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葡萄卷叶
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  grape leaf roll
     Abstraction and Analysis on dsRNA of Grape Leaf Roll Virus
     葡萄卷叶病病毒双链RNA(dsRNA)提纯分析研究
短句来源
  grapevine leaf roll
     PURIFICATION, SEROLOGY AND DETECTION OF GRAPEVINE LEAF ROLL VIRUS
     葡萄卷叶病毒的纯化、血清学研究及其在脱毒组培苗检测中的应用
短句来源
     CLONING, SEQUENCING AND EXPRESSING OF COAT PROTEIN GENE OF GRAPEVINE LEAF ROLL ASSOCIATED CLOSTEROVIRUS
     葡萄卷叶病毒外壳蛋白基因的克隆、序列分析及其在大肠杆菌中的表达
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  “葡萄卷叶”译为未确定词的双语例句
     Grapevine leafroll associated virus-2 and -3 are two important agents of grapevine leafroll disease.
     葡萄卷叶伴随病毒(Grapevine leafroll-associated virus,GLRaV)-2和GLRaV-3是引起葡萄卷叶病(Grapevine leafroll disease,GLD)发生的两种比较重要的病毒。
短句来源
     Comparative Studies on DAS -ELISA,RT -PCR and IC -RT -PCR for Detecting Grapevine leafroll-associated virus-3
     DAS-ELISA、RT-PCR和IC-RT-PCR检测葡萄卷叶病毒Ⅲ的比较研究
短句来源
     RT-PCR Detection of Grapevine Leafroll Associated Virus 3 with Different Primer Sets
     葡萄卷叶病毒3不同引物RT-PCR检测效果研究
     Comparision of three ELISA methods for detection of GLRaV and GFLV
     三种ELISA方法检测葡萄卷叶病毒(GLRaV)和扇叶病毒(GFLV)的比较
短句来源
     Studies on RT-PCR Detection Technology of GLRaV Ⅲ
     葡萄卷叶病毒RT-PCR检测技术研究
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  相似匹配句对
     PROGRESS OF RESEARCH ON GRAPEVINE LEAF-ROLL DISEASE
     葡萄病的研究进展
短句来源
     Detection of Grapevine Leafroll Virus with ELISA
     葡萄病的ELISA检测
短句来源
     Identification And Detection of GLRaV And Reseach On Techniques of Gapevine Viruses Elimination
     葡萄病的鉴定及脱毒技术研究
短句来源
     Detection of GLRaV by RT-PCR
     葡萄病毒RT-PCR检测技术
短句来源
     Gene clone and nucleotid sequence analysis of grapevine leafroll virus 3
     葡萄伴随病毒基因的克隆及序列分析
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Three years′(1990~1992)sampling investigations of the grape-growing

1990~1992年作者对福建省葡萄产区进行了抽样调查,发现该省约31%的果园有葡萄扇叶病(GFLV)和葡萄卷叶病(GLRV)。在调查的11300株葡萄中(602亩),GFLD株发病率为13.27%,造成葡萄减产27.2%;GLRV株发病率为7.3%,减产21.5%。病株表现为不同程度的种性退化,生长赢弱、停滞,果实品质下降,果粒成熟不齐。

Closterovirus-like particles associated with grapevine leafroll disease were purified from stem phloem tissue by differential centrifugation and cesium sulphate-sucrose density gradient centrifugation. The particles were between 660-2000nm long. Most of them were about 1400nm long and also decorated by the antiserum against GLRV NY-1 isolate. The purified preparation was tested for their serological relatedness in indirect ELISA. The results indicated that these closterovirus-like particles reacted with serotype...

Closterovirus-like particles associated with grapevine leafroll disease were purified from stem phloem tissue by differential centrifugation and cesium sulphate-sucrose density gradient centrifugation. The particles were between 660-2000nm long. Most of them were about 1400nm long and also decorated by the antiserum against GLRV NY-1 isolate. The purified preparation was tested for their serological relatedness in indirect ELISA. The results indicated that these closterovirus-like particles reacted with serotype Ⅱ, Ⅲ and Ⅳ of GLRV. The antiserum to NY-1 (type Ⅲ) reacted more strongly than type Ⅳ (to VA-4) and type Ⅱ. (to 1800nm). In sodium dodecyl sulfate immunodiffusion test, the extract of diseased petiole reacted with serotypeⅢ, Ⅳ and Ⅱ. It is possible to be infected by 2 or 3 closterovirus-like particles in China. We have made antiserum to the purfied closterovivus-like GLRV and set up a PAS-ELISA to detect GLRV-free grapevine plantelets. We have obtaimed 21 varieties of GLRV-free and GFLV-free grapevine. They shown high quality and quantity in field test

将具有典型葡萄卷叶病(Grapevine leafroll diseas,GLRD)症状的葡萄组织,经差速和硫酸铯—蔗糖密度梯度离心,提纯了GLRV,并制备了兔抗血清。电镜下可观察到长度从600~2000nm的线形病毒颗粒,其中以1400nm左右为主。免疫电镜结果表明线形病毒颗粒能被美国的NY-1分离株抗血清(Ⅲ型)所修饰。在间接ELISA中提纯制品与GLRV的Ⅲ、Ⅳ、Ⅱ型抗血清均能产生免疫反应。与Ⅲ型抗血清产生较强的免疫反应,Ⅳ型次之,Ⅱ型最弱。在SDS-免疫双扩散实验中病组织韧皮部粗提液与GLRV的Ⅲ,Ⅳ、Ⅱ型抗血清均产生免疫沉淀线。从而推测我国葡萄园内的葡萄卷叶病很可能由2种或3种卷叶病毒感染所致.采用A蛋白夹心酶联免疫吸附试验(PAS-ELISA)检测葡萄试管苗,Ⅲ型抗血清和自制抗血清的平行测试结果基本相符,共获得11个生食葡萄和10个山葡萄品种的脱葡萄卷叶病毒和扇叶病毒的组培苗,扩繁后田间试种表现出良好的农艺性状。

A pair of primers were designed and synthesized based on the nucleotide sequence of coat protein of GLRav 3 from America.By IC RT PCR method,the expected size PCR products were amplified.This product was cloned into vector PGEM3Zf,then translated into E.Coli BL2l,and the recombinant plasmid has been obtained.The nucleotid sequence analysis of PCR products showed 94.1% of the nucleotid sequence is identical with nucleotid sequence of coat protein of GLRaV 3 from America.

根据葡萄卷叶伴随病毒 美国分离物的 GLRa V- 3CP基因序列 ,设计并合成了 1对该病毒的 PCR引物 ,利用 IC- RT- PCR成功地检查到合适大小的 PCR产物 ;同时将PCR产物克隆到中间载体 PGEM- 3Zf上 ,转化大肠杆菌 DH5α菌株 ,筛选阳性克隆。通过双酶切以及序列分析表明 ,扩增样品与美国分离物的同源性为 94.1 %。

 
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