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缺氧培养     
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  hypoxic culture
     The study on the PC12 cell apoptosis and its mechanism after hypoxic culture.
     PC12细胞缺氧培养后细胞凋亡及其机制的研究
短句来源
     Methods The apoptosis of PC12 cells in different time points after hypoxic culture was observed by TUNEL techniques and the expression of DNA damage related genes detected by flow cytometry techniques.
     方法 采用末端标记法 ,结合流式细胞术观察PC12细胞缺氧培养不同时间点细胞凋亡现象 ,以及DNA损伤相关基因表达的改变。
短句来源
     Results:Apoptosis of cells cultured in hypoxic condition were increased in 8 and 16 h of hypoxic exposure compared with 0 h ( P <0.01), at the same time, PCNA expressions decreased ( P <0.01), VEGF expressions were negative during 0, 8, 16 h of hypoxic culture.
     结果 :缺氧培养 8至 1 6小时后 ,凋亡细胞增加 (与缺氧 0小时相比 ,P <0 .0 1 ) ,PCNA表达下降 (与缺氧 0小时相比 ,P <0 .0 1 )、VEGF无表达 ;
短句来源
     Forty-eight h after hypoxic culture (5% CO2 + 95% N2), bcl-xL mRNA and its protein expression in all three groups was detected by RT-PCR and immunochem-istry respectively. Apoptosis index was measured by TUNEL.
     缺氧培养(5%CO2+95%N2)48 h后,采用逆转录-聚合酶链反应(RT-PCR)和免疫细胞化学染色法半定量检测bcl-xL mRNA及蛋白表达,末端DNA转移酶dUTP缺口末端标记法 (TUNNEL)法检测细胞凋亡指数。
短句来源
     Conclusions:For SKOV 3ipl cells ,apoptosis and decrease of PCNA expression were induced during 8 16 h hypoxic culture, but after 24 h exposure ,anti apoptosis and cell proliferation inhibition developed .
     结论 :卵巢癌细胞株SKOV 3ipl在缺氧培养 8至 1 6小时内发生凋亡、细胞增殖抑制 ,2 4小时后对缺氧耐受 ,细胞增殖 ;
短句来源
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  hypoxia culture
     Methods: The ASODN and sense oligodeoxynucleotides (SODN) were designed on the basis of the HIF-1α cDNA sequence. The hypoxia culture model was established with a hypoxia incubator. The gene expression levels of HIF-1α and VEGF were observed under different hypoxia culture conditions using semi-quantitative reverse transcription PCR (RT-PCR).
     方法:设计针对HIF-1αRNA亚基模板序列的反义﹑正义寡核苷酸(senseoligodeoxynucleotides,SODN),并建立骨肉瘤细胞体外缺氧培养模型,观察缺氧培养不同时相HIF-1α反义寡核苷酸对骨肉瘤细胞系MG-63HIF-1α和VEGF的表达的影响。
短句来源
     Methods:The ASODN and sense oligodeoxynucleotides(SODN) were designed on the basis of the HIF-1α cDNA sequence. The hypoxia culture model was established by a hypoxia incubator. The gene productions of HIF-1α、P53 and Bcl-2 were observed at different hypoxia culture phase.
     方法:设计针对HIF-1αRNA亚基的反义、正义寡核苷酸(senseoligodeoxynucleoti-des,SODN),并建立骨肉瘤细胞体外缺氧培养模型,观察缺氧培养不同时相(8、16、24h)5μmol/L的HIF-1α反义寡核苷酸对骨肉瘤MG-63细胞HIF-1α、P53、Bcl-2表达及细胞凋亡的影响。
短句来源
     METHODS:ASODN and sense oligodeoxynucleotides (SODN) were designed on the basis of the HIF-1α cDNA sequence. The hypoxia culture model was established by a hypoxia incubator. The gene productions of HIF-1α and p53 were observed at different hypoxia culture phases.
     方法:设计针对HIF-1α亚基的mRNA反义和正义寡核苷酸(sense oligodeoxynucleotides,SODN),并建立骨肉瘤细胞体外缺氧培养模型,观察不同时相5μmol/L的HIF-1αASODN对骨肉瘤MG-63细胞HIF-1α和p53表达的影响。
短句来源
  “缺氧培养”译为未确定词的双语例句
     The apoptotic rates of myocardiums with SNAP were (3 2±0 7)%, (7 8±0 7)% and (10 9±1 0)% respsctively.
     心肌细胞缺氧培养16h、32h、4 8h后加入SNAP ,再复氧 6h ,流式细胞仪检测其凋亡率分别为 :3 2 %± 0 7% ,7 8%± 0 7%和 10 9%±1 0 %。
短句来源
     Apoptotis rates of cardiomyocytes measured by flow cytometer after hypoxia for 16,32 and 48 h were (2.9±0.5)%,(6.2±0.8)% and (26.6±3 0)% respectively.
     应用流式细胞仪定量检测 ,心肌细胞缺氧培养 16 ,32 ,4 8h后 ,其凋亡率分别为 :(2 .9± 0 .5 ) % ,(6 .2± 0 .8) %和 (2 6 .6± 3.0 ) % ;
短句来源
     Apoptotic rate was measured by TUNEL staining. Results The cardiomyocytes occurred opoptosis after hypoxia injury, apoptotic rate of which at 6, 12, 24 and 48h after hypoxia was (3 3±0 9)%, (8 3±1 8)%, (16 1±2 6)% and (19 4±2 3)% respectively.
     TUNEL法定量检测 ,心肌细胞缺氧培养 6、12、2 4、48h后 ,其凋亡率分别为 :( 3 3± 0 9) %、( 8 3± 1 8) %、( 16 1± 2 6) %和( 19 4± 2 3 ) %。
短句来源
     A study on the expression of DNA damage and repair related genes after hypoxia in PC12 cell
     PC12细胞缺氧培养后DNA损伤和修复相关基因表达的研究
短句来源
     Chelerythrine groups, hypoxia incubated 24 hours with PKC inhibitor (chelerythrine), A 0 nmol/L;
     均缺氧培养 2 4h ; PKC抑制剂chelerythrine(CT)组 ,A :加入CT 0nmol/L ;
短句来源
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  hypoxic culture
Isolated rat islets were studied in vitro under normoxic and hypoxic culture conditions and gene expression was determined with semi-quantitative multiplex RT-PCR.
      
