The specificity of the primer pairs were tested by sequence analysis of the PCR product. Real-time PCR were applied for quantifying AU126 in patients with aggressive periodontitis(n=17),chronic periodontitis(n=25)and plaque-induced gingivitis(n=26). Subjects with healthy periodontal tissue were served as normal controls(n=38).
Results It was shown by quantitative real-time PCR that AU126 in subgingival plaque of aggressive periodontitis(8.72±0.44),chronic periodontitis(8.46±0.67) and plaque-induced gingivitis patients(8.47±0.47) were higher than that in normal controls(7.91±0.88)(P<0.05).
METHODS: Ninety-one patients with chronic adult periodontitis (AP) and 41 cases with healthy periodontiwm were selected, the oral hygiene and caries condition were detected.
Patients with untreated chronic periodontitis underwent subgingival debridement alone or in combination with adjunctive antimicrobial therapy (systemic amoxicillin and metronidazole/7?days plus supragingival CHX irrigation).
Eight patients with chronic periodontitis and each with one very deep intrabony defect around a tooth scheduled for extraction were treated with either a combination of BDX + GTR (five patients) or with BDX?Coll + GTR (three patients).