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酶谱分析
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  “酶谱分析”译为未确定词的双语例句
    This included genetic improvement of strains by protoplast fusion and molecular biology and studies on genetic variability by zymogram technology and molecular genetics.
    其内容包括利用原生质体融合、基因工程等技术进行菌株遗传改良和利用酶谱分析、分子遗传学等技术研究其遗传变异。
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    The results of analyses of peroxidase isozymes showed that theactivity of enzymes in all varieties infected by p.
    过氧化物酶同工酶酶谱分析表明:感病后,不同抗病品种的酶带数均增加,抗病品种赤霞珠酶带数增加、着色深,而龙眼则酶带数少且着色淡。
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    It is thus believed that the Beijing Pediobius sp. is the same species as P. feveolatus from India.
    根据杂交试验和同工酶酶谱分析结果,可以推断北京地区的姬小蜂种名应为瓢虫姬小蜂(Pediobius foveolatus(Crawford)。
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    Meanwhile, the bands of peroxidase isozyme on PAGE increased as well.
    经过过氧化物同工酶酶谱分析,抗性再生植株酶谱带数有增加;
短句来源
    Specific PCR product was cloned into pGEM T Vector and the recombinant clone (pGEM \%St\%V) which contained PSt V cp gene was characterized with PCR screening 、restriction endonuclease mapping and nucleotide sequencing. The result showed that the similarities of nucleotide sequences and amino acid sequences between PSt V cp gene we cloned and other four published ones were above 96 3% and 97 5% respectively , suggesting that PSt V cp gene was very conservative.
    经限制酶谱分析后进行全序列测定,结果表明:该基因由1 084 个核苷酸组成,包含了PStVcp cDNA 完整的861 bp 编码序列( 编码287 个氨基酸)以及3'端223 bp 非编码序列,与文献报道的4 个PStVcp 基因相比同源性均在96 % 以上,且其编码的氨基酸同源性均在975 % 以上,说明PStVcp 基因具有较高的保守性。
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Pediobius sp. was obtained from the larvae and pupae of potato 28-spotted beetle, H. vigintioctomaculta. an important potato pest in Beijing. In a cross-mating test, this Pediobius species was found to have no reproductive isolation with P. feveolatus introduced from India. Electroreis studies using polyacrylamid gel showed that the esterase, lactate dehydrogenase, malic dehydrogenase and alcohol dehydrogenase of the two Pediobius populations were monomorphic, with only one different zone existed in the esterase....

Pediobius sp. was obtained from the larvae and pupae of potato 28-spotted beetle, H. vigintioctomaculta. an important potato pest in Beijing. In a cross-mating test, this Pediobius species was found to have no reproductive isolation with P. feveolatus introduced from India. Electroreis studies using polyacrylamid gel showed that the esterase, lactate dehydrogenase, malic dehydrogenase and alcohol dehydrogenase of the two Pediobius populations were monomorphic, with only one different zone existed in the esterase. It is thus believed that the Beijing Pediobius sp. is the same species as P. feveolatus from India.

杂交试验结果表明,北京地区寄生于马铃薯廿八星瓢虫幼虫和蛹的姬小蜂,与原产印度的瓢虫姬小蜂不存在生殖上的隔离。采用聚丙烯酰胺凝胶电泳方法对上述来源不同姬小蜂的同工酶作比较:两姬小蜂的乳酸脱氨酶(LDH)、苹果酸脱氢酶(MDH)和醇脱氢酶(ADH)同工酶谱均无差异,酯酶同工酶酶谱基本一致,表明两姬小蜂在上述同工酶的基因控制上,具有同一物种的遗传特征。根据杂交试验和同工酶酶谱分析结果,可以推断北京地区的姬小蜂种名应为瓢虫姬小蜂(Pediobius foveolatus(Crawford)。

The tuckahoe, Poria cocos (Fr. )Wolf, tends to degenerate after being cultured for 3-4 generations (asexual reproduction) on medium, a decrease in yield and quality. Long ago, many scientists had respected that screening fine strains and probe into a practical method could be resolved as mentiened above problems. Since 1985 screening to 9 strains introduced from Liaoning, Hubei, Hunan, Fujian and Anhui provinces has been made for 12 times. The results grow show that strain NO 5 (Hubei) and No 6 (Anhui) rapidly...

