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工作稀释
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     1: 10, 000-20000 in PAP and ABC methods,The stainings were with a very low background.
     其工作稀释浓度IF法为1:4000~8000,PAP及ABC法为1:10,000~20,000,染色背景低。
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     ml-1 and 1.0×105,respectively.
     ml-1,抗血清的工作稀释倍数为1.0×105。
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  相似匹配句对
     on working
     在工作
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     Part-time Job
     业余工作
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     Diluted Chinese Style
     稀释中式
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     THE DILUTION REFRIGERATOR
     稀释致冷机
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     Experimental study on working parameters of XHJ-Ⅱ mixer
     XHJ-Ⅱ型稀释混合机合理工作参数的试验研究
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This article has first described the early diagnosis of Schistosomiasis japonica in rabbits using self-made Alkaline phosphatase (sm-AKP)-ELisA indirect method. ElisA-plates coated with Schistosoma japonicum egg crude antigen(Ag-0)maintained at 4μg/ml, 0.2ml/hole, and incubated over night at 4℃, the serum of the normal rabbits and Schistosomiasis rabbits diluted to 1:200and 1:400 respectively, the working dilution of sm-AKP-goat antirabbit-IgG 1:200 were used as optimum condition of the rabbits to be tested....

This article has first described the early diagnosis of Schistosomiasis japonica in rabbits using self-made Alkaline phosphatase (sm-AKP)-ELisA indirect method. ElisA-plates coated with Schistosoma japonicum egg crude antigen(Ag-0)maintained at 4μg/ml, 0.2ml/hole, and incubated over night at 4℃, the serum of the normal rabbits and Schistosomiasis rabbits diluted to 1:200and 1:400 respectively, the working dilution of sm-AKP-goat antirabbit-IgG 1:200 were used as optimum condition of the rabbits to be tested. Five were inoculated with 50 cercariae of Schistosoma japonicum and the other five infected with 1200. The rabbits' serum was collected at intervals of 3 days after infection. The serum of the ten experimental rabbits was detected by ELisA. 12 days after infection. the serum of all the rabbits showed positive. Using the above optimum condition we detected the serum of the five Clonorchiasis rabbits by ELisA. All tunned negative. We think that sm-AKP-ELisA indirect method is of diagnostic value for early Schistoso-miasis japonica in rabbits and has a definite specific activity.

本文报道自产碱性磷酸酶(自产AKP)ELisA间接法对日本血吸虫病兔的早期诊断。采用日本血吸虫卵粗抗原4μg/ml,每孔滴入0.2ml,4℃包板过夜,并以正常兔血清1:200,日本血吸虫病兔血清1:400,自产AKP——羊抗兔IgG工作稀释度1:200,为适宜条件。受试家兔中5只感染日本血吸虫尾蚴50条,另5只感染1200条。感染后,每隔三天,进行ELisA检测。结果:受试家兔血清于感染后第12天全部出现阳性;同时,采用上述适宜条件,进行ELisA检测5只肝吸虫病兔血清,结果全为阴性。显示自产AKP—ELisA间接法检测日本血吸虫病兔具有早期诊断的价值,并有一定的特异性。

Properties of the homemade serotonin (5-HT) antibody were determined by immunogoldsilver staining method on the paper models. There was a good linear relation between dilution of the antibody and least amount of 5-HT shown by the antibody and the antibody diluted 1: 10000 could show 82.50ng 5-HT. The antibody could be absorbed by excess of the HSA-5-HT but could not be absorbed by excess of histamine and triptophane.These results suggested that the homemade 5-HT antibody possessed high sensitivity in immunohistochemical...

Properties of the homemade serotonin (5-HT) antibody were determined by immunogoldsilver staining method on the paper models. There was a good linear relation between dilution of the antibody and least amount of 5-HT shown by the antibody and the antibody diluted 1: 10000 could show 82.50ng 5-HT. The antibody could be absorbed by excess of the HSA-5-HT but could not be absorbed by excess of histamine and triptophane.These results suggested that the homemade 5-HT antibody possessed high sensitivity in immunohistochemical reaction.

本文用纸模的免疫金银法鉴定了自制5-HT抗体的性质。抗体的工作稀释度与其能显示的最小抗原量呈线性关系,1:10000稀释度的抗体能显示22.50ng的5-HT。该抗体能被过量的HSA-5-HT完全吸收,但不被过量的组织胺和色氨酸所吸收。以上结果表明表明,该抗体在免疫组化反应中有较高的敏感性和特异性。

Refer to the Morz's method to preparate SP antigen, The antibody was raised in six rabbits against synthetic SP, The RIA titre of N.Io SP antibody was 1: 150, 000-180, 000, The average affinity constant of the antibody was kd=3.5×10~(10) L/M.as determined by Scatchard plot analysis. Immunocytochemical techniques were used to determine the sensitivity and specificely of N. IO SP antibody, The working dilution was Ⅰ: 4000-8000 in IF method ; 1: 10, 000-20000 in PAP and ABC methods,The stainings were with a very...

Refer to the Morz's method to preparate SP antigen, The antibody was raised in six rabbits against synthetic SP, The RIA titre of N.Io SP antibody was 1: 150, 000-180, 000, The average affinity constant of the antibody was kd=3.5×10~(10) L/M.as determined by Scatchard plot analysis. Immunocytochemical techniques were used to determine the sensitivity and specificely of N. IO SP antibody, The working dilution was Ⅰ: 4000-8000 in IF method ; 1: 10, 000-20000 in PAP and ABC methods,The stainings were with a very low background.The distribution and morphology of SP-immunoreactive nerve fibers in the spinal cord, gastrointestinal tract, Pancrease and adrenal gland etc.of rat and guinea-pig stained with N.IO SP antibody were similar to other laboratory's reports. It is showed that N.IO SP antibody was suitable for immunocytochemistry and radioimmunoassay studies.

参照Mroz的方法制备P——物质抗原,用六只家兔免疫制备P物质抗体。经RIA测定N.10SP抗体漓度为1:150,000——180,000.用Scatchard作图求得抗体亲和常数Kd=3.5×10~(10)L/M。以免疫细胞化学染色检验抗体之特异性灵敏性。其工作稀释浓度IF法为1:4000~8000,PAP及ABC法为1:10,000~20,000,染色背景低。P物质免疫反应神经纤维在大鼠及豚鼠的脊髓、胃肠道、胰、肾上腺等部位之形态与分布和文献报导相似。实验表明,自制N.10SP抗体可适用于放射免疫测定和免疫细胞化学染色。

 
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