Hypoxic culture promoted the increased synthesis of mineralized matrix by MC3T3-E1 cells.
      
Alkaline phosphatase activity was initially increased during hypoxic culture, but decreased during osteogenesis.
      
Osteocalcin production was also increased by hypoxic culture, but decreased after mineralization.
      
It prevents lipid peroxidation of glomerular mesangial cells and renal tubular epithelial cells exposed to high glucose or hypoxic culture conditions.
      
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  anaerobic culture
Collection and transport of specimens for anaerobic culture
      
A gassed-out tube or vial is the method of choice for transporting fluid specimens for anaerobic culture.
      
In order to assess the rapid laboratory diagnosis of anaerobic pyogenic infection, we compared the results of Gram stains, ultra-violet fluorescence and gas chromatography, all performed directly on pus, with those of anaerobic culture.
      
Each culture was serially transferred 10 times in anaerobic culture with sugar-limited medium containing xylose, but noselective antibiotic.
      
An average of 93 and 95% of the FBR4 and FBR5 cells, respectively, maintained pLO1297 in anaerobic culture.
      
更多          
  anaerobic incubation
The numbers of microscopic fungi isolated from soil samples after anaerobic incubation varied from tens to several hundreds of CFU per one gram of soil; a total of 30 species was found.
      
In selected line, about 50% of cells retained viability and could resume growth even after 96-hour-long anaerobic incubation.
      
Lipid bodies appeared only during 48-h anaerobic incubation of detached coleoptiles in the absence of exogenous glucose, when mitochondria degraded.
      
There was no change either in the double bond index of FAs, or in the qualitative and quantitative composition of FAs during shoot anaerobic incubation.
      
By contrast, Chlorella ellipsoidea (strain Marburg St), Hormidium flaccidum, and Stichococcus bacillaris do not develop hydrogenase activity during up to 100 hours of anaerobic incubation.
      
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  其他


This article presented an experimental reacarch of the effects of saponins extracted from panax ginseng fruit ( SPGF ) on cultured neonatal rat heart cells. The results shwoed that SPGF increased the synthsis of DNA in the cultured neonatal rat heart cells and protected the ischemic injured heart cells which were cultured under oxygen and glucose deprivation.

