助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   重组缺失 的翻译结果: 查询用时:0.201秒
图标索引 在分类学科中查询
所有学科
更多类别查询

图标索引 历史查询
 

重组缺失
相关语句
  “重组缺失”译为未确定词的双语例句
     It is in novel azoospermic patients with b2/b4 recombinogenic deletion and the frequency is 4.2%.
     b2/b4重组缺失的个体都为原发性无精症患者,缺失率为4.2%。
短句来源
     Normozoospermic group versus novel spermatogenic impairment group,the P values of diversity SY1291-gr/gr-DAZ1/DAZ2,SY1191-b2/b3-DAZ3/DAZ4 and b2/b4deletion rate are 0.001,0.004 and 0.009,there are significant differences.
     SY1291-gr/gr-DAZ1/DAZ2、SY1191-b2/b3-DAZ3/DAZ4和b2/b4重组缺失在正常生精组与生精障碍组间缺失率差异的P值分别为0.001、0.004和0.009和,差异均有统计学意义。
短句来源
     SY1291-gr/gr-DAZ3/DAZ4 deletion may be affect slightly men spermatogenesis and exist as a kind of genomic polymorphism. SY1291-gr/gr-DAZ1/DAZ2 and SY1191-b2/b3-DAZ3/DAZ4 deletion are the high risk factor to male infertility,The deletion of b2/b4 is the cause which would result men's novel spermatogenic failure.
     SY1291-gr/gr-DAZ3/DAZ4缺失可能对生精功能的影响较小,仅是一种基因组多态,SY1291-gr/gr-DAZ1/DAZ2和SY1191-b2/b3-DAZ3/DAZ4缺失是男性生精障碍的高风险因子,b2/b4重组缺失是男性生精障碍的病因。
短句来源
     pHZ1252 was transformed into \%S. avermitilis\% and expressed VHb which had biological activity after thiostrepton induction. pHZ1252 was structurally unstable in \%S.
     pHZ1252 在阿维链霉菌中发生了重组缺失,但缺失的pHZ1252 上仍含有完整的vhb 基因及诱导型强启动子,且可在阿维链霉菌中稳定遗传,却不能再转化大肠杆菌。
短句来源
     coli\|Streptomyces shuttle vector for expressing VHb,in which vhb structural gene is controlled by thiostrepton\|inducible promoter P tipA . pHZ1252 was unstable in S.cinnamonensis ,occurring deletion recombination. The deletion DNA fragment of pHZ1252 included not only part of E.
     大肠杆菌 链霉菌穿梭表达载体pHZ12 5 2中的透明颤菌血红蛋白基因 (vhb)位于硫链丝菌素诱导启动子PtipA之下 ,它在肉桂地链霉菌中的结构不稳定 ,发生了重组缺失 ,缺失的片段包括大肠杆菌质粒部分和vhb基因。
短句来源
  相似匹配句对
     The recombinant plasmid expressed well in E.
     从重组E.
短句来源
     Autocatalytic Reconstitution of Deletion Mutants of Phycobiliprotein ApcE
     藻胆蛋白ApcE缺失突变体的自催化重组研究
短句来源
     The culture conditions of recombinant E.
     对重组E.
短句来源
     Construction of recombinant eukaryotic expression plasmid containing hepatitis B virus genome with partial deletion of the core promoter
     HBV核心启动子部分缺失重组质粒的构建
短句来源
     Malposition and Shortage
     错位与缺失
短句来源
查询“重组缺失”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
没有找到相关例句


There is a 13bp large reverse repeat sequence preceeded by a 7bp small repeat in the 3'flanking region of rbcL transcription unit of Nicotiana tabacum.A 383bp Xbal fragment containing these tandemly repeats was inserted into the plasmid pλSΔ.And then these two repeats and sequence downstream the repeats were separated and deleted systematically to obtain various deletions.The deletion pRTX65、pRTX74 and pRTX 83 have been sequenced to determine the deleted sequence precisely. S1 mapping has been adopted to investigate...

