Based on the EST sequence of glutathione S-transferase gene from Tamarix androssowii (TaGST) published on GenBank, a TaGST cDNA was cloned with 3' and 5' RACE techniques. The full length of TaGST was 1 175 bp with ORF of 672 bp that encoded 224 amino acid residues(GenBank accission No. DQ683363).
A series of proteins of cytochrome b5 family were obtained using bioinformatics methods based on bovine liver microsomal cytochrome b5(CYB5-BOVIN), at the same time,the protein sequences were analyzed by alignment and evolution,which would provide instructional information for molecular design of cytochrome b5.
Isoschizomers were used to constructed multi-copy expression vectors and 2,3,4 multi-copy expression vectors pGAPZα-2hegf、pGAPZα-3hegf and pGAPZα-4hegf were successfully constructed,respectively. The results were a foundation for gene expression and bio-activity analysis.
Two dimensional electrophoresis (2-DE) was used to analyze the different proteins between the dwarf mutant (Dongze11) and its wild type (Dongnong42), which can be used to identified the gene related to the dwarf mutant.
Methods The plots method and drying weighting method were used to detect coefficients of reproductive allocation of 26 herbaceous plants in altitudes of 456 m,900 m,1 200 m,1 600 m and 2 000 m in Huoshan Mountain.