震荡培养 shake culture(1)   shake culture      The callus could differentiate into the plantlets on solid culture and into plenty of tubers on liquid shake culture on MS mediums without plant growth hormone.      愈伤组织在不含激素的MS固体培养基上可一步分化成完整小植株，在液体培养基中震荡培养可形成大量小块茎。 短句来源   “震荡培养”译为未确定词的双语例句      That were FeSO_4 0.02g/L, MgS0_40.04g/L, initial pH7.0-8.0, inoculation quantity 4% with 50 mL medium in 250mL flask, 180 r/min, 30℃, and culture time 72 h.      其他发酵条件为：微量元素为FeSO_40.02g／L、MgSO_40.04g／L，初始pH7.0-8.0，装液量为50mL／250mL，接种量为4％，30℃，180rpm震荡培养72h。 短句来源      The engineered strain E. Coli DH5 α/pRL-B1 was cultured in LB medium(containing100μg/mL Amp) at 37℃, 180rpm, 16 hours with 5% inoculation.      本研究将重组质粒pRL-B1 转入大肠杆菌DH5α,以5%的接种量转接到LB 液体培养基(含100μg/mL 氨苄青霉素)中,37℃,180rpm 震荡培养13h。 短句来源      The recombination expression vector, pPIC9K-E~(rns), was linearized by Sal 1 and electroporated into P.PastorisGS115. The recombinant plasmid GS115-pPIC9K- E~(rns) was obtained by selected.      重组表达载体pPIC9K-E~(rns)经Sal Ⅰ线形化后，电转化整合到毕赤酵母GS115基因组上，阳性转化子命名为GS115-pPIC9K-E~(rns)。 将GS115-pPIC9K-E~(rns)在30℃条件下震荡培养，以1％的甲醇诱导，BVDV E~(rns)融合蛋白在毕赤酵母表达系统中获得表达。 短句来源      L-1 2,4-D and 1. 0 mg . L-1 KT. Callus was induced and then transferred into MS li-oid medium without 2,4-D and KT.      无菌苗下胚轴为外植体,在附加0.5mg·L~(-1)2,4-D和1.0mg·L~(-1)KT的MS固体培养基上诱导出胚性愈伤组织后,转入到去掉2,4-D和KT的MS液体培养基中经悬浮震荡培养产生了胚状体,在分化培养基中诱导生根。 短句来源      Incubation of Trix-Ⅲa for expression was studied. The optimized conditions for induction of Trix-Ⅲ a was: the concentration of the BL21 Ⅲa before induction amounted to OD6000.4-0.6.,37℃,0.2 mM IPTG, incubating 2-3hrs after incubation.      通过摸索不同诱导条件、不同诱导所需IPTG浓度，优化了Ⅲa蛋白的表达条件：30℃培养待菌生长至OD600为0.4-0.6时，用终浓度为0.2mM的IPTG诱导，37℃震荡培养4-5h。 短句来源 更多          相似匹配句对      On the Cultivation of Esthetic Judgment      审美能力的培养 短句来源      cultivated for 144 h.      培养144 h. 短句来源      Vibrations on the View of Life Ethics      生命伦理观的震荡 短句来源      The shock of high oil prices      高油价震荡 短句来源      Methylene Oxide in Beer Made a Great Impact on People      啤酒“甲醛”震荡波 短句来源 查询“震荡培养”译词为用户自定义的双语例句     我想查看译文中含有：的双语例句 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法，我们为您准备了出自英文原文的大量英语例句，供您参考。   shake culture Small pieces of this material were cut off, cleaned, and used as inocula for shake culture experiments. 例句来源       Cell yields of 1.4x107 cells/ml were obtained in this medium in aerobic shake culture. 例句来源       The auxanographic deep agar shake culture method was used; the gas phase contained 5% O2 and 1% CO2 in N2. 例句来源       The influence of inorganic phosphate and immobilization on cells of Claviceps purpurea strain 1029/N5 producing ergot peptides in shake culture was examined. 