To find a protocol of extracting RNA that has high production and good quantity from both wheat leaves and rust,Trizol,Licl,SDS and Bizol protocols were used for extracting total RNA from rust induced wheat leaves,total RNA extracted by Trizol and Bizol protocols was amplified by long distance PCR(LD-PCR).
The results showed that total RNA production of wheat leaves(μg/100 mg leaves) extracted by Trizol,Licl,SDS and Bizol protocols were 4.72,1.15,1.56,10.43 kg/100 mg,respectively,total RNA production of wheat leaves extracted by Trizol and Bizol protocols were obviously higher than that by Licl and SDS protocols.
cDNA synthesized from RNA extracted by Trizol was mainly 0.25~2.0 kb,cDNA synthesized from RNA extracted by Bizol was mainly 0.5~5.0 kb,cDNA synthesized from RNA extracted by Bizol was better than that by Trizol.
Ultrasonic-assisted extraction was used to extract solanesol from tobacco leaves,the optimum extraction parameters were chosen as follows by orthogonal design: acetone as solvent,temperature 60 ℃,extraction time 2 h,ratio of leaves: solvent at 1∶ 17.5 and ultrasonic power 160 W,extraction time was the key factor affect the extraction rate.
In this paper, the approach of microwave is adopted to extract the flavonol from roses with large petals and small ones as well as make available their respective content of flavone: 71.4736mg/g and 71.0636mg/g, with the RSD(n=9) of 0.5972% and 0.5852% respectively.
Pseudo-Jacobi(p=4,q=3)-Fourier Moments(PJFM's) were applied to extract image featurs from six microscopic characteristic original photos of flos carthami,which included farina,fragment of secretory pipe,top cell of petal,petal cell,stigma cell,middle cell of anther in this paper.