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   无氮培养基 的翻译结果: 查询用时:0.69秒
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无氮培养基
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  nitrogen-free medium
     A total of 29 isolates were selectively obtained from these rhizosphere soli samples based on their growth on nitrogen-free medium and their resistance to 100℃ for 10-15 min.
     首先利用芽孢杆菌中芽孢的抗热性将土壤溶液在100℃沸水中煮10-15分钟,然后用选择性无氮培养基进行初筛得到29株菌落形态不同的菌株;
短句来源
     It grew in HB 111 nitrogen-free medium and under light in-tensitly 130 μE/(m2·s). Exponential growth phase was between 4-8h in our batch culture.
     该藻能在水生111(HB111)无氮培养基中,在130μE/(m~2·s)下于4—8h内呈指数生长。
短句来源
     The quantifies of excreted ammonium by mutants were 7.5~14.0retool/L in nitrogen-free medium.
     在无氮培养基上,其氨分泌量可达7.5~14.0mmol/L。
短句来源
  “无氮培养基”译为未确定词的双语例句
     K19 1 could grow on N 2 free medium at 40℃ and reduce acetylene and 15 N 2 at 40℃.
     K19-1 在40℃,于无氮培养基上生长良好,并具有乙炔还原及15N2 还原能力。
短句来源
     The optimum pH for photosynthesis was 7.0–7.5 and that for respira-tion was 9.0. Photosynthetic rate of cells was increased about 20% when 30 mmol·L-1 NaNO3 was added into medium.
     发菜细胞光合作用的最适pH值为7.0 ̄7.5,呼吸作用最适pH值为9.0。 BG110无氮培养基中添加30mmol·L-1NaNO3,发菜细胞的光合速率增加约20%。
短句来源
     The results showed YUAN63 strain and strain YUAN100 could grow under pure N_2 and air condition,however,both could not grow under pure CO_2 and pure Ar2 condition.
     结果表明,纯N2条件下,菌株YUAN63和菌株YUAN100在无氮培养基上能生长,与空气条件下相当,但在CO2条件下未有可见菌落,在氩气条件下仅有少数小菌落。
短句来源
     1. The strain GY04 was screened by non-nitrogenous medium、 re-screening medium and basal medium from soil sample obtained in Guiyang.
     1.采用无氮培养基、复筛培养基及基本发酵培养基从贵阳市郊土壤中筛选得到一株野生菌株,编号为GY04。
短句来源
     TWO strains of nifogen fixing bacteria, Klebsiella pneumoniae K-12 and Enterobscter sp. E-25, isolated from the rhizosphere of local paddy fields were examined for their associative nitrogen fixation with rice seedlings cultured on C and N free media, in comparison with two known strains Azos-Pirillum lipoferum FS and Klebsiella oxytaca NG-13 introduced from abroad. All of the above strains could grow and possess associative nitrogen fixation activity under dark incubation conditions at 28? .
     从水稻根际分离的两株固氮菌:肺炎克雷从伯氏菌(Klebsilla Pneumoniae)K—12,肠杆菌属(En—terobacter sp)E—25以及从国外引进的水稻根际固氮螺菌(Azospirill-um lipoferum FS),克雷伯氏菌(Klebsiella oxytoca NG13)菌株,分别接种于有水稻幼苗生长的无碳、无氮培养基中,于28℃暗培养条件下进行培养.
短句来源
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  相似匹配句对
     Most sporangia and hypha were broken after storage in simple nitrogen-free liquid medium for 6 years.
     发现在液体培养基中保藏6年的Frankia.
短句来源
     (4) Protein free group.
     (4)饲料组。
短句来源
     It grows well in Ashby nitrogen-free culture medium, thus having broad prospects for use.
     该菌株在阿须贝培养基上生长良好,具有良好的应用前景。
短句来源
     The Frankia isolates grown in N - deficient BAP medium exhibited an acetylene reducing activity.
     这些分离株在BAP培养基上表现出乙炔还原活性。
     Studying on No Nitrogenous Furan Resin Used for Casting Steel
     铸钢呋喃树脂的研制
短句来源
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  nitrogen free medium
coli C strain harbouring the plasmid pWK120 grew on nitrogen free medium and reduced acetylene.
      
Light and scanning electron microscopy of it in nitrogen free medium revealed a filamentous mat of septate and branched hyphae bearing sporangia and vesicles capable of fixing nitrogen.
      
However, it is formed even during incubation of the washed cells in a nitrogen free medium.
      
  nitrogen-free medium
In plants grown on a nitrogen-free medium, the leaf accumulated lesser amounts of reduced nitrogen and higher amounts of starch, but the content of chloroplast protein corresponded to that of NO3- treatment.
      
Changes in the redox states of photosystem I (PSI) and PSII in irradiated wheat leaves were studied after growing seedlings on a nitrogen-free medium or media containing either nitrate or ammonium.
      
The alga develops the capacity to synthesize the enzyme when it is transferred from the ammonium medium to a nitrogen-free medium.
      
By contrast, whereas the synthesis of the enzyme in nitrogen-free medium proceeds at an increasing rate, in the nitrate medium it attains a stationary level after a short time.
      
