To study drug resistance mechanism of gastrointestinal tumor,doxorubicin resistant cell line of human gastric cancer,we developed BGC 823/DOX, by increasing doses of doxorubicin. We completed chromosomal analysis, double time of cell grow, cell cycle distributions,drug resistance and cross resistance, and studied reversing drug resistance.
METHODS The transmission and scanning electron microscopy and flow cytometry were used to observe the changes of cells ultra-structure and cell cycle distributions. The drug resistance, cross-resistance and reversing drug resistance were studied by MTT.
Salvicine (SAL) was used to reverse the drug resistance in a gemcitabine-resistant pancreatic cancer cell line (SW1990-GEM). RT-PCR, flow cytometry and MTT assay were employed to evaluate the effect of reversing drug resistance by salvicine.
Conclusion Mechanism of Chinese drug MYJ-G reversing multidrug resistance could be that it inhibits "drug pump" function of P-gp so as to make intracellular drug accumulation increased, but it does not affect mdr1 gene and P-gp expression level.
Study of the Effects of Carnosic Acid,Garlic Oil on Differentiation and Apoptosis of Leukemia Cells and Carnosic Acid on Reversing Resistance to Retinoic Acid
AIM: To observe the inhibitory effect of dihydroartemisinin and cisplatin on human lung adenocarcinoma cell line A549 and A549/CDDP, and determine the effect of resistance reversion of dihydroartemisinin in vitro.
Objective To observe the inhibiting effect of dihydroartemisinin and cisplatin on human lung adenocarcinoma cell line A549 and A549/CDDP ,and determine the effect of resistance reversion of dihydroartemisinin in vitro.
Objective: To observe the effect of dihydroartemisinin and cisplatin on human lung adenocarcinoma cell line A549/CDDP,and determine the effect of resistance reversion of dihydroartemisinin in vitro. To analyse the effects of dihydroartemisinin on apoptosis of the human lung adenocarcinoma cell line A549/CDDP in vitro.
Our results suggested that deguelin was a novel anti-leukemia agents with high efficacy and low toxicity and it is also a promising agent for reversing drug resistance.
Therefore, pharmacological inhibition of DNA damage repair pathways has been explored as a useful strategy to enhance chemo and radiosensitivity, thus it could be used for reversing drug resistance.
Therefore, pharmacological inhibition of DNA damage repair pathways has been explored as a useful strategy to enhance chemo and radiosensitivity, thus it could be used for reversing drug resistance.
Experiments showed that CHM played its anticancer role by inducing apoptosis and differentiation, enhancing the immune system, inhibiting angiogenesis, reversing multidrug resistance (MDR), etc.
These data illustrate that B859-35, a compound with antitumor activity in several tumors, is at least ten times more potent than racemic verapamil in reversing multidrug resistance.