There is a 13bp large reverse repeat sequence preceeded by a 7bp small repeat in the 3'flanking region of rbcL transcription unit of Nicotiana tabacum.A 383bp Xbal fragment containing these tandemly repeats was inserted into the plasmid pλSΔ.And then these two repeats and sequence downstream the repeats were separated and deleted systematically to obtain various deletions.The deletion pRTX65、pRTX74 and pRTX 83 have been sequenced to determine the deleted sequence precisely. S1 mapping has been adopted to investigate the transcripts of these deletions. Results show us"that the stability of 306bp mature mRNA relies on the 3'large repeat and a short AU-rich sequence downstream the repeat.The accumulation efficiency of mature mRNA is estimated about 40% of the total readthrough mRNA in E.coli system. The regulation level of rbcL gene expression has also been investigated.The Xbal- EcoR1 fragment of pRTX65、pRTX74 and pRTX83 were transferred into pSP-TT~* in a position between spinach promoter and threonine(Thr)terminator.The results came from S1 mapping show us that the E.coli RNA polyermase reads through the rbcL 3'flanking region and stops at the stop site of Thr terminator.The 306bp mature rbeL mRNA can not be accumulated any more.It indicates that the rbcL gene expression might be controlled at the posttranscriptional level and mature rbcL mRNA might be the product of a precursor mRNA by a processing procedure.

烟草(Nicotianatabacum)rbcL 转录单位的3′末端存在着前后相连的小逆向重复顺序和大逆向重复顺序。我们对这两个重复顺序进行了系统性地删减并将这两个顺序单独分开,获得了一系列重组的缺失子。对其中的三个缺失子 pRTX65、pRTX74及 pRTX83作了 DNA 顺序分析,以精确地确定删去的碱基顺序。用 S1定位的分析方法考察了这些缺失子的转录产物。结果表明长度为306bp 的成熟 mRNA 的积累依赖于3′末端的大逆向重复顺序及转录单位下游的一小段 AU 丰富的顺序。在大肠杆菌中,这种成熟 mRNA 的积累效率为整个转录产物的40%左右。为了考察 rbcL 在3′末端的调控水平,我们将带有逆向重复顺序的片断插入质粒 pSP-TT~*的菠菜 rbcL 启动子及苏氨酸终止子之间。S1定位的结果表明,大肠杆菌的 RNA 多聚酶读过 rbcL 3′端的逆向重复顺序而全部停留在苏氨酸终止子的终止位点。这说明成熟的 mRNA 不是 rbcL 基因转录的直接产物,而是由前体mRNA 经转录后的加工作用产生的。3′末端的 AU 丰富顺序及大发夹结构可能是精确地进行转录后加工作用的某种信号。

Two expression vectors, pWY101 and pWY102, were constructed by cloning \%Vitreoscilla\% hemoglobin gene( \%vhb\%) with its native oxygen\|regulated promoter into \%E.coli\|Streptomyces\% shuttle vector pIJ653. They were introduced into \%Streptomyces avermitilis,\% but Western blotting experiment failed to detect \%vhb\% gene expression. pHZ1252 is another shuttle vector for expressing VHb, in which \%vhb\% structural gene is controlled by a strong, thiostrepton\|inducible \%Streptomyces\% promoter P\-\{\%tipA\%\}....

Two expression vectors, pWY101 and pWY102, were constructed by cloning \%Vitreoscilla\% hemoglobin gene( \%vhb\%) with its native oxygen\|regulated promoter into \%E.coli\|Streptomyces\% shuttle vector pIJ653. They were introduced into \%Streptomyces avermitilis,\% but Western blotting experiment failed to detect \%vhb\% gene expression. pHZ1252 is another shuttle vector for expressing VHb, in which \%vhb\% structural gene is controlled by a strong, thiostrepton\|inducible \%Streptomyces\% promoter P\-\{\%tipA\%\}. pHZ1252 was transformed into \%S.avermitilis\% and expressed VHb which had biological activity after thiostrepton induction. pHZ1252 was structurally unstable in \%S.avermitilis\%, occurring deletion recombination. However, the rest part of pHZ1252 was stable in \%S.avermitilis\% and still contained \%vhb\% gene and P\-\{\%tipA\%\}. Plasmid pHZ1252 isolated from \%S.avermitilis\% was unable to transform \%E.coli,\% showing the loss of part of \%E.coli\% plasmid from pHZ1252.