Anabaena variabilis can be cloned in the dark from fragments with one and few cells, with an efficiency of about 40%, on the nitrogen-free medium of Allen and Arnon solidified with 0.5% agarose and supplemented with 5 mM fructose.
      
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The investigation deals,with the nitrogen fixation of some blue-green algae isolated from the soils of rice fields.Samples of algae-bearing soils were collected from rice fields in the provinces of Hupeh,Hunan and Kiangsi.Uni-algal cultures were made first on media enriched with nitrogenous compounds to encourage vigorous growth.The uni-algal cultures were then subcul- tured onto nitrogen-free media,which effected a preliminary separation of those algae which could thrive in the absence of pre-formed nitrogenous...

The investigation deals,with the nitrogen fixation of some blue-green algae isolated from the soils of rice fields.Samples of algae-bearing soils were collected from rice fields in the provinces of Hupeh,Hunan and Kiangsi.Uni-algal cultures were made first on media enriched with nitrogenous compounds to encourage vigorous growth.The uni-algal cultures were then subcul- tured onto nitrogen-free media,which effected a preliminary separation of those algae which could thrive in the absence of pre-formed nitrogenous compounds,from those which could not.The algae which showed a prolific growth on the nitrogen-free medium may be considered either to be capable of“fixing”(assimilating)atmospheric nitrogen,or that their nitrogen supply is coming from Azotobacter present in the culture.Our next step was to obtain bacteria-flee unialgal cultures. Two bacteriostatic methods were used:ultra-violet radiation from 300-watt quartz lamps,and treatment with streptomycin. Bacteriostatically treated uni-algal cultures were tested by inoculating sterile nitrogen-free media suitable for Azotobacter and allied organisms.Examination of these subcultures from the irradiated material showed that an intermittent radiation from our lamps of 5 minutes,repeated two or three times,was an effective bacteriostatic.Also,that Azotobacter and other micro-organisms were killed after treatment with 20 ppm.of streptomycin. The capacity for assimilating free nitrogen from the air was assessed by determining the quantity of nitrogenous compounds produced in the algal cells and in the medium,by means of the micro- Kjeldhal method.The nitrogen-fixing capacity has now been assessed on 4 isolated types of Blue- green Algae:HB 686(dnabaena azotica);HB 678(dnabaena azotica f.alpha);HB 670 (Anabaena variabilis forma);and HB 508 (Nostoc Linckia forma).The highest rate of fixation, amounting to 1.0146 rag.N/100 cc.of nitrogen-flee medium in 4 days,was attained by HB 686; the next,of 0.9382 mg.N,by HB 678,0.8614 nag.N,by HB 670;and 0.7592 mg.N,was produced under the same conditions,by HB 508.

湖北、湖南和江西等地采集的稻田蓝藻经过分离、培养、缺氮培养初步找到可能固氮的蓝藻后,进一步得出了无菌的纯培养的蓝藻藻种,经过试验和用微量凯氏法测定其产生的氮量,确定了四种蓝藻系固氮蓝藻。在100毫升无菌无氮培养基中生长四天的结果测定,水生686固氮蓝藻(Anabaena azotica)、水生678固氮蓝藻(A.azotica forma a)、水生670固氮蓝藻(Anabaena variabilis forma)和水生508固氮蓝藻(Nastoc Linckia forma)的固氮量分别为1.0146、0.938、0.8614和0.759毫克。

In our transformation experiments, the DNA was isolated chemically from cell of he-terocystous Anabaena 7120, and was used as donor. Oxygen sensitive nitrogen-fixing mutantof the Anabaena 7120——Anabaena-1 was used as recipient. In many tests, only two of themshowed positive results. The transformants were capable of growing in a medium without combined nitrogen sources under air condition. Their acetylene reduction activity was similar to that of the wild-type. The results showed that the oxygen-scavenging system...

In our transformation experiments, the DNA was isolated chemically from cell of he-terocystous Anabaena 7120, and was used as donor. Oxygen sensitive nitrogen-fixing mutantof the Anabaena 7120——Anabaena-1 was used as recipient. In many tests, only two of themshowed positive results. The transformants were capable of growing in a medium without combined nitrogen sources under air condition. Their acetylene reduction activity was similar to that of the wild-type. The results showed that the oxygen-scavenging system was recovered by transformation in the oxygen-sensitive mutant——Anabaena-1. The transformationfrequency is about 10-6-10-5. It is concluded that mutant of the Anabaena 7120 possess-es an ability for the uptake and integration of exogenous DNA. The difficulties of transformation in heterocystous filamentous Anabaena7120 were discussed. The higher extracellular deoxyribonuclease activity of the recipient may be one of the major factors.

