后检查。以薯苗叶片枯黄程度及根部黑根死根多少划分病级,以病级平均数确定品种抗性,同时对抗感品种接种根腐病菌后过氧化物酶活性进行测定。试验证明,上述方法可以简便、快速、准确地测定品种对根腐病的抗性,同时也证明过氧化物酶活性作为量化分级标准的可行性。

Objective To establish the isolation and primary cultivation system of porcine hepatocytes in accordance with the requirement of hybrid bioartificial liver support system (HBLSS) of cell in vitro .Methods Porcine hepatocytes were isolated with digestive method of collagenase perfusion with the improved Seglen method. Morphological changes of the hepatocytes were observed.Results The average yield of hepatocytes was 2×10 10 cells per pig and the average viability was 92 5%.Conclusion The digestive...

Objective To establish the isolation and primary cultivation system of porcine hepatocytes in accordance with the requirement of hybrid bioartificial liver support system (HBLSS) of cell in vitro .Methods Porcine hepatocytes were isolated with digestive method of collagenase perfusion with the improved Seglen method. Morphological changes of the hepatocytes were observed.Results The average yield of hepatocytes was 2×10 10 cells per pig and the average viability was 92 5%.Conclusion The digestive method of collagenase perfusion is the basic approach for obtaining a mass of hepatocytes with high viability. Porcine hepatocytes are the prime cells source for HBLSS.

目的 :建立稳定的猪肝细胞的分离与原代培养体系 ,满足生物人工肝体外支持系统对肝细胞的需求。方法 :应用改良 Seglen法进行肝脏原位胶原酶灌流分离猪肝细胞 ,进行平面培养 ,动态观察细胞生长过程中的形态结构变化。结果 :平均每只猪可获得 2× 1 0 1 0个肝细胞 ,细胞活率达到 92 .5 %。在平面培养过程中维持了正常肝细胞的形态。结论 :原位胶原酶灌流法是获得大量高活率的猪肝细胞的首选方法 ,猪肝细胞原代分离培养是目前解决组合型生物人工肝脏 (HBL SS)应用中细胞来源问题的主要途径

Objective To investigate the roles of PI3K pathway in multicellular resistance of lung adenocarcinoma A549 cells. Methods Three dimensional culture of A549 cells was conducted using rotatable culture bottle and desk top water bath shaker. Western blot, in situ cell apoptosis detection, MTT, and blood cell counter were employed to detect the expressions of Akt, Bcl 2, and Bad, the apoptotic rate, and the susceptibility of apoptosis to adriamycin (ADM) of A549 cells under different culture conditions....

Objective To investigate the roles of PI3K pathway in multicellular resistance of lung adenocarcinoma A549 cells. Methods Three dimensional culture of A549 cells was conducted using rotatable culture bottle and desk top water bath shaker. Western blot, in situ cell apoptosis detection, MTT, and blood cell counter were employed to detect the expressions of Akt, Bcl 2, and Bad, the apoptotic rate, and the susceptibility of apoptosis to adriamycin (ADM) of A549 cells under different culture conditions. Results Compared with cells cultured in two dimensional culture system (2 D system), higher Akt and Bcl 2 expressions, lower Bad expression and apoptotic rate, and decreased susceptibility to ADM were found in cells cultured in three dimensional culture system (3 D system). The differences diminished significantly after blocking the PI3K pathway. Conclusion PI3K pathway has effect on the multicellular resistance of lung adenocarcinoma A549 cells via inhibiting cell apoptosis. Blocking this pathway may revert the resistance.

目的 探讨PI3K信号通路在肿瘤群集耐药中的作用。方法 采用旋转培养瓶结合台式水浴恒温振荡器旋转培养的方法 ,进行肺腺癌A5 49细胞体外立体培养 ,通过Westernblot、原位细胞凋亡检测、四唑盐 (MTT)比色法及血球计数器计数法检测Akt、Bcl 2、Bad的表达、细胞凋亡率及对阿霉素 (ADM )敏感性。结果 ①立体培养细胞存在Akt的高表达 ;②同平面培养比较 ,立体培养细胞存在Bcl 2高表达 ,Bad低表达 ,细胞凋亡率低 ,对ADM敏感性低的特征 ,阻断PI3K信号通路后 ,两者差异明显缩小。结论 PI3K信号通路可通过抑制细胞凋亡在肺腺癌A5 49细胞群集耐药中发挥作用 ,阻断PI3K信号通路可能逆转该耐药现象

 
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