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黄化法
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     A NOVEL METHOD BASED ON ETIOLATION FOR INCREASING EFFICIENCY OF IN VITRO PROPAGATION OF POPULUS TOMENTOSA
     一种以黄化法为基础提高毛白杨快速繁殖效率的新方法
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     This paper describes a novel method based on etiolation treatment for micropropagation of Populus omentosa.
     本文报道以黄化法为基础的毛白杨(Populus tomentosa)快速繁殖方法的初步研究结果。
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     The xanthate derivative method,halogenated solvent method and amide LiCl method for preparing chitin fiber are introduced.
     介绍了制备甲壳素纤维的黄化法、含卤溶剂法和酰胺 氯化锂法 .
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     It provides effective support for our building of rule of law.
     其次,
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     On Mediation Method
     论和
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     A NOVEL METHOD BASED ON ETIOLATION FOR INCREASING EFFICIENCY OF IN VITRO PROPAGATION OF POPULUS TOMENTOSA
     一种以黄化为基础提高毛白杨快速繁殖效率的新方
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This paper describes a novel method based on etiolation treatment for micropropagation of Populus omentosa.It includes:(1)Antibiotic“cefotaxime”(2.5 ppm in nutrient medium)is used to inhibit bacteria growth and the cultures which have been contaminated after long pe- riod subculture are recovered by sterilization with 1/10000 HgCl_2(w/v).(2)Low concentration of TDZ(thidiazuron,0.005 ppm)replaces expensive zeatin for enhancing bud differentiation and multiplication of leaf explants.(3)Shoot elongation is promoted...

This paper describes a novel method based on etiolation treatment for micropropagation of Populus omentosa.It includes:(1)Antibiotic“cefotaxime”(2.5 ppm in nutrient medium)is used to inhibit bacteria growth and the cultures which have been contaminated after long pe- riod subculture are recovered by sterilization with 1/10000 HgCl_2(w/v).(2)Low concentration of TDZ(thidiazuron,0.005 ppm)replaces expensive zeatin for enhancing bud differentiation and multiplication of leaf explants.(3)Shoot elongation is promoted after etiolation treat- ment,which will increase the number of shoots suitable for rooting,about 50 etiolated shoots are obtained in a 100 ml flask,3—5 times more than those produced in traditional method.(4) Etiolated shoots or after their greening are used as cuttings for rooting in vivo and over 90% survival rate could be achieved when the medium is sterilized.This method is labour and money saving and high efficient.

本文报道以黄化法为基础的毛白杨(Populus tomentosa)快速繁殖方法的初步研究结果。它包括1.用低浓度的先锋霉素(cefotaxime 2.5ppm)抑制快速繁殖过程中的细菌污染和用低浓度 HgCl_2灭菌的方法恢复经长期继代培养后被污染的试管植物;2.用极低浓度的 TDZ(thidiazuron 0.005ppm)替代价格昂贵的玉米素(zeatin,Z),促进毛白杨叶外植体上芽的分化和增殖;3.用黄化的方法促使芽的伸长以增加可被用于生根的嫩枝的数量。用这种方法可在一个100ml 的三角瓶中得到约50个黄化的嫩枝,比在光下培养得到的嫩枝多3—5倍;4.黄化嫩枝直接扦插或经光下转绿后扦插于经灭菌的基质中,生根率均可达到90%以上。这一方法可以节省人力和能耗,提高培养室的利用效率,大幅度地提高快速繁殖的效率。

The preparation and properties of chitin based fibers are reviewed.The xanthate derivative method,halogenated solvent method and amide LiCl method for preparing chitin fiber are introduced.The normal methods for preparing chitosan fiber and the properties of chitosan fiber are summarized.The fibers made from chitin/chitosan derivatives (both esters and ethers) are briefly described.The application of these fibers is discussed.

:综述了甲壳素及其衍生物纤维的制备和性能 .介绍了制备甲壳素纤维的黄化法、含卤溶剂法和酰胺 氯化锂法 .总结了壳聚糖纤维制备的一般方法和性能 .简要介绍了甲壳素 /壳聚糖酯类和醚类衍生物的纤维 .讨论了甲壳素类纤维的应用

 
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