zymogram analysis was used to analyze the activity of MMP-2 and MMP-9. Results (1)In the process of stimulation by TGF-β_1,the type Ⅰ collagen mRNA level of 24 h,48 h and 72 h group was:1.33±0.07,2.46±0.09 and 2.39±0.08 respectivley;
Three types of xylanase (EC 220.127.116.11) were detected in the strain Aspergillus niger A-25 , one of which, designated as XynIII, also displayed a β-(1,3-1,4)-glucanase (EC 18.104.22.168) activity, as determined by zymogram analysis and cutting gel.
The changes in cerebral-cortex microvessel basement membrane structure and plasminogen activating system expression were observed with electron microscope,immunohistochemical method and zymogram analysis.
The cDNA fragment was amplified by RT PCR with specific primers. The purified RT PCR product was cloned in pGEM T Easy vector. The results of restriction endonuclease pattern analysis of the recombinant plasmid, DNA sequencing, and aligning of the putative amino acid sequence with hBD 1 and mBD 1 demonstrated that rat β defensin rBD 1 gene was cloned successfully.
68 plants regenerated in the dose range from 0.025 0 to 0.468 0 J/cm~2,and none at the dose of 0.960 0 J/cm~2.Results of chromosome counts,ploidy analysis by flow cytometry,isozyme analysis,RAPD and specific amplification(STS primers) of Ogura-CMS-associated mitochondrial gene orf138 revealed that 32 somatic hybrid plants were obtained.
By means of in situ electrophoresis chromosome,transformation, restriction map analysis and measurement of MIC,4 plasmids with different molecular weights and resistance to drugs obtained from the strain.
Restriction Enzyme Pattern Analysis of Mycobacteria DNA by Capillary Electrophoresis with Laser-Induced Fluorescence Detection
Compared with AGE, CE is more outstanding in resolution and detection time, and it can be applied as a more effective means to DNA restriction enzyme pattern analysis.
Based on the sampling data, a correct approach to the boundary effect on aggregation index was put forward to the spatial pattern analysis of the broad-leaved Korean pine forest in its different stages of development.
Point pattern analysis of different age-classes of Larix principis-rupprechtii in Luya Mountain Reserve, Shanxi Province, China
Spatial pattern analysis on individual trees in different age-classes of Larix principis-rupprechtii was made in this paper.