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酶谱分析
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  “酶谱分析”译为未确定词的双语例句
    Cloning and Mapping of Hind Ⅲ Fragments of Egg Drop Syndrome Virus Genome
    减蛋综合征病毒基因组Hind Ⅲ酶切片段的克隆及酶谱分析
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    Here we report the identification of a novel β–defensin from camel tisses,camel β–defensin-1(caBD-1). Based on the conserved sequence of other cud chewer,the primer were designed. Total RNA was extracted from the tongue epithelia of camel and the cDNA encoding caml β–defensin (caBD-1) was amplified by the reverse transcription PCR(RT-PCR).
    我们利用其他反刍动物(牛和羊)cDNA的保守序列设计引物,从骆驼舌黏膜上皮组织中提取总RNA,采用RT-PCR技术扩增出caBD-1(camelβ-defensin-1)的cDNA,并重组到pBlueselect T载体,经限制性内切酶谱分析和DNA序列测定,证实所克隆的caBD-1的cDNA为β-防御素。
短句来源
    Cloning and Mapping of the Restricted Genomical Fragments of Fowlpox virus 282E4 Strain
    鸡痘病毒基因组酶切片段的克隆及酶谱分析
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    Cloning and Mapping of the Aeromonas hydrophila HEC Toxin Gene Determinant
    嗜水气单胞菌HEC毒素基因的克隆及酶谱分析
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    Through analyzing two kinds of isoenzyme, MDH and LDH, different breeds has specific brands.
    同工酶分析已被广泛的应用于物种分析,因此本实验对所建细胞系进行了乳酸脱氢酶(LDH)与苹果酸脱氢酶(MDH)的酶谱分析,表明不同物种各呈现其独特的条带,具有种属特异性。
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To localize the gene encoding the thymidine kinase(tk) gene from a virulent Chinese field isolate of ILTV, a library of the Chinese ILTV strain genome was constructed in the pBluescript SK+ Vector. A pair of primers from the sequence data of tk gene in the virulent US isolate of ILTV were used in the PCR to amplify a 713 base pair(bp) tk fragment of DNA from Chinese strain of ILTV. The 713 bp DNA fragment was used in dot-blot and Southern blot to screen the recombinant plasmid of the tk from the Chinese ILTV...

To localize the gene encoding the thymidine kinase(tk) gene from a virulent Chinese field isolate of ILTV, a library of the Chinese ILTV strain genome was constructed in the pBluescript SK+ Vector. A pair of primers from the sequence data of tk gene in the virulent US isolate of ILTV were used in the PCR to amplify a 713 base pair(bp) tk fragment of DNA from Chinese strain of ILTV. The 713 bp DNA fragment was used in dot-blot and Southern blot to screen the recombinant plasmid of the tk from the Chinese ILTV strain and to localize the ILTV tk gene on a 2. 4 kb Hind III fragment of the Chinese ILTV strain. It was domonstrated that the restrict map of tk gene from the virulent US isolate of ILTV was in accord with the restrict map of the tk gene from the virulent Chinese isolate of ILTV.

以质粒pBluescript SK~+为载体克隆传染性喉气管炎病毒(ILTV)中国王岗株的DNA,构建了THind Ⅲ DNA文库。参考ILTV美国632株胸苷激酶(tk)基因的DNA序列,设计并合成了一对20bp长的引物;以ILTV中国王岗株DNA为模板,用PCR方法特异性地扩增出ILTV-713bp的tk DNA片段。用光敏生物素标记该713bp片段作为探针,通过斑点杂交从ILTV中国王岗株Hind Ⅲ DNA文库中筛选出4个含2.4kb外源片段的tk基因阳性重组质粒。经酶切分析、Southern杂交、PCR检测和部分限制酶酶谱分析表明:ILTV中国王岗株DNA 2.4 kb Hind Ⅲ 片段无论是片段大小还是酶切图谱均与美国632株完全相同,而且Southern杂交和PCR检测均为tk阳性,证明其中包含有完整的tk基因。本研究的目标是用克隆的tk基因进行体外缺失和重组,以构建毒力进一步降低的tk缺失减毒株,这些工作正在进行中。

Abstract This paper presented that twenty three viral specific cloned fragments were mapped after EcoRⅠand EcoRⅠ/Hind Ⅱ fragment libriary was established.Typical pox vinous purified by sucrose densitygradient centrifugation were observed under electron microscope after PTA-negative staining samples prepaired from two opalescent bands in the gradient column,but no sisnificant difference was detected between the preparation from the above banded samples which may be corelated with the two forms of fowlpox virus,the...

