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精子处理
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  sperm treatment
     The successful rate was influenced by sperm treatment, insemination technic,insemination time,and the fertile function of the receiver etc.
     讨论认为本方法对不育症有实际治疗作用,成功率受到精子处理、授精技术、授精时机、受精妇女生育功能等因素影响.
短句来源
  “精子处理”译为未确定词的双语例句
     Experiment 1, four treatment groups were involved: (1) untreated sperm as control;
     实验1包括4个精子处理组:(1)0处理组(对照组);
短句来源
     These are the changes of percentage of progressively moving spermatozoa(PMS),the human sperm hypoosmotic swelling test(HOS), the percentage of spermatonoa with intact double stranded DNA(ESC) (were evaluated) before and after the treatment.
     方法 采用精子前向运动试验 (PMS)、精子尾部低渗肿胀试验 (HOS)和DNA荧光染色有效精子计数试验 (ESC) ,观察精子处理前后PMS ,HOS和ESC的变化 .
短句来源
     Relationship between acid phosphatase and non-specific esterase in rat epididymal epithelium and digested excessive spermatozoa
     大鼠附睾上皮内ACP及NSE与过剩精子处理的关系初探
短句来源
     Methods Sperm parameters before and after preparation,fertilization rate of mature oocytes and embryo morphology were observed to study the influence of sperm morphology on the fertilization rate and the development of embryo.
     方法 观察 89个IVF ET周期精子处理前后各项参数及成熟卵的受精率、卵裂胚胎的细胞数及形态 ,分析不同受精密度下精子形态对受精率及胚胎发育的影响。
短句来源
  相似匹配句对
     Changes of sperm quality in male mice treated with manganous chloride
     氯化锰处理小鼠精子质量的变化
短句来源
     Studies on in vitro Capacitation of Goat Spermatozoa by Heparin Treatment
     肝素处理山羊精子体外获能的研究
短句来源
     TREATMENT OF ION EXCHANGE RESINS
     离子交换树脂的处理
短句来源
     R. japoncnsis was treated as a variety under R.
     japonensis处理为R.
短句来源
     A MICROCOMPUTER-BASED IMAGE ANALYSIS SYSTEM FOR HUMAN SPERM QUALITY ANALYSIS
     精子自动分析系统
短句来源
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  sperm treatment
Sperm treatment with dimethylsulphate yields haploid embryos containing residual sperm chromatin fragments, in contrast to treatment with ultraviolet rays.
      
Changes in both supernates and pellets must have occurred after 1MA and sperm treatment.
      
Sperm treatment consisted of incubation of 15 x 106 sperm with 1.05 mg uterine fluid protein for indicated intervals.
      
With the advent of ICSI, the most common situation for sperm treatment is now enhancement for IUI.
      


The influences of different treatment methods such as living spermatozoon, spermatozoa smear without fixation and different fixing methods on determination effect of immunofluorescence (indirect method) and the conservation of spermatozoon antigenicity are compared. Immunofiuorescent method is applied to select hybridoma cell system which secretes antispermatozoon monoclonal antibody, all kinds of methods of spermatozoon treatment can be used.Although atrong fluorescence can be obtained by us- ing living spermatoza,...

The influences of different treatment methods such as living spermatozoon, spermatozoa smear without fixation and different fixing methods on determination effect of immunofluorescence (indirect method) and the conservation of spermatozoon antigenicity are compared. Immunofiuorescent method is applied to select hybridoma cell system which secretes antispermatozoon monoclonal antibody, all kinds of methods of spermatozoon treatment can be used.Although atrong fluorescence can be obtained by us- ing living spermatoza, but it is often to fail because of the spermia loss in procedure.To de- termine monoclonal antibody combined with the sperm tail, it is better that the smear is not fixed. However, to determine monoclonal antibody combined with the sperm head, the favourable way is to fix it with acefone or 4℃ ethyl alcohol.If the smear is not fixed, antigenicity of spermatozoa is easy to change, and the antigenicity can be kept for more six monthes after fialng.

