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     its ovum livesthrough the winter;
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     The nucleus is egg-shaped.
     细胞核圆形。
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A single dose of 100 Y of x-rays was given to the female albino mice (C-I) at different ages between newborn to 38-days old.The degree of radiation injuries and the radiation sensitivity of the ovary decrease as the age of the animal increases.Radiation sensitivity differs in the different types of follicles and in their different developmental stages too.And the injurious pictures differ in the different types of follicles.However,no significant morphological difference could be found between the injures follicles...

A single dose of 100 Y of x-rays was given to the female albino mice (C-I) at different ages between newborn to 38-days old.The degree of radiation injuries and the radiation sensitivity of the ovary decrease as the age of the animal increases.Radiation sensitivity differs in the different types of follicles and in their different developmental stages too.And the injurious pictures differ in the different types of follicles.However,no significant morphological difference could be found between the injures follicles of the exposed mice and the senile follicles of the controls.The number of the injured oocytes in the ovaries of the x-radiation newborn mice increases rapidly as the dose of x-rays (within the range of 5-50 r) increases.

出生后38天内不同年龄的中国一号品系雌性小鼠,经100伦琴的X射线照射后24小时,卵巢的损伤程度随着小鼠年龄的增大而放射敏感性相应地降低,卵巢发育不同阶段中不同类型的滤泡具有不同的放射敏感性,并且不同类型的滤泡具有不同的损伤景象。但对照小鼠卵巢中的退化滤泡与照射组中的损伤滤泡并无可观察到的形态学方面的差别,出生当天小鼠原始卵母细胞的损伤数随着照射剂量的增加(5—50伦琴)而急剧地升高。

Sex may differentiate in European eel at the stage of glass eel become black eel, about 10.5 ~ 12.0cm in body length. The observation of gonadal sections indicate that prlmitive oogonia in the female ovary and spermatogonia in the male testis are found at this stage. It in-dicates that sex differentiation in the European eel is earlier than that of Japanese eel. The re-sults of observation of gonadal sections. of slow growing eel collected from two eel farms in FuJian province indicate that all slow growing...

Sex may differentiate in European eel at the stage of glass eel become black eel, about 10.5 ~ 12.0cm in body length. The observation of gonadal sections indicate that prlmitive oogonia in the female ovary and spermatogonia in the male testis are found at this stage. It in-dicates that sex differentiation in the European eel is earlier than that of Japanese eel. The re-sults of observation of gonadal sections. of slow growing eel collected from two eel farms in FuJian province indicate that all slow growing eel,except specitic individuals,were male. It is pointed out that a cause of slow growing in Eeuropean eel may be realated with sex. Finally,the complex factor inducing slow growing of eel is discussed in this paper.

欧洲缝纫性分化时间在白仔鳗缓转变为黑仔鳗时期,体长10.5~12.0cm。性腺切片观察可见雌性卵巢中有原始卵原细胞,雄性生材小管中有精原细胞,性别分化明显早于日本鳗鲡。从福建省两个养鳗场采集僵鳗,取其性腺切片观察结果表明,除个别僵鳗外,全部为雄性,提示僵鳗成因可能与性别有关。文中讨论了发生僵鳗的复杂因素。

Purpose To investigate whether the oval cells exist in human hepatocellular carcinoma (HCC). Methods Surgical specimens from 20 cases of human HCC were investigated by light microscopy, electron microscopy, and immunostaining for cytokeratin 7 (a marker of biliary differentiation), and albumin (a marker of hepatocytic differentiation). Double labeling for these two antigens was carried out by immunoelectron microscopy in 5 cases. Results Proliferating bile ductules were noted around the edges of tumors in...

Purpose To investigate whether the oval cells exist in human hepatocellular carcinoma (HCC). Methods Surgical specimens from 20 cases of human HCC were investigated by light microscopy, electron microscopy, and immunostaining for cytokeratin 7 (a marker of biliary differentiation), and albumin (a marker of hepatocytic differentiation). Double labeling for these two antigens was carried out by immunoelectron microscopy in 5 cases. Results Proliferating bile ductules were noted around the edges of tumors in 14 of 20 cases. Electron microscopy revealed three types of oval cells. Type Ⅰ cells were small, primitive oval cells with a high nucleocytoplasmic ratio. Type Ⅱ cells, which exhibited differentiation towards biliary epithelial cells, were larger and exhibited more cytoplasm. Type Ⅲ cells exhibited similarities with hepatocytes,which were larger and contained more rough endoplasmic reticulum. Intracellular tonofilaments and intercellular junctions were seen in all three types of oval cells. By immunoelectron microscopy all three types of oval cells were found to express cytokeratin 7 and albumin, although labeling for cytokeratin 7 was stronger in types Ⅰ and Ⅱ, and labeling for albumin was stronger in type Ⅲ. Conclusion Oval cells that exhibit the same morphologic and immunophenotypic features as those seen during animal hepatocarcinogenesis are found in human HCC. The findings support the hypothesis that oval cells are hepatic progenitor cells.

目的 :探讨人肝癌肝组织存在卵圆细胞的可能性。方法 :对 2 0例人肝细胞肝癌的手术标本行常规组织学和超微结构观察 ,并用胆管上皮分化标记CK7和肝细胞分化标记白蛋白对以上组织作免疫组化染色 ,同时对其中 5例作免疫电镜标记。结果 :光镜下 ,14/ 2 0例癌肿边缘常可见到增生的小胆管样结构。电镜下 ,14/ 2 0例可找到三型卵圆细胞。其中Ⅰ型细胞体积较小、核大、胞质少 ,此为较为原始的卵圆细胞。Ⅱ型细胞体积稍大 ,胞质稍多 ,此为向胆管上皮分化的小上皮细胞。Ⅲ型细胞体积稍大 ,内含稍多粗面内质网 ,此为向肝细胞分化的卵圆细胞。此三型卵圆细胞均有细胞内张力微丝和细胞间连接结构。免疫电镜显示 ,三型卵圆细胞均表达CK7和白蛋白 ,但Ⅰ型和Ⅱ型表达CK7多些 ,Ⅲ型表达白蛋白多些。结论 :与动物致癌模型肝中存在卵圆细胞一样 ,人肝细胞肝癌肝中也存在同样形态和免疫表型特点的卵圆细胞。结果支持卵圆细胞可能为肝前体细胞的假设。

 
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