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扣除杂交
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  subtractive hybridization
     In order to analyze the molecular mechanism for salt tolerance in P Euphratica, salt-related genes were screened through Suppressed Subtractive Hybridization (SSH) with the results as follows:
     为了研究胡杨耐盐的分子机制,通过抑制性扣除杂交(Suppressed subtractive hybridization, SSH)技术从胡杨中筛选与耐盐性相关的基因。 结果如下:
短句来源
     A vacuolar ATPase (V_ATPase) B subunit gene has been cloned and characterized from a phosphorus starvation induced rice root subtractive cDNA library by suppression subtractive hybridization (SSH) method and RT_PCR amplification.
     利用抑制性扣除杂交 (SSH)技术构建水稻 (OryzasativaL .) 根系磷饥饿诱导cDNA文库 ,获得编码液泡ATPase (V_ATPase)B亚基的克隆 ,通过反转录PCR方法获得该基因的完整序列。
短句来源
     To identify differentially expressed genes between fetal mesenchymal stem cell (MSC) and adult MSC, especially specified genes expressed in fetal MSC, a cDNA subtractive library of fetal MSC was constructed using suppression subtractive hybridization (SSH) technique.
     为了获得胎儿和成人间充质干细胞 (mesenchymalstemcells,MSC)的差异表达基因 ,尤其是在胎儿MSC中特异表达的基因 ,应用抑制扣除杂交技术构建了胎儿和成人MSCcDNA扣除文库。
短句来源
     The target DNA sequences were obtained by subtractive hybridization with Representational Difference Analysis (RDA) and PCR, and retrieved with the low melting point gel.
     利用代表性差异分析 (RDA)及 PCR进行扣除杂交 ,获得目的 DNA片段 ;
短句来源
     Advances in the Study on the Suppression Subtractive Hybridization
     抑制性扣除杂交技术(SSH)及其在基因克隆上的研究进展
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  “扣除杂交”译为未确定词的双语例句
     Through the subtraction, a wild plant specific genome DNA fragment has been found and this fragment will be used as a probe in following mutant gene screening study.
     应用扣除杂交技术对该变异株和野生型植株进行了基因组差示杂交 ,获得样本特异DNA片段 ,此DNA片段可以作为进一步筛选突变基因的探针 ,为克隆文冠果花发育调控基因奠定基础
短句来源
     The differential display reverse transcription-PCR and suppressed-subtractive-hybridi have been extensively used in the difference analysis of genetic expression because of their high sensitivity and lower fasle positive rate. This paper outline their principle, technique, advantage, disadvantage and application in genetic express and difference analysis.
     差异显示反转录 -PCR和抑制性扣除杂交是目前广泛应用于基因表达差异分析的方法 ,它们分别具有灵敏度高假阳性率低等特点 ,对其原理、技术、优缺点及在基因表达差异分析中的应用进行简要概述
短句来源
     In this paper,in combination of the experiment of SSH seiving genes conected with arsenic metabolism, the general principle,procedure,advantages and shortcomings are summarized.
     结合进行的用SSH法筛选与砷代谢相关的实验 ,主要介绍了抑制性扣除杂交技术的主要原理、基本过程、优越性、主要缺陷及目前最新的研究进展
短句来源
     With the analysis of ESTs from rice Nipponbare cDNA library screened by a method of Suppression subtracted hybridization (SSH), the phosphate-induced genes OsIPSl and OsIPS2 were cloned in this research. OsIPS2 has been reported as OsPIl by Wasaki et al.
     本研究通过对水稻日本晴cDNA扣除杂交库中ESTs的筛选结果进行分析,克隆了磷饥饿诱导基因OsIPS1与OsIPS2,其中OsIPS2(OsPI1)已于2003年被Wasaki等报道。
短句来源
     The Technique of Differential Display Reverse Transcription-PCRand Suppressed-subtractive-hybrid(SSH) and Their Application in the Difference Analysis Genetic Expression
     差异显示反转录-PCR及抑制性扣除杂交在基因表达差异分析中的应用
短句来源
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  相似匹配句对
     e-Hybrid Rice
     e杂交
短句来源
     northern hybridization.
     Northern杂交
短句来源
     The Methods and Applications of Differential Subtractive Hybridization in Molecular Biology
     示差扣除杂交法及其在分子生物学中的应用
短句来源
     Separation of gene clones related to crocin synthesis using subtractive hybridization
     用扣除杂交法分离藏红花苷合成相关基因的克隆
短句来源
     There were little amount MgO impurity phase in this sample.
     扣除MgO的影响。
短句来源
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  subtractive hybridization
Interspecies Subtractive Hybridization of cDNA from Human and Chimpanzee Brains
      
A New Planarian Extrachromosomal Virus-like Element Revealed by Subtractive Hybridization
      
A combination of suppression subtractive hybridization and a new technique of mirror orientation selection was used to compare the total DNA for two, sexual and asexual, races of freshwater planarian Girardia tigrina.
      
In this study, we adopted the suppression subtractive hybridization (SSH) technique to isolate differentially expressed ESTs from Gossypium barbadense variety 7124 during the Verticillium wilt defense process.
      
Isolation by suppression-subtractive hybridization of genes preferentially expressed during early and late fiber development sta
      
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  subtractive hybridization (ssh
In this study, we adopted the suppression subtractive hybridization (SSH) technique to isolate differentially expressed ESTs from Gossypium barbadense variety 7124 during the Verticillium wilt defense process.
      
