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重组缺陷
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  “重组缺陷”译为未确定词的双语例句
     Objective To investigate the effect of recombinant nonreplicating adenovirus bearing the IL-10 gene (AdIL-10) on the expression of α-SMA, TGF-β1 and TNF-α in primary culture rat hepatic stellate cell (HSC) in vitro.
     目的应用携带大鼠白介素-10(IL-10)基因的重组缺陷型腺病毒(AdIL1-0)感染原代培养的大鼠肝星状细胞(HSC),通过体外实验观察外源性大鼠IL-10基因对HSC中αSMA、TGF-β1和TNFα表达的影响。
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     Conclusion The successful construction of ad5/CMV-TGF-β1 production in our experiment may lay foundation for the orthopaedic gene therapy such as in the treatment of the defect of bone, muscle tissue.
     结论重组缺陷型腺病毒载体Ad5/CMV-TGF-β1的构建成功,为下一步进行基因疗法治疗骨骼、肌肉组织缺损提供基础。
短句来源
     The assay systern empoloyed were Sal-monella/microsome test , using the five standard tester strains TA1535, TA1537,TA1538, TA98, TA100, and the rerair test with bacillus subtilis, using strainsdeficient in recombination repair M45 (Rec ) & wild bacteria H17 (Rec~+).
     对沙门氏菌/微粒体酶试验采用TA1535、TA1537、TA1533、TA98和TA100五种标准菌株; 对枯草杆菌重组缺陷型修复试验采用重组缺陷型枯草杆菌M45(Rec~-)和野生型H17(Rec~+)。
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     Methods A recombinant replication defective adenovirus, Adp27 KIP1 , was constructed and used to infect EJ cells. Seventy two hours after infection, the expression of p27 KIP1 protein in EJ was tested and the biological behavior of EJ was evaluated by growth curves, cloning efficiency and cell cycle distribution.
     方法 构建含人p27KIP1 基因的重组缺陷腺病毒,并感染EJ细胞,72 小时后检测p27KIP1 蛋白的表达、生长曲线、克隆形成率和细胞周期分布。
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     Objective To construct recombinant adenoviral type 5 vector containing both cytomegalovirus promotor and transforming growth factor-β1. Methods The two plasmid pACCMV TGF-β1 and pJM17 were transferred into the 293 cells (human transformed embryonic kidney cells) and then, the recombinant adenovirus was constructed by homologous recombination.
     目的构建重组缺陷型腺病毒载体Ad5/CMV-TGF-β1。 方法利用同源重组的原理,将含有TGF-β1基因cDNA的pACCMVTGF-β1质粒和pJM17质粒在腺病毒包装细胞中共转染,制备重组复制缺陷腺病毒Ad5/CMV-TGF-β1并进行鉴定。
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     The recombinant plasmid expressed well in E.
     从重组E.
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     Imperfection and improvement for the recoganization rules of new debts
     新债务重组准则缺陷及改进
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     Identification of Homologous Recombination of Lincomycin Biosynthesis Defective Strains
     林可霉素生物合成缺陷的同源重组菌鉴定
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     Defects of Corrugated Box
     纸箱的缺陷
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     Rebuild CRM
     重组CRM
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Mutagenic effect of the dusts collected from a tin mine in Yunnan Provincewas identified with bacterial mutation assay.The assay systern empoloyed were Sal-monella/microsome test , using the five standard tester strains TA1535, TA1537,TA1538, TA98, TA100, and the rerair test with bacillus subtilis, using strainsdeficient in recombination repair M45 (Rec ) & wild bacteria H17 (Rec~+). Re-sults showed: the oxide mine, oxide mine of arsenical high quantity, sulfurmine, lead mine, granite mine and marble mine are...

Mutagenic effect of the dusts collected from a tin mine in Yunnan Provincewas identified with bacterial mutation assay.The assay systern empoloyed were Sal-monella/microsome test , using the five standard tester strains TA1535, TA1537,TA1538, TA98, TA100, and the rerair test with bacillus subtilis, using strainsdeficient in recombination repair M45 (Rec ) & wild bacteria H17 (Rec~+). Re-sults showed: the oxide mine, oxide mine of arsenical high quantity, sulfurmine, lead mine, granite mine and marble mine are not mutagenic in this assay.The authors have discused the results.

通过微生物突变试验,检测云锡矿尘的致突变性。对沙门氏菌/微粒体酶试验采用TA1535、TA1537、TA1533、TA98和TA100五种标准菌株;对枯草杆菌重组缺陷型修复试验采用重组缺陷型枯草杆菌M45(Rec~-)和野生型H17(Rec~+)。实验结果提示:氧化矿、铅化矿、硫化矿、花岗岩矿、大理岩矿和富砷氧化矿六种矿尘均无致突变性。并对实验结果进行了初步探讨。

Natural competence-deficient mutant of Bacillus subtilis BR151, which carries plasmid pUB110, was obtained by nitrosoguanidine mutagenesis on solid medium From 2949 clones, a mutant, the transformation frequency of which is 10 2 ~10 3 times lower than that of the control, was obtained and tested for the changes of trophic type, resistance to Km, plasmid and sensitivity to UV The results indicate the reduction of transformation frequency is due to competence-deficient mutation

以携带pUB110质粒的枯草芽孢杆菌BR151菌株为出发菌株,利用亚硝基胍作诱变剂,直接在LB平板上进行诱变。从2949个菌落中筛选出一个转化频率低于出发菌株2~3个数量级的突变株,并对其营养缺陷型、UV的敏感性及Km抗性和质粒进行了检测,确证其转化能力降低为自然感受态缺陷而非营养缺陷型的改变或重组缺陷所致

Objective To investigate the influence of over expression of p27 KIP1 on the malignant phenotype of EJ human bladder carcinoma cell line. Methods A recombinant replication defective adenovirus, Adp27 KIP1 , was constructed and used to infect EJ cells. Seventy two hours after infection, the expression of p27 KIP1 protein in EJ was tested and the biological behavior of EJ was evaluated by growth curves, cloning efficiency and cell cycle distribution. Results Over expression of p27 KIP1...

Objective To investigate the influence of over expression of p27 KIP1 on the malignant phenotype of EJ human bladder carcinoma cell line. Methods A recombinant replication defective adenovirus, Adp27 KIP1 , was constructed and used to infect EJ cells. Seventy two hours after infection, the expression of p27 KIP1 protein in EJ was tested and the biological behavior of EJ was evaluated by growth curves, cloning efficiency and cell cycle distribution. Results Over expression of p27 KIP1 protein was observed in Adp27 KIP1 infected cells. As a result, the proliferation of the cells was greatly inhibited, the cells being arrested in G1 phase. Conclusions The over expression of p27 KIP1 may inhibit the proliferation of EJ cells, suggesting that p27 KIP1 play a pivotal role in the genesis of bladder carcinoma and that Adp27 KIP1 might be used as a potential therapeutic method for the disease.

目的 探讨p27KIP1 的过度表达对人膀胱癌细胞系EJ的影响。 方法 构建含人p27KIP1 基因的重组缺陷腺病毒,并感染EJ细胞,72 小时后检测p27KIP1 蛋白的表达、生长曲线、克隆形成率和细胞周期分布。 结果 转基因后,EJ细胞过度表达p27KIP1 蛋白,增殖明显受阻,细胞停滞于G1 期。 结论 p27KIP1 的过度表达可明显抑制EJ细胞的增殖,提示p27KIP1 基因在人膀胱癌的发生中起重要作用,用重组腺病毒转p27KIP1 基因可成为潜在的膀胱癌治疗方法。

 
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