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细菌重组
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  “细菌重组”译为未确定词的双语例句
     Construction of adenovirus vector carrying murine Flt3 ligand by using bacterial homologous recombination system and its expression in hepatocellular carcinoma
     应用细菌重组系统构建小鼠Flt3配体的重组腺病毒及其在肝癌细胞中的表达
短句来源
     Expression of Crg-2 by a bacteria recombination system of adenovirus
     腺病毒细菌重组系统表达Crg-2蛋白的实验研究
短句来源
     The Research Progress of Recombinant Attenuated Salmonella typhimurium Vaccine of Bacteria
     细菌重组减毒鼠伤寒沙门氏菌疫苗研究进展
短句来源
     Methods:1 First, we get the gene CD and the gene segment gly ES from the recombinant adenovirus rAdCD and the secretory plasmid pEZZ-18-ES which have been constructed by our department by PCR. Then we constructed the recombinant adenovirus plasmid containing CDglyES fusion gene and got the recombinant adenovirus by amplified them in the 293 cells. And also verified the activities of CD and ES genies respectively in CDglyES fusion gene.
     1 首先从本课题组前期构建的重组腺病毒rAdCD和pEZZ-18-ES分泌型表达质粒中,用PCR法获取CD和glyES基因,然后用细菌重组的方法构建含CDglyES融合基因的重组腺病毒质粒pAdCDglyES,在293细胞中扩增获得重组腺病毒rAdCDglyES并在体外证实融合基因CDglyES中的两个基因有独自生物学活性。
短句来源
     In this study, the cDNA fragments containing Glycoprotein gene of rabies ERA strain was amplified by reverse transcription-polymerase chain reaction (RT-PCR) with primers ERG1/ERG2, and then the cDNA fragment was cloned to PMD-18T.
     现代分子生物学的发展,给口腔常在细菌重组疫苗的开发创造了有利的条件。 本实验设计引物ERG1/ERG2,用RT-PCR方法扩增出了狂犬病疫苗株ERA株G基因。
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     The recombinant plasmid expressed well in E.
     从重组E.
短句来源
     Bacterial clone of recombinant DNA of DHCCR
     DHCCR重组DNA细菌克隆
短句来源
     Detection of bacterial endotoxin in recombinant human cytokine products
     重组人细胞因子制品细菌内毒素的检测
短句来源
     Lysogenic bacteria E.
     溶源性细菌E.
短句来源
     Specific enzymatic Activity of glycerol dehydratase in E.
     重组菌E.
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  bacterial recombination
Evolution of sexual isolation is not a milestone toward speciation in bacteria, since bacterial recombination is too rare to oppose adaptive divergence between incipient species.
      


Objective To construct the recombinant adenovirus vector carrying murine Flt3 ligand (AdmFL) and detect the expression of mFL in Hepa1 6 cells following infection by AdmFL. Methods We amplified mFL from the plasmid and constructed the recombinant adenovirus vector using a new simplified bacterial homologous recombination system, then infected Hepa1 6 cells and detected the expression of mFL by RT PCR. Results] We successfully constructed the recombinant adenovirus vector carrying mFL and detected its expression...

Objective To construct the recombinant adenovirus vector carrying murine Flt3 ligand (AdmFL) and detect the expression of mFL in Hepa1 6 cells following infection by AdmFL. Methods We amplified mFL from the plasmid and constructed the recombinant adenovirus vector using a new simplified bacterial homologous recombination system, then infected Hepa1 6 cells and detected the expression of mFL by RT PCR. Results] We successfully constructed the recombinant adenovirus vector carrying mFL and detected its expression in infected Hepa1 6 cells. Conclusions he construction of adenovirus vector carrying mFL paves the way for further application in cancer gene therapy.

目的 构建携带小鼠Flt3配体 (murineflt3ligand ,mFL)的重组腺病毒载体并检测它在感染后的小鼠肝癌细胞中的表达 ,为肝癌基因治疗提供实验基础。方法 PCR扩增含有mFL的质粒 ,应用高效细菌内同源重组系统构建携带mFL的重组腺病毒 ,感染小鼠肝癌细胞株Hepa1 6 ,一步法提取细胞总RNA ,RT PCR检测mFL的表达。结果 构建了携带mFL的重组腺病毒 ,并在感染后的Hepa1 6细胞中检测到了mFL的mRNA。结论 应用该细菌重组系统成功地构建了携带mFL的重组腺病毒载体 ,为下一步的基因治疗研究奠定了基础

Salmonella typhimurium is one type of enteric pathogenic bacteria.Many attenuated strains such as X4072,X4217,X4550,X4989,X4990,X4064,SL3261,GID101,GID105,GID106 and BRD509 were constructed by gene mutation;which are precise mutation of two or many gene.Their genotype and phenotype are very stable,so they are acted as expression vector to make bacterial vaccine.Many recombinant attenuated Salmonella typhimurium vaccine of bacteria have been studied in succession,those bacteria include Streptococcus pneumoniae...