本文比较了用活精子、精子涂片不加固定及不同固定方法对间接免疫荧光检测效果的影响和精子抗原性保存问题。应用免疫荧光法筛选分泌抗精子单克隆抗体杂交瘤细胞系,各种精子处理方法均可采用。但用活精子常因操作中精子丢失而失败;检测与精子尾部结合的单克隆抗体,最好不加固定;检测与精子头部结合的单克隆抗体,用丙酮或4℃乙醇固定为佳;涂片不加固定抗原性易改变,而固定后其抗原性可保持6个月以上。

In vitro fertilization and embryotransfer (IVF-ET) is an useful way to study mechanism of reproduction, for instance, the regulation of oocytematuration, the role of maturation and development of fertilized eggs , embryo implantation as well as a genetic disease model for human. The technology is also helpful for conservation endangered species in nonhuman primates and enhance the quality for laboratory primates. This paper was summarized the progress of IVF- ET in nonhuman primates including oocytes got from...

In vitro fertilization and embryotransfer (IVF-ET) is an useful way to study mechanism of reproduction, for instance, the regulation of oocytematuration, the role of maturation and development of fertilized eggs , embryo implantation as well as a genetic disease model for human. The technology is also helpful for conservation endangered species in nonhuman primates and enhance the quality for laboratory primates. This paper was summarized the progress of IVF- ET in nonhuman primates including oocytes got from hyperstimulation and unstimulated animals, semen collection, semen cryopreservation, fertilization in vitro, embryo transfer and embryo cryopreservation.

非人灵长类的体外受精和胚胎移植是了解人类生殖机制,如卵的成熟调控,受精卵的成熟与分化,胚胎着床,控制某些遗传疾病以及保护珍稀灵长类和提高实验灵长类质量的有效途径。本文从非人灵长类卵的获取(包括超数排卵,非激素刺激动物取卵),精子处理(精液采集,冻存和精子获能),体外受精和胚胎移植、胚胎的冻存等方面介绍了有关研究概况和发展动态。

Hoechst 33342 and laser are necessarily employed to treat sperm when flow cytometry is used to separate sperm into X-and Y-chromosome bearing populations. Two experiments were conducted to determine the effects of dye (Hoechst 33342) and laser on X and Y sperm and their subsequent in vitro fertilization (IVF) and culture (IVC) of bovine oocytes to blastocyst (BL) and hatched BL stages. Experiment 1, four treatment groups were involved: (1) untreated sperm as control;(2) sperm stained with Hoechst 33342 at a...

Hoechst 33342 and laser are necessarily employed to treat sperm when flow cytometry is used to separate sperm into X-and Y-chromosome bearing populations. Two experiments were conducted to determine the effects of dye (Hoechst 33342) and laser on X and Y sperm and their subsequent in vitro fertilization (IVF) and culture (IVC) of bovine oocytes to blastocyst (BL) and hatched BL stages. Experiment 1, four treatment groups were involved: (1) untreated sperm as control;(2) sperm stained with Hoechst 33342 at a level of 9 mg/L only; (3) unstained sperm treated with laser at a level of 150 mW only; (4) sperm treated with both Hoechst 33342 and laser. IVF with sperm of the four treatments and IVC were carried out according to the method described by Lu et al (1988). Experiment 2, different concentrations of Hoechst 33342 were used to investigate the relationship between dye concentration and the results of IVF and IVC.It was found in this study that Hoechst 33342 significantly reduced rates of cleavage,BL,grade one BL,hatched BL and BL developmental speed. These effects, except the effect on cleavage rate, were greater when the concentration of Hoechst 33342 was higher. No effect of laser was found on the results of IVF.

用流动细胞计数法分离牛的X,Y精子,需要对精子进行活体荧光染色及激光照射处理。本研究进行2个实验,以探索荧光染料(Hoechst33342)和激光对牛精子以及牛卵母细胞的体外受精和胚胎的体外培养的影响。实验1包括4个精子处理组:(1)0处理组(对照组);(2)染色组,用9mg/L的Hoechst33342对精子进行染色;(3)激光照射组,用强度为150mW的激光对精子进行照射;(4)染色及激光照射组,综合(2)和(3)的处理。实验2用不同浓度的Hoechst33342处理精子,以研究染料浓度与体外受精效果的关系。实验结果表明,Hoechst33342作用于精子之后,能显著降低牛卵母细胞体外受精后的分裂率、囊胚率、一级囊胚率、囊胚孵化率及胚胎发育速度,而且降低的幅度随着染料浓度的升高而增加(卵裂率除外)。精子经激光照射后对体外受精的效果没有显著影响。

 
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