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  extraction
    STUDY ON THE EXTRACTION OF HIGH-MOLECULARWEIGHT DNA FROM Pleurotus ostreatus
    糙皮侧耳高分子量DNA的提取研究
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    The extraction and protection of enzyme activity of bromelin from organs of pineapple
    菠萝各器官蛋白酶的提取与保活
短句来源
    A New Method of DNA Extraction for Identificationof Transgenic Plants with PCR
    以PCR鉴定转基因植株的微量DNA提取方法
短句来源
    Genomic DNA Extraction and RAPD Analysis of Eggplant and Its Wild Relatives
    几种茄属植物基因组DNA提取与RAPD分析
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    The optimum conditions for flavoids extraction were as follow: the ratio of solid to liquid was 1∶30 with 50% ethanol,microwave time was 2 min,extraction time was 1 h and temperature was 80 ℃.
    实验结果表明:以体积分数50%乙醇水溶液作提取溶剂,固液比1∶30(g/mL),微波照射2min,浸提时间1h,提取温度80℃,苦瓜叶中黄酮提取率最高。
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  from
    STUDY ON THE EXTRACTION OF HIGH-MOLECULARWEIGHT DNA FROM Pleurotus ostreatus
    糙皮侧耳高分子量DNA的提取研究
短句来源
    The extraction and protection of enzyme activity of bromelin from organs of pineapple
    菠萝各器官蛋白酶的提取与保活
短句来源
    Three clear bands of 28S,18S and 5S could be seen clearly by agarose gel electrophoresis and no degradation was observed. The yield of isolated RNA was high and from 100 μg to 150 μg RNA per 0.1 grams of young leaves could be harvested.
    对Trizol试剂提取总RNA的方法进行了改进,所提RNA经琼脂糖电泳检测,其28S、18S、5S 3条带清晰,完整性好,每0.1g幼叶可获得RNA 100~150μg,产率高;
短句来源
    The contents of C,H,O and methoxyl groups of MWL the purified milled wood lignin(MWL) extracted from walnut shell(Juglans sigillata) were determined and the basic chemical groups of MWL were characterized by 1H-NMR.
    在核桃壳磨木木质素(MW L)提取纯化的基础上,对纯化的核桃壳磨木木质素进行了C、H、O及OCH3含量的测定,并利用1H-NMR对核桃壳木质素基本化学结构进行研究。
短句来源
    The optimum extracting conditions of flavonoids from dry celery and west celery were studied with the ethanol concentration 40 %(mass concentration),temperature 70~80 ℃,solid-liquid ratio 1 g∶15 ml and extraction time 3 h.
    采用回流提取法,研究了乙醇浓度、温度、料液比、提取时间对旱芹和西芹中黄酮类物质提取效率的影响规律。 确定其最佳提取工艺为乙醇质量浓度40%、温度70~80℃、料液比1 g∶15 ml、提取3 h。
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  extracted
    The contents of C,H,O and methoxyl groups of MWL the purified milled wood lignin(MWL) extracted from walnut shell(Juglans sigillata) were determined and the basic chemical groups of MWL were characterized by 1H-NMR.
    在核桃壳磨木木质素(MW L)提取纯化的基础上,对纯化的核桃壳磨木木质素进行了C、H、O及OCH3含量的测定,并利用1H-NMR对核桃壳木质素基本化学结构进行研究。
短句来源
    The result showed as follows:by raising the concentrations of antioxidative components and antiphenolic compounds(2% β-ME,2% PVP-40,20 mmol/L Na2S2O5),the purity of the extracted genomic DNA was higher.
    结果表明,适当提高抗氧化、抗酚类物质的浓度(2%β-巯基乙醇,2%PVP-40,20 mmol/LNa2S2O5),提取的基因组DNA纯度较高;
短句来源
    Total DNA was extracted from the raspberry young leaves and the different extraction methods were compared,which tried to find out the suitable method for the raspberry genomic total DNA extraction.
    以树莓嫩叶为材料,进行树莓基因组总DNA不同提取方法的比较试验。
短句来源
    The result indicated that the CTAB method,the SDS method,the improved CTAB method may successfully extract DNA,but regardless of the quality and the quantity,the improved CTAB method surpassed conventional CTAB,SDS,DNA extracted mostly presented the colorless transparency by the improved CTAB method,The A260/ A280 values was between 1.65~1.90,This quality of DNA was suitable for RAPD analysis.
    结果表明:CTAB法、SDS法、改良CTAB法均可成功提取树莓DNA,但无论在质量上还是在数量上,改良CTAB法都优于常规CTAB法和SDS法,所提取的DNA大多呈现无色透明状,A260/A280值多数介于1.65~1.90,完全可以用于RAPD分析。
短句来源
    The DNA samples extracted by four methods were tested by agarose gel electrophoresis,ultraviolet spectrophotometer,PCR(RAPD and ISSR),and digested with restriction enzymes(EcoRI and PstI).
    通过琼脂糖凝胶电泳、紫外分光光度计、RAPD、ISSR等方法进行PCR扩增和用EcoRI、PstI内切酶酶切对所提取的DNA样品进行检测,比较所得DNA的产量和质量。
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  extract
    Submerged Cultivation of Pleurotus djamour in Peat Extract for the Production of Mushroom Mycelium
    食用菌Pleurotus djamour在泥炭提取液中的深层培养
短句来源
    Tested with Yali pear and Betulaefolia,the paper studied the extract of DNA of Pyrus and the optimized RAPD reaction system.
    以鸭梨、杜梨为材料,对梨属植物DNA的提取及RAPD反应体系进行了系统优化,建立了梨属植物最佳反应体系及参数。
短句来源
    It explained that reformed CTAB method could extract the genomic DNA of Pyrus successfully,and the optimum RAPD reaction system could amplificate RAPD in Pyrus L..
    说明改良的CTAB法能成功用于梨属植物DNA提取,建立的最佳反应体系可用于梨属植物RAPD扩增。
短句来源
    Six cultivars of Zihua, Bayuelu, Jiangjun, 73-8, 96-9, and 9z622 were used to extract high-quality genomic DNA by using the improved SDS method.
    以紫花、八月绿、将军、73-8、96-9、9z622等6个普通菜豆品种为试材,采用改良的SDS法提取了高质量的基因组DNA。
短句来源
    The result indicated that the CTAB method,the SDS method,the improved CTAB method may successfully extract DNA,but regardless of the quality and the quantity,the improved CTAB method surpassed conventional CTAB,SDS,DNA extracted mostly presented the colorless transparency by the improved CTAB method,The A260/ A280 values was between 1.65~1.90,This quality of DNA was suitable for RAPD analysis.
    结果表明:CTAB法、SDS法、改良CTAB法均可成功提取树莓DNA,但无论在质量上还是在数量上,改良CTAB法都优于常规CTAB法和SDS法,所提取的DNA大多呈现无色透明状,A260/A280值多数介于1.65~1.90,完全可以用于RAPD分析。
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