Abstract This paper presented that twenty three viral specific cloned fragments were mapped after EcoRⅠand EcoRⅠ/Hind Ⅱ fragment libriary was established.Typical pox vinous purified by sucrose densitygradient centrifugation were observed under electron microscope after PTA-negative staining samples prepaired from two opalescent bands in the gradient column,but no sisnificant difference was detected between the preparation from the above banded samples which may be corelated with the two forms of fowlpox virus,the intracellular and extracellular form.The genomical DNA Shared typical feature of agsrose electrophretic behaviour of the high-molecule-weight.DNA were restricted with EcoRⅠor EcoRⅠ/Hind Ⅲ followed by ligation with the linerized pUC19 plasmid then transforming the E.colt.JM109 strain competent cells.Restficted fragment clone libriary was established after screening out the recombinants by colony color selection and AGE size fraction. Viral specific clones were further identified by in siu hybridization and dot blotting with Digeoxnin-labelled CEF DNA or FPV DNA probe.TOtally twenty three recombinant plasmids were mapped with the restriction endonucleases within the polylinker and no cleavable sites in pUC19 according to the double-enzyme-digotion method.Available insertion sit6s for further incorporation of the reporter gene cassette was located in each of the above cloned genomical fragments.This will be greatly facilitated the shotgun screening of the nonessential-region(NER)for virus replication as weil as the molecular virological study of PPV and its engineering as an expression vector.

以鸡胚成纤维细胞培养的中国鸡痘病毒鹌鹑化弱毒株,经蔗糖密度梯度离心法纯化,电镜观察示所得病毒为典型鸡瘟病毒颗粒。以蛋白酶K法抽提病毒基因组,电泳结果为典型的大分子量DNA特征,经EcoRI或EcoRI/HindⅢ酶切,插入pUC19载体相应位点,经转化、筛选、鉴定后得相应酶切片段的基因组文库。对其中23个克隆片段用双酶法进行酶谱分析,绘制出了相应克隆片段的酶切图谱,每个克隆片段中均有1~6个可以利用的插入位点。基因组文库的构建及部分克隆片段的酶谱分析为进一步筛选病毒复制非必需片段以构建重组疫苗表达载体乃至鸡痘病毒分子生物学的研究打下了坚实的物质基础。

The genome library of a Chinese strain of ILTV was constructed in the pUC19 vector. Following the sequence data of gX gene in SA2 strain of ILTV a pair of primers was used in the PCR to amplify a 841 bp gX gene fragment of DNA of Chinese strain of ILTV. The 841 bp DNA fragment was labelled by DIG as a probe. The probe was used in dot blot and Southern hybridization to screen the recombinant of the gX gene from the Chinese strain of ILTV and to localize the ILTV gX gene in a 5.2 kb KpnⅠ DNA fragment of Chinese...

The genome library of a Chinese strain of ILTV was constructed in the pUC19 vector. Following the sequence data of gX gene in SA2 strain of ILTV a pair of primers was used in the PCR to amplify a 841 bp gX gene fragment of DNA of Chinese strain of ILTV. The 841 bp DNA fragment was labelled by DIG as a probe. The probe was used in dot blot and Southern hybridization to screen the recombinant of the gX gene from the Chinese strain of ILTV and to localize the ILTV gX gene in a 5.2 kb KpnⅠ DNA fragment of Chinese strain of ILTV. It was demonstrated that the restriction map of gX gene of the Chinese strain of ILTV was identical with that of SA2 strain of ILTV.

以pUC19质粒为载体克隆鸡传染性喉气管炎病毒(ILTV)中国王岗株的DNA,构建了鸡传染性喉气管炎病毒DNA的KpnⅠDNA文库。参考ILTV-SA2株gX基因的核酸序列,设计并合成了分别为12bp和13bp的1对引物。以ILTV中国王岗株DNA为模板,用PCR方法特异性地扩增出0.84kb的ILTV-gX基因片段。以地高辛标记该0.84kb的片段为探针,经Southern杂交从ILTV中国王岗株KpnⅠDNA文库中筛选出3个含5.2kb外源ILTVDNA片段的gX基因阳性重组子。经酶切分析、Southern杂交、PCR检测和该片段部分酶谱分析表明,ILTV中国王岗株DNA5.2kb的KpnⅠ片段无论是片段大小还是酶切图谱均与ILTV-SA2株完全相同,而且Southern杂交和PCR检测均为gX阳性,证明其中含有完整的gX基因

 
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