例句来源       The optimized culture medium and conditions (33° C) gave 0.55 unit of FPase, 188.1 units of xylanase and 3.37 units of β-glucosidase per milliliter of culture filtrate at 144 h of shake culture. 例句来源       更多                    Embryoids were obtained from the pollen callus of grape. Afther they hald developed into young halploid plantlets on the solid culture medium, some epidermal cells of the mesocotyl.hypcotyl, leaf and the apper part of the root dedifferentiated to embryonic state end devided actively From one or two suck cells amulticellulur mass was formed on the surface and then developed into an embryoid which always grew toa plantlet, when slaughed off and cultured.             由葡萄(Vitis Vinisera L)花药愈伤组织产生的胚状体形成单倍体幼苗后,经4—5次继代培养,发现其表皮细胞具有形成胚状体和进一步发育成幼苗的能力。胚状体的表层也具有同样的能力。将这样的一棵幼苗,整株置于液体培养中,在黑暗中震荡培养一周,然后在光照下静止培养,可获得大量完整的小植栋。实验结果证明葡萄单倍体的体细胞具有再生完整植株的全能性。在植物组织和细胞培养中,通过胚状体再进一步形成幼植物具有繁殖数量多、成苗速度快和结构完整的特点。因此,对于胚状体发生的研究,已经引起人们广泛的注意。目前,在植物的组织培养中,记载有胚状体发生的植物已涉及到广泛的分类类群,产生胚状体的离体培养物也有多种多样。但是对于胚状体形态发生的详细研究报导甚少。在1965年,Konor和Nataraja利用石龙芮的下胚轴进行切片观察时,看到了一系列胚状体发生的组织学过程。1970年Backs-Hüseman,D等在胡萝卜的单细胞悬浮培养中,见到了游离单细胞发育成胚状体的过程,1974年,Sunderland等在烟草中,也报导了小孢子胚状... 文摘来源          Hypocotyl segments of aseptic seedlings of sesame were cultured on MS solid medium supplemented with 0. 5 mg. L-1 2,4-D and 1. 0 mg . L-1 KT. Callus was induced and then transferred into MS li-oid medium without 2,4-D and KT. Embryoids were induced by suspension culture. Embryogenesis was observed in the parenchyma around vascular tissue.During the embryoid development, the total content of ZR + ZT and that of ABA were 10 to 50 times higher than that of IAA, and the content of ZR + ZT was negatively correla...             以芝麻（Sesamicm indicum L.）无菌苗下胚轴为外植体,在附加0.5mg·L~(-1)2,4-D和1.0mg·L~(-1)KT的MS固体培养基上诱导出胚性愈伤组织后,转入到去掉2,4-D和KT的MS液体培养基中经悬浮震荡培养产生了胚状体,在分化培养基中诱导生根。胚状体发生于胚轴维管组织周围薄壁细胞处。在体细胞胚胎发育过程中,ZR+ZT与ABA总量比IAA高10～50倍,ZR+ZT的含量变化与ABA呈负相关,非胚性愈伤组织向胚性愈伤组织转化时IAA和ABA含量升高而ZR+ZT则相反。不同组织可溶性蛋白质分子量分别为:白色愈伤组织110kD,绿色愈伤为27和84kD,胚性愈伤组织为27和50kD,球形胚和心形胚为15、27、84kD,子叶形胚为48kD,根为55kD。 文摘来源          Callus were induced from explants of bulbils of Pinellia ternata Breit on MS medium with IAA 3.0 mg/L +BA0.5mg/L The milky callus induced from bulbils grown quickly. The callus could differentiate into the plantlets on solid culture and into plenty of tubers on liquid shake culture on MS mediums without plant growth hormone. The survival rate of transplanted plantlet was 92% The germination capacity of transplanted tubers was 54.3%             用半夏珠芽作外植体，在添加ＩＡＡ３．０ｍｇ／Ｌ和ＢＡ０．５ｍｇ／Ｌ的ＭＳ固体培养基上可诱导出一种乳白色生长快的愈伤组织。愈伤组织在不含激素的ＭＳ固体培养基上可一步分化成完整小植株，在液体培养基中震荡培养可形成大量小块茎。小植株移栽成活率９２％，小块茎种植萌发成苗率５４．３％。 文摘来源   << 更多相关文摘         相关查询: 培养 震荡 缺氧培养 培养烧瓶 露天培养 好气培养 存贮培养 试管培养 血液培养 供气培养 培养酵母 培养罐  对查询结果不满意

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