助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   底层培养 的翻译结果: 查询用时:0.014秒
图标索引 在分类学科中查询
所有学科
更多类别查询

图标索引 历史查询
 

底层培养
相关语句
  相似匹配句对
     On the Cultivation of Esthetic Judgment
     审美能力的培养
短句来源
     How to cultivate?
     如何培养?
短句来源
     The Establishment of a Model of Culturing Normal Human Oral Mucosal Basal Cells
     口腔粘膜基底层上皮细胞体外培养模型的建立
短句来源
     BOTTOM CURRENT IN TAIWAN STRAIT
     台湾海峡的底层
短句来源
查询“底层培养”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  subculturing
2,4-Dichlorophenoxyacetic acid (2,4-D) proved to be the most suitable auxin for the induction and subculturing of morphogenic callus.
      
In this study, a comparative analysis of cultured mesenchymal stromal cells from the rat bone marrow at the early and late stages of subculturing has been performed using molecular genetic and cytological methods.
      
The resuscitation of lyophilized bacteria by subculturing them in rich nutrient media, such as skim milk, led to the restoration of their physiological activity, including the effective antimicrobial spectrum
      
typhimurium TA100 induced by hexylresorcinol has been found to be the same as that of the S-and R-type salmonella variants obtained by the routine subculturing method.
      
Periodical subculturing on solid and liquid media was used for maintenance.
      
更多          


Objective To establish a method of culturing endothelial cells (EC) from human vascular malformation. Methods Venous malformation specimens obtained from the patient undergoing oral surgery were plated into glass, plastic and gelatin coated dishes. Pure cultures of human vascular malformation endothelial cells (VMEC) were isolated by the ways of discarding the tissues at early stage of primary culture, scraping and trypsinizing. Morphological characteristics were studied under phase contrast microscope and...

Objective To establish a method of culturing endothelial cells (EC) from human vascular malformation. Methods Venous malformation specimens obtained from the patient undergoing oral surgery were plated into glass, plastic and gelatin coated dishes. Pure cultures of human vascular malformation endothelial cells (VMEC) were isolated by the ways of discarding the tissues at early stage of primary culture, scraping and trypsinizing. Morphological characteristics were studied under phase contrast microscope and electron microscope (EM), and determined by immunohistochemistry. Results The cells were pure and could be maintained in culture up to 4~5 passages, or 40~50 days. VMEC formed contact inhibited "cobblestone" monolayer on glass and plastic , and capillary like"tubes" on gelatin. EM revealed that there were Weibel Palade bodies in the culture cells. The cells showed positive staining for CD 34 , vWF, and negative for α SMA. Conclusions The culture technique for growing VMEC has been established. And these cells can provide a useful tool for studying biological characteristics of human vascular malformation in vitro.

目的 建立一个稳定的人血管畸形内皮细胞体外培养体系。方法 取人静脉畸形组织块接种于铺有明胶底层的培养瓶中进行原代及传代培养。通过早期去除组织块、机械刮除及酶消化法纯化内皮细胞。对培养的细胞进行形态学观察及免疫组化染色等一系列细胞定性研究。结果 获取的内皮细胞可连续传代 4~ 5代 ,成活 4 0~ 5 0d ;生长于玻璃、塑料上的细胞呈铺路石状 ;生长于明胶底层上的细胞可形成毛细血管样结构 ;电镜下可见细胞具有Weibel Palade小体特征性超微结构 ;CD3 4 及vWF免疫组化染色阳性 ,α SMA染色阴性。结论 应用此培养方法可获得纯化的血管畸形内皮细胞 ,且细胞可连续传代培养。培养的血管畸形内皮细胞可用于体外研究血管畸形的生物学行为。

Aim: To developed a procedure for the isolation and culture of vascular smooth muscle cells from human cerebral arteriovenous malformation. Methods: The surgical specimens were digested with collagenase type IV . The cell population then was placed on the dishes percoated with gelatin for primary culture. The cell line derived was then analyzed by cellular morphology, immunochemistry and electromicroscopy. Results: The first growing cell colonies appeared 6 to 8 days after initial seeding. The cell was confirmed...

Aim: To developed a procedure for the isolation and culture of vascular smooth muscle cells from human cerebral arteriovenous malformation. Methods: The surgical specimens were digested with collagenase type IV . The cell population then was placed on the dishes percoated with gelatin for primary culture. The cell line derived was then analyzed by cellular morphology, immunochemistry and electromicroscopy. Results: The first growing cell colonies appeared 6 to 8 days after initial seeding. The cell was confirmed to be VSMC by immunochemistry analysis and the morphological and ultracstructural characteristics were consistent with that of VSMC.The cells still grows well after 18 generations. Conclusion: A purified VSMC cell line can be developed by collagenase digest assay and the cells can be used as a cellular model in vitro.

目的:建立一个稳定的人脑动静脉畸形血管平滑肌细胞的培养体系。方法:取手术切除的人脑动静脉畸形组织块以酶消化法接种于铺有胶原底层的培养瓶中进行原代及传代培养,并对其进行形态学观察、免疫组化染色及透射电镜等一系列细胞定性研究。结果:接种后6-8 d后可见少量细胞贴壁,所获培养细胞经免疫组化、透射电镜鉴定证实为血管平滑肌细胞,而且细胞纯度较高,细胞可以连续传代18代。结论:应用酶消化法可获得纯化的血管平滑肌细胞,细胞可以连续传代培养。培养动静脉畸形血管平滑肌细胞可应用于体外作进一步的深入研究。

 
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关底层培养的内容
在知识搜索中查有关底层培养的内容
在数字搜索中查有关底层培养的内容
在概念知识元中查有关底层培养的内容
在学术趋势中查有关底层培养的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社