用化学方法从固氮蓝藻鱼腥藻(Anabaena 7120)细胞中有效地提取了DNA,以此为供体DNA,用它的氧敏感固氮突变种鱼腥藻一1(Anabaena-l)为受体进行转化实验。在大量的转化实验中,仅有两次获得转化后的突变种在空气中、在无氮培养基上能生长,其转化频率为10~(-6)—10~(-5)。转化子在有氧条件下的乙炔还原活力相当于野生种。它表明突变种的除氧系统通过转化而得到恢复。推测鱼腥藻7120突变种可能具有吸收和整合外源DNA的能力。对丝状蓝藻转化困难的原因进行了探讨,结果表明受体藻胞外DNA酶活力水平高于其他单细胞蓝藻,这可能是影响有效地转化作用的重要原因之一。

TWO strains of nifogen fixing bacteria, Klebsiella pneumoniae K-12 and Enterobscter sp. E-25, isolated from the rhizosphere of local paddy fields were examined for their associative nitrogen fixation with rice seedlings cultured on C and N free media, in comparison with two known strains Azos-Pirillum lipoferum FS and Klebsiella oxytaca NG-13 introduced from abroad. All of the above strains could grow and possess associative nitrogen fixation activity under dark incubation conditions at 28?. The nitrogen fixing...

TWO strains of nifogen fixing bacteria, Klebsiella pneumoniae K-12 and Enterobscter sp. E-25, isolated from the rhizosphere of local paddy fields were examined for their associative nitrogen fixation with rice seedlings cultured on C and N free media, in comparison with two known strains Azos-Pirillum lipoferum FS and Klebsiella oxytaca NG-13 introduced from abroad. All of the above strains could grow and possess associative nitrogen fixation activity under dark incubation conditions at 28?. The nitrogen fixing activities of these associations were as follows. 10.5 nmol C2H4/seedling/hr. for Azospirillum Hpoferum FS,4.9 nmol C2H4/seedling/hr.for Klebsiella pneumon-iae K-12, 3.9 nmol C2H4/seedling/hr. for Klebsiella oxytoca NG-13, and 2.9 nmol C2H4/seedling/hr. for Enterobacter sp. E-25 respectively. With the exception of the aerobic Azospirillvm lipofervm FS, the other 3 strains grew vigerously around the endosperm of the seedling and formed a bacteria ring. By using DNA specific flourescenCe dye DAPI staining and scanning electron microscopic observations revealed that these 4 strains of the nitrogen fixing bacteria adhered to the root surface and built up a dense Population, Particularly at the root hair region. Scanning electron microscopic observation of the transverse section of the root and the re-isolation experiments of the endophytes indicated that the bacteria could invade the Parenchyma of the root cortex and propagate within the cell.

从水稻根际分离的两株固氮菌:肺炎克雷从伯氏菌(Klebsilla Pneumoniae)K—12,肠杆菌属(En—terobacter sp)E—25以及从国外引进的水稻根际固氮螺菌(Azospirill-um lipoferum FS),克雷伯氏菌(Klebsiella oxytoca NG13)菌株,分别接种于有水稻幼苗生长的无碳、无氮培养基中,于28℃暗培养条件下进行培养.以上各菌均能正常生长,并与水稻幼苗有联合固氮作用.其固氮活性分别如下:Azospirillum lipoferum FS菌株为10.5毫微克分子C_2H_4/株/小时,Klebsiella oxytoca NG13菌株为3.9毫微克分子,C_2H_4/株/小时,Klebsiella pneumoniae K—12菌株为4.9毫微克分子C_2H_4/株/小时,Enterobacter SP E—25菌株为2.9毫微克分子C_2H_4/株/小时.固氮菌与水稻联合共生于半固体培养基中时,菌在水稻胚乳周围生长旺盛、繁殖快、并形成菌环.用对DNA专一的荧光染料DAPI染色观察,以及扫描电镜观察均表明:这四种固氮菌均聚集并附着在水稻根...

从水稻根际分离的两株固氮菌:肺炎克雷从伯氏菌(Klebsilla Pneumoniae)K—12,肠杆菌属(En—terobacter sp)E—25以及从国外引进的水稻根际固氮螺菌(Azospirill-um lipoferum FS),克雷伯氏菌(Klebsiella oxytoca NG13)菌株,分别接种于有水稻幼苗生长的无碳、无氮培养基中,于28℃暗培养条件下进行培养.以上各菌均能正常生长,并与水稻幼苗有联合固氮作用.其固氮活性分别如下:Azospirillum lipoferum FS菌株为10.5毫微克分子C_2H_4/株/小时,Klebsiella oxytoca NG13菌株为3.9毫微克分子,C_2H_4/株/小时,Klebsiella pneumoniae K—12菌株为4.9毫微克分子C_2H_4/株/小时,Enterobacter SP E—25菌株为2.9毫微克分子C_2H_4/株/小时.固氮菌与水稻联合共生于半固体培养基中时,菌在水稻胚乳周围生长旺盛、繁殖快、并形成菌环.用对DNA专一的荧光染料DAPI染色观察,以及扫描电镜观察均表明:这四种固氮菌均聚集并附着在水稻根表,大部分在根毛区.水稻根横切面的扫描电镜观察,以及对接种的水稻根内生菌的重新分离实验表明,部分固氮菌能够进入水稻根组织的皮层薄壁细胞内生长并繁殖.

 
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