The tuckahoe, Poria cocos (Fr. )Wolf, tends to degenerate after being cultured for 3-4 generations (asexual reproduction) on medium, a decrease in yield and quality. Long ago, many scientists had respected that screening fine strains and probe into a practical method could be resolved as mentiened above problems. Since 1985 screening to 9 strains introduced from Liaoning, Hubei, Hunan, Fujian and Anhui provinces has been made for 12 times. The results grow show that strain NO 5 (Hubei) and No 6 (Anhui) rapidly and the qualities are fine when cultured on PDA with 20g/1 boil liquid of pine root at 28℃, In order to overcome the degeneration caused by asexual reproduction, the old curtures incubated for 20 days and subcultured for 3-4 generations were re-incubated at above 28℃ and RH 85% for 7-10 days, the fruiting bodies formed on plate or in test tube, and then, the basidiospores were collected for sexual reproduction. Application of this method resolved (?)cally the degeneration caused by asexual reproduction. Besides the characteristics of strains, esterase isoenzymes were tested. The results indicate that strain No 1 has 3 enzyme bands (including strong, mid, weak) on enzyme pattern, and the enzyme activity is stronger than others, and proves to be the fine strain for the strain screening.

对辽宁、河南、湖北、湖南、福建和安徽各地引进的9个茯苓[Poria cocos(Fr)Wolf]菌株进行了12次筛选,结果证明,用PDA加20克/升松根煮液的培养基、在28℃下培养的编号5—(湖北)和编号6—(安徽)菌株,生长快、质地好。将转管3—4次、培养达20天的菌种,置于28℃以上、湿度85%以上,继续培养7—10天,形成子实体,进行有性繁殖,基本上解决了茯苓无性繁殖菌种易老化的问题。从各菌株的酯酶同工酶酶谱分析可以看出:有性繁殖中编号1菌株,具强、中、弱三条酶带、酶的活性强,在各菌株中最为优良。

The easterase isoenzymes and soluble proteins of normal hermaphroditic isolates in Pyricu-laria oryzae Cav. were analyzed by CC+PAGE (gradient concentration polyacrylamide gel elec-trophoresis). The results showed that easterase zymograms were relatively stable and isoenzyme nands ranged gradually from 4% to 26% gel in same cultural condition. The zymograms of Mating type A were quite different from Mating type a, and SC (similar cofficient) was 0.762-0.857. The numbers of soluble protein band were between 22-25,...

The easterase isoenzymes and soluble proteins of normal hermaphroditic isolates in Pyricu-laria oryzae Cav. were analyzed by CC+PAGE (gradient concentration polyacrylamide gel elec-trophoresis). The results showed that easterase zymograms were relatively stable and isoenzyme nands ranged gradually from 4% to 26% gel in same cultural condition. The zymograms of Mating type A were quite different from Mating type a, and SC (similar cofficient) was 0.762-0.857. The numbers of soluble protein band were between 22-25, ranging from 5% to 27% in gel concentration. The diversity of those gel scanning patterens was obvious. It is suggested that easterase zymogram or soluble protein profile analysis is aeffective method to discriminate mating type of herma-phroditic isolate of P. oryzace Cav. Apart from above, the significance of isoenzyme analysis technique on evolution and classification of P. oryzae Cav. is discussed in this paper.

应用聚丙烯酰胺梯度浓度凝胶电泳法对稻瘟病菌两性菌株的菌体可溶性蛋白质和酯酶同工酶进行了分析比较。结果表明:相同培养条件下的两性菌株的菌体酯酶酶谱相对稳定,同工酶谱带相应分布在凝胶浓度14~26%范围内;交配型A的酯酶谱带明显区别于交配型a,两者相似系数为0.762~0.857;电泳结果,两性菌株的菌体可溶性蛋白质谱带有22~25条,分布在凝胶浓度5~26%范围内;研究结果认为酯酶酶谱或可溶性蛋白质图谱可以作为初步、快速鉴别两性菌株交配型的有效手段。此外,本文还初步探讨了酶谱分析技术在稻瘟病菌分类和演化研究上的意义。

 
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