本文报告了人参果总皂甙对乳鼠原代培养心肌细胞作用的实验研究。实验结果表明:人参果总皂甙具有促进乳鼠培养心肌细胞DNA合成的作用;对缺糖缺氧培养的缺氧性损伤的心肌细胞具有保护作用。

Tetrahymena shanghaiensis Sl is an aerobic organism,but it can grow well forlong time in the medium with low O_2 concentration,even in absence of O_2.Thecontent of dissolved O_2 is very different in the medium of growing T.Sl underdifferent physiological conditions.We have studied LDH isoenzyme of T.Sl in different physiological conditions. It is obvious that cell in long-term maintenance medium contains higher level ofLDH_1,LDH_2,LDH_3,LDH_5.Cell in dead phase consists of LDH_1,LDH_2,LDH_3 at70 hr,but consists...

Tetrahymena shanghaiensis Sl is an aerobic organism,but it can grow well forlong time in the medium with low O_2 concentration,even in absence of O_2.Thecontent of dissolved O_2 is very different in the medium of growing T.Sl underdifferent physiological conditions.We have studied LDH isoenzyme of T.Sl in different physiological conditions. It is obvious that cell in long-term maintenance medium contains higher level ofLDH_1,LDH_2,LDH_3,LDH_5.Cell in dead phase consists of LDH_1,LDH_2,LDH_3 at70 hr,but consists of LDH_5 only at 90hr.Cell in log.phase contains higher levelof LDH_2,in addition to,LDH_1,LDH_5 visible on the gel.

原生动物四膜虫 Tetrahymena shanghaiensis Sl 是一种好氧性生物,但是在少氧甚至缺氧的培养液里能够存活而且存活相当长的时间。在不同的生理状态下生长的四膜虫,经测定其培养液中溶解氧的含量是不同的,在四膜虫生长的前对数期时,其培养液里溶解氧已有很大减少,长期保持和衰老期的四膜虫溶液中的溶解氧接近零或非常少;四膜虫乳酸的含量也有差别,长期保持溶液中的四膜虫细胞乳酸含量要比衰老期、对数期都大;四膜虫乳酸脱氢酶同工酶经聚丙烯酰胺凝胶电泳分析,表明在不同状态下该酶蛋白带有明显差别,主要反映在对数生长期细胞的蛋白带不同于衰老期、长期保持的细胞所具有的蛋白带。

Tetrahymena shanghaiensis Sl is an aerobic organism,but it can grow well for long time in the medium with low O_2 concentration,even in absence of O_2.The content of dissolved O_2 is very different in the medium of growing T.Sl under different physiological conditions. We have studied LDH isoenzyme of T.Sl in different physiological conditions. It is obvious that cell in long-term maintenance medium contains higher level of LDH_1,LDH_2,LDH_3,LDH_5.Cell in dead phase consists of LDH_1,LDH_2,LDH_3 at 70 hr,but...

Tetrahymena shanghaiensis Sl is an aerobic organism,but it can grow well for long time in the medium with low O_2 concentration,even in absence of O_2.The content of dissolved O_2 is very different in the medium of growing T.Sl under different physiological conditions. We have studied LDH isoenzyme of T.Sl in different physiological conditions. It is obvious that cell in long-term maintenance medium contains higher level of LDH_1,LDH_2,LDH_3,LDH_5.Cell in dead phase consists of LDH_1,LDH_2,LDH_3 at 70 hr,but consists of LDH_5 only at 90hr.Cell in log.phase contains higher level of LDH_2,in adition to,LDH_1,LDH_5 visible on the gel.

原生动物四膜虫 Tetrahymena shanghaiensis Sl 是一种好氧性生物,但是在少氧甚至缺氧的培养液里能够存活而且存活相当长的时间。在不同的生理状态下生长的四膜虫,经测定其培养液中溶解氧的含量是不同的,在四膜虫生长的前对数期时,其培养液里溶解氧已有很大减少,长期保持和衰老期的四膜虫溶液中的溶解氧接近零或非常少;四膜虫乳酸的含量也有差别,长期保持溶液中的四膜虫细胞乳酸含量要比衰老期、对数期都大;四膜虫乳酸脱氢酶同工酶经聚丙烯酰胺凝胶电泳分析,表明在不同状态下该酶蛋白带有明显差别,主要反映在对数生长期细胞的蛋白带不同于衰老期、长期保持的细胞所具有的蛋白带。

 
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