Suppression subtractive hybridization (SSH) was shown to be suitable for generating a set of DNA fragments differing between two closely related bacterial strains.
      
To identify rice genes induced early in the defense response, suppression subtractive hybridization (SSH) was used to generate a cDNA library enriched for transcripts differentially expressed during infection by R.
      
pestis Antiqua strain 49006 using a combination of suppression subtractive hybridization (SSH) and comparative genomic hybridization with DNAs from a diverse panel of Y.
      
Recently, a new and highly effective method termed suppressive subtractive hybridization (SSH) has been introduced to clone differentially expressed mRNAs.
      
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Recently, several new methods about isolating and cloning plant developmental genes have been reported. mRNA differential display is widely used as a speedy and dramatic method, because it only needs very small amount of starting materials and can be used to compare many treatments simultanously. But it has two drawbacks, the high frequency of false positives and the short size of differentially displayed fragments. To overcome these drawbacks, two new methods, called representational difference analysis (RDA)...

Recently, several new methods about isolating and cloning plant developmental genes have been reported. mRNA differential display is widely used as a speedy and dramatic method, because it only needs very small amount of starting materials and can be used to compare many treatments simultanously. But it has two drawbacks, the high frequency of false positives and the short size of differentially displayed fragments. To overcome these drawbacks, two new methods, called representational difference analysis (RDA) and suppression subtractive hybridization (SSH), are pointed out. In this paper, we discuss in detail the disadvantages of mRNA differential display and the advantages of RDA and SSH. On the other hand, we draw out the progress of these three methods.

过去20年,人们已经分离到了相当数量的与发育相关的基因。这主要借助于蛋白质产物的分离纯化,然后根据其氨基酸结构推算其相应的核苷酸序列,并据此合成寡核苷酸探针,最终从cDNA文库或基因组文库中筛选出目的基因。而未知其编码产物的发育基因的分离克隆则是非常困难的工作,以前广泛应用的主要方法有DNA标签法(DNAtagging)[1,2,3],作图克隆法(map-basedcloning)[4],差别筛选法(diferentialscreening)[5]和扣除杂交法(subtractivehybridization)[6,7,8]。这些方法虽然都取得了一定的成功,但由于各自的缺陷性,而限制了它们更加广泛的应用。近年来在PCR技术的基础上,人们已经建立了若干种分离克隆植物发育基因的新方法。1992年,LiangP和PardeeA[9]首次提出并运用了mRNA差别显示技术(mRNAdif-ferentialdisplayreversetranscription-PCR,DDRT-PCR)来进行基因的分离。实践表明,mRNA差别显示技术在分离、鉴定差别表达的新基因方面与以前的各种技术相比有其独特的优越性,但同...

过去20年,人们已经分离到了相当数量的与发育相关的基因。这主要借助于蛋白质产物的分离纯化,然后根据其氨基酸结构推算其相应的核苷酸序列,并据此合成寡核苷酸探针,最终从cDNA文库或基因组文库中筛选出目的基因。而未知其编码产物的发育基因的分离克隆则是非常困难的工作,以前广泛应用的主要方法有DNA标签法(DNAtagging)[1,2,3],作图克隆法(map-basedcloning)[4],差别筛选法(diferentialscreening)[5]和扣除杂交法(subtractivehybridization)[6,7,8]。这些方法虽然都取得了一定的成功,但由于各自的缺陷性,而限制了它们更加广泛的应用。近年来在PCR技术的基础上,人们已经建立了若干种分离克隆植物发育基因的新方法。1992年,LiangP和PardeeA[9]首次提出并运用了mRNA差别显示技术(mRNAdif-ferentialdisplayreversetranscription-PCR,DDRT-PCR)来进行基因的分离。实践表明,mRNA差别显示技术在分离、鉴定差别表达的新基因方面与以前的各种技术相比有其独特的优越性,但同时它也存在?

A powerful technology focusing on the investigation of differential expression of the genes controlling a series of life process, such as cell growth, cell differentiation, reproduction, senescence, programmed cell death ,oncogenesis and tolerance to environment stress was given. it will be helpful for our gene cloning research. In this paper,the general principle, procedure, advantages and shortcomings was summarized.

抑制性扣除杂交技术是一种基因克隆的新方法,它对研究细胞生殖、发育、分化、癌变、衰老及程序化死亡等生命过程有关基因的差异表达以及相关基因的分子克隆提供了有力的工具,本文简要介绍抑制性扣除杂交技术的主要原理,基本过程、优越性、主要缺陷及目前最新的研究进展

A natural flower homeotic mutant of Xanthoceras sorbifolia Bunge was studied by genome DNA subtraction between the mutant and wild plants. Through the subtraction, a wild plant specific genome DNA fragment has been found and this fragment will be used as a probe in following mutant gene screening study.

文冠果 (XanthoccrassorbifoliaBunge)同源异型变异株是自然产生的花器官发生变异的个体 .应用扣除杂交技术对该变异株和野生型植株进行了基因组差示杂交 ,获得样本特异DNA片段 ,此DNA片段可以作为进一步筛选突变基因的探针 ,为克隆文冠果花发育调控基因奠定基础

 
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