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酶谱分析     
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  zymogram analysis
     zymogram analysis was used to analyze the activity of MMP-2 and MMP-9. Results (1)In the process of stimulation by TGF-β_1,the type Ⅰ collagen mRNA level of 24 h,48 h and 72 h group was:1.33±0.07,2.46±0.09 and 2.39±0.08 respectivley;
     酶谱分析细胞上清液中MMP-2、MMP-9的活力。 结果(1)TGF-β1刺激HLF-02细胞,244、8、72 h组的Ⅰ型胶原mRNA表达水平分别为1.33±0.07、2.46±0.09、2.39±0.08;
短句来源
     Besides,comparison of MMP-2 and MMP-9 content in zymogram analysis in each group showed that changes of its quantity were in accordance with the laws of immune expression.
     另外,各组MMP-2与MMP-9的酶谱分析比较,其量的变化与免疫表达规律基本一致。
短句来源
     Three types of xylanase (EC 3.2.1.8) were detected in the strain Aspergillus niger A-25 , one of which, designated as XynIII, also displayed a β-(1,3-1,4)-glucanase (EC 3.2.1.73) activity, as determined by zymogram analysis and cutting gel.
     通过酶谱分析和切胶检测,发现黑曲霉A-25发酵液分别产生的三型木聚糖酶(命名为XynⅠ、XynⅡ和XynⅢ)和三型β-(1,3-1,4)-葡聚糖酶(命名为GluⅠ、GluⅡ、GluⅢ)。
短句来源
     The results of PAI-1 content in zymogram analysis among all groups were coincidence with above-mentioned results.
     各组PAI-1的酶谱分析比较,其量的变化与免疫表达结果基本一致。
短句来源
     The changes in cerebral-cortex microvessel basement membrane structure and plasminogen activating system expression were observed with electron microscope,immunohistochemical method and zymogram analysis.
     采用电镜、免疫组化和酶谱分析等法,观察各组脑微血管结构、纤溶酶原激活系的变化。
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  pattern analysis
     The cDNA fragment was amplified by RT PCR with specific primers. The purified RT PCR product was cloned in pGEM T Easy vector. The results of restriction endonuclease pattern analysis of the recombinant plasmid, DNA sequencing, and aligning of the putative amino acid sequence with hBD 1 and mBD 1 demonstrated that rat β defensin rBD 1 gene was cloned successfully.
     从大鼠肾脏组织提取RNA,采用RT-PCR技术扩增大鼠β-防御素rBD-1cDNA,并重组到pGEM-T Easy 载体,经限制性内切酶谱分析和DNA序列测定,证实所克隆的cDNA片段为大鼠β-防御素rBD-1 cDNA。
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  isozyme analysis
     68 plants regenerated in the dose range from 0.025 0 to 0.468 0 J/cm~2,and none at the dose of 0.960 0 J/cm~2.Results of chromosome counts,ploidy analysis by flow cytometry,isozyme analysis,RAPD and specific amplification(STS primers) of Ogura-CMS-associated mitochondrial gene orf138 revealed that 32 somatic hybrid plants were obtained.
     通过根尖染色体计数、流式细胞仪倍性鉴定、同工酶酶谱分析、RAPD及Ogu CMS线粒体基因orf138 STS(Sequence Tagged Site)标记引物扩增等方法对再生株进行了鉴定。 结果表明,其中32株为杂种植株。
短句来源
  map analysis
     By means of in situ electrophoresis chromosome,transformation, restriction map analysis and measurement of MIC,4 plasmids with different molecular weights and resistance to drugs obtained from the strain.
     通过对此菌进行染色体原位电泳、转化实验、限制性内切酶谱分析及MIC值的测定.从此菌中分离出四个具有不同分子量及耐药性的质粒。
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  zymogram analysis
Zymogram analysis for detection of xylanase activity after electrophoresis in polyacrylamide gels has shown thatA.
      
Zymogram analysis conducted with purified fractions from aerobic mycelium of YR-1 strain extracts indicated the existence of two AO enzymes (AO-1 and AO-2).
      
Zymogram analysis conducted with partially purified fractions from cell extracts from the aerobic mycelium of the YR-1 strain indicated the existence of only one oxygenase enzyme.
      
In previous work, zymogram analysis conducted with crude extracts from aerobic mycelium of YR-1 strain indicated the existence of two AO enzymes originally named AO-1 and AO-2.
      
Zymogram analysis with these extracts showed that this particular strain of M.
      
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  pattern analysis
Restriction Enzyme Pattern Analysis of Mycobacteria DNA by Capillary Electrophoresis with Laser-Induced Fluorescence Detection
      
Compared with AGE, CE is more outstanding in resolution and detection time, and it can be applied as a more effective means to DNA restriction enzyme pattern analysis.
      
Based on the sampling data, a correct approach to the boundary effect on aggregation index was put forward to the spatial pattern analysis of the broad-leaved Korean pine forest in its different stages of development.
      
Point pattern analysis of different age-classes of Larix principis-rupprechtii in Luya Mountain Reserve, Shanxi Province, China
      
Spatial pattern analysis on individual trees in different age-classes of Larix principis-rupprechtii was made in this paper.
      
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