A/tiger/Harbin/01/2003(H5N1)HA gene was cloned into PVAX1.The HA expression cassette which included CMV and HA and PolyA was ligated into the E3 deletion region of pVAXΔE. The recombinant plasmid was named pΔ EHA.
2. Construction of recombinant fowlpox virus expressing hemagglutinin gene of subtype H5 avian influenza virusHemagglutinin gene of subtype H5 avian influenza virus was amplified by PCR to construct expression cassette containing FPV early and late promoter and SV40 polyA tail.
To differentiate and detect the significant subtype of avian influenza virus rapidly, sensitively and distinctly, Based on their conserved regions of HA genes of H5、H7and H9 subtype of avian influenza virus registered in GenBank,three sets of specific primers were designed and synthesized.
Distribution of Avian Influenza of Viruses of Different HA Subtypes Among Domestic Ducks in Eastern China and Molecular Epidemiology of HA Genes of H5 Subtype Avian Viruses Isolated from Domestic Waterfowls and Chickens
The protective efficacy of a plasmid DNA that contains two AIV HA genes was evaluated in SPF chickens. Two AIV HA genes of H5 and H7 subtype were cloned into the same vector,thus a dou-blegene vector-pCI-H5HA-H7HA had been constructed successfully.
In order to study HA gene vaccine, the H5 gene of AIV, amplified by PCR, was subcloned into eukaryotic expressing vectors pcDNA4/HisMax and pRc/CMV. The recombinant plasmids were transfected into Hela cell by Tfx?
In order to study HA gene vaccine, the H5 gene of AIV, amplified by PCR, was subcloned into eukaryotic expressing vectors pcDNA4/HisMax and pRc/CMV. The recombinant plasmids were transfected into HeLa cell by Tfx TM-20 transfection reagent, Superfect transfection reagent and electroporation respectively.
The H5 gene of AIV, A/Goose/GuangDong/1/96(H5N1), was subcloned into eukaryotic expressing vectors pcDNA4/ HisMax and pRc/ CMV, and these construct were designated pC4H5 and pCMVH5. SPF chickens were immunized using electroporation by two injections, 3 weeks apart, of 30μg /ck or 50μg/ck into the right quadriceps muscle.
Endosperm hardness in wheat (Triticum aestivum L.) is determined by one major genetic factor, the Hardness (Ha) gene on the short arm of chromosome 5D.
The transfection of murine SP1 tumor cells with the hemagglutinin (HA) gene of influenza virus results, after fluorescent-activated cell sorting (FACS), in the selection of high-HA-expressing cell lines called H4A and H4B.
Our results suggest that molecular analysis of the HA gene is important to complement the antigenic characterization for a better selection of appropriate vaccine strains.
By replacement of the HA gene of human strains new pandemic viruses can be generated (antigenic shift).
The expression of the AtP2C-HA gene is up-regulated by abscisic acid (ABA) treatment.
Measles virus (Nepal strain) hemagglutinin gene: Cloning, complete nucleotide sequence analysis and expression in COS cells
Hemagglutinin gene of Measles virus(Nepal strain) was amplified by RT-PCR technique, cloned and sequenced by the dideoxy-mediated chain termination method.
Measles virus strains isolated in Japan since 1984 were classified into the genotypes C1 (-1985), D3 (1985-1990), D5 (1990-1997), and Chicago-type D3 (1997-1999) from the results of sequencing the hemagglutinin gene.
This means replacement of at least the hemagglutinin gene of the prevailing human strain by the allelic gene of an avian influenza virus by reassortment.
Structure of the hemagglutinin gene of influenza virus during serial passage through chick embryos