Salmonella typhimurium is one type of enteric pathogenic bacteria.Many attenuated strains such as X4072,X4217,X4550,X4989,X4990,X4064,SL3261,GID101,GID105,GID106 and BRD509 were constructed by gene mutation;which are precise mutation of two or many gene.Their genotype and phenotype are very stable,so they are acted as expression vector to make bacterial vaccine.Many recombinant attenuated Salmonella typhimurium vaccine of bacteria have been studied in succession,those bacteria include Streptococcus pneumoniae , Mycobacteria tuberculosis , Bordetella pertussis , Listeria monocytogenes , Yersinia pestis , Francisella tularensis , Pseudomonas aeruginosa , Neisseria gonorrhoeae , Brucella abortus , Clostridium tetanus, Bacillus anthracis , Chlamydia trachomatis and Mycoplasma hyopneumoniae .The construction and its immune mechanism of those vaccine were emphasized to discuss in this review.

鼠伤寒沙门氏菌 ( St)是一种肠道致病菌 ,通过基因突变可构建许多鼠伤寒沙门氏菌减毒株 ,例如 :X40 72、X42 17、X45 5 0、X4989、X4990、 X40 64、 SL 3 2 61、 GID10 1、 GID10 5、GID10 6和 BRD5 0 9等 ,它们均是两个或多个基因的精确突变 ,其基因型和表型均很稳定 ,可用作构建细菌性疫苗的表达载体。国内外学者相继研制了肺炎链球菌、结核杆菌、百日咳杆菌、产单核细胞李斯特菌、鼠疫耶尔森菌、土拉弗郎西斯菌、铜绿假单胞菌、淋病奈瑟菌、牛布氏杆菌、破伤风梭菌、炭疽芽孢杆菌、沙眼衣原体和猪肺炎支原体等细菌的重组减毒鼠伤寒沙门氏菌疫苗。文章着重讨论了这些疫苗的构建及其免疫机制等方面的研究进展 ,从而为新型菌苗的研制提供一种新的思路

Objective: To purify recombinant Paragonimus Westermani(Pw) adult antigen expressed in bactieria Pw-1/pRSET B/BL 21. After renature, the purified products were applied to detect the sera collected from paragonimiasis patients, other parasitosis and normal persons with ELISA and the specificity, sensitivity and application prospect of ELISA using Pw were evaluated. Methods: After primary purification of the recombinent protein by dialyse, the primary purified protein was further purified by Fast Protein Liquid...

Objective: To purify recombinant Paragonimus Westermani(Pw) adult antigen expressed in bactieria Pw-1/pRSET B/BL 21. After renature, the purified products were applied to detect the sera collected from paragonimiasis patients, other parasitosis and normal persons with ELISA and the specificity, sensitivity and application prospect of ELISA using Pw were evaluated. Methods: After primary purification of the recombinent protein by dialyse, the primary purified protein was further purified by Fast Protein Liquid Chromatography(FPLC), then the effects of the two steps of purification methods were compared. The primary purified products by Oxidation/reduction GSH renatured were to abtain the recombinant protein antigen. Finally, the sera collected from patients of paragonimiasis, schistosomiasis, clonorchiasis sinensis, cysticerciasis, echinococcosis and normal persons were detected for corresponding antibodies against the recombinant protein antigen and the Pw adult crude antigen respectively by ELISA. Results: FPLC, the sensitivity of the recombinant antigen tested by paragonimiasis patients'sera was 91.07%, and there was not any cross reaction with the sera collected from other parasitosis and normal persons. In contrast, the sensitivity of crude antigen was 100%, while the percentages of cross reaction were as follows: schistosomiasis 11.43%, clonorchiasis sinensis 4.84%, cysticerciasis 3.22%, echinococcosis 5.88%, normal person 2.5%. Conclusion: The primary purified antigen applied in the immunodiagnosis has higher specificity.

目的纯化重组细菌Pw鄄1/pRSETB/BL21的表达产物肺吸虫重组抗原,复性后以ELISA法检测肺吸虫病患者、其他寄生虫病患者和正常人血清,评价其敏感性、特异性和应用前景。方法通过分步洗涤及透析对细菌重组蛋白进行初步纯化,进一步做快速液相层析纯化,比较前后两法的纯化效果。初步纯化产物经氧化/还原型谷胱苷肽复性后即为重组蛋白抗原(以下简称重组抗原)。用该重组抗原,以ELISA法检测肺吸虫、血吸虫、肝吸虫、囊虫和包虫病患者以及正常人血清中相应抗体,并与卫氏肺吸虫粗抗原做比较。结果经分步洗涤及透析纯化后的重组抗原纯度已较高,快速液相层析进一步纯化效果不明显。以初步纯化后经复性的重组抗原进行ELISA法检测肺吸虫病患者血清,敏感性为91.07%,与其他寄生虫病患者和正常人血清均无交叉反应;粗抗原检测肺吸虫病患者血清敏感性虽为100%,但与其他寄生虫病患者和正常人血清交叉反应率分别为血吸虫病11.43%,肝吸虫病4.84%,囊虫病3.22%,包虫病5.88%,正常人为2.5%。结论研究制备的重组抗原应用于肺吸虫病的免疫诊断特异性较高。

 
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