将含有自身启动子的透明颤菌血红蛋白基因( vhb) 克隆至大肠杆菌—链霉菌穿梭质粒载体pIJ653 中构建成表达载体p WY101 和p WY102 ,用它们转化阿维菌素(avermectins) 产生菌———阿维链霉菌( Streptomyces avermitilis) ,经Western blotting 分析并未检测到vhb 基因表达,但用穿梭载体pHZ1252( 其中的vhb 基因位于受硫链丝菌素诱导的链霉菌强启动子PtipA之下) 转化阿维链霉菌并经硫链丝菌素诱导,则在该菌中表达出了有活性的VHb 蛋白。pHZ1252 在阿维链霉菌中发生了重组缺失,但缺失的pHZ1252 上仍含有完整的vhb 基因及诱导型强启动子,且可在阿维链霉菌中稳定遗传,却不能再转化大肠杆菌。

Streptomyces cinnamonensis A 10 is a monensin\|producing strain. pHZ1252 is an E.coli\|Streptomyces shuttle vector for expressing VHb,in which vhb structural gene is controlled by thiostrepton\|inducible promoter P tipA . pHZ1252 was unstable in S.cinnamonensis ,occurring deletion recombination. The deletion DNA fragment of pHZ1252 included not only part of E.coli plasmid but also vhb gene. However,plasmid pHZ1252 isolated from S.avermitilis transformants,which lost part of...

Streptomyces cinnamonensis A 10 is a monensin\|producing strain. pHZ1252 is an E.coli\|Streptomyces shuttle vector for expressing VHb,in which vhb structural gene is controlled by thiostrepton\|inducible promoter P tipA . pHZ1252 was unstable in S.cinnamonensis ,occurring deletion recombination. The deletion DNA fragment of pHZ1252 included not only part of E.coli plasmid but also vhb gene. However,plasmid pHZ1252 isolated from S.avermitilis transformants,which lost part of E.coli plasmid but still contained vhb gene and P tipA was stable in S.cinnamonensis and expressed VHb with biological activity after thiostrepton induction. Shake flask fermentation experiments showed that the presence of VHb in S.cinnamonensis efficiently enhanced cell growth and improved antibiotic production under aeration\|poor conditions.

肉桂地链霉菌 (S .cinnamonensis)是莫能菌素 (Monensin)的产生菌。大肠杆菌 链霉菌穿梭表达载体pHZ12 5 2中的透明颤菌血红蛋白基因 (vhb)位于硫链丝菌素诱导启动子PtipA之下 ,它在肉桂地链霉菌中的结构不稳定 ,发生了重组缺失 ,缺失的片段包括大肠杆菌质粒部分和vhb基因。但来自阿维链霉菌 (S .avermitilis)中缺失了大肠杆菌质粒部分却保留了完整的vhb基因及tipA启动子的 pHZ12 5 2 ,可在肉桂地链霉菌中稳定复制 ,不再发生缺失 ,经硫链丝菌素诱导表达出了有生物活性的VHb蛋白。摇瓶发酵实验证明 ,VHb蛋白在氧限条件下可明显促进肉桂地链霉菌的菌体生长和抗生素合成。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关重组缺失的内容
在知识搜索中查有关重组缺失的内容
在数字搜索中查有关重组缺失的内容
在概念知识元中查有关重组缺失的内容
在学术趋势中查